6b5o - Revision history

OCA at 21:36, 28 October 2020

OCA — Wed, 28 Oct 2020 21:36:51 GMT

←Older revision		Revision as of 21:36, 28 October 2020
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	==Structure of PfCSP peptide 29 with human protective antibody CIS43==		==Structure of PfCSP peptide 29 with human protective antibody CIS43==
-	<StructureSection load='6b5o' size='340' side='right' caption='[[6b5o]], [[Resolution\|resolution]] 2.19Å' scene=''>	+	<StructureSection load='6b5o' size='340' side='right'caption='[[6b5o]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b5o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B5O OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6B5O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b5o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B5O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6B5O FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6b5l\|6b5l]], [[6b5m\|6b5m]], [[6b5n\|6b5n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6b5l\|6b5l]], [[6b5m\|6b5m]], [[6b5n\|6b5n]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6b5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b5o OCA], [http://pdbe.org/6b5o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b5o RCSB], [http://www.ebi.ac.uk/pdbsum/6b5o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b5o ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6b5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b5o OCA], [http://pdbe.org/6b5o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b5o RCSB], [http://www.ebi.ac.uk/pdbsum/6b5o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b5o ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Pancera, M]]		[[Category: Pancera, M]]
	[[Category: Weidle, C]]		[[Category: Weidle, C]]

OCA at 06:55, 4 April 2018

OCA — Wed, 04 Apr 2018 06:55:23 GMT

←Older revision		Revision as of 06:55, 4 April 2018
Line 3:		Line 3:
	<StructureSection load='6b5o' size='340' side='right' caption='[[6b5o]], [[Resolution\|resolution]] 2.19Å' scene=''>		<StructureSection load='6b5o' size='340' side='right' caption='[[6b5o]], [[Resolution\|resolution]] 2.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b5o]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B5O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B5O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b5o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B5O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B5O FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6b5l\|6b5l]], [[6b5m\|6b5m]], [[6b5n\|6b5n]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6b5l\|6b5l]], [[6b5m\|6b5m]], [[6b5n\|6b5n]]</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b5o OCA], [http://pdbe.org/6b5o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b5o RCSB], [http://www.ebi.ac.uk/pdbsum/6b5o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b5o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b5o OCA], [http://pdbe.org/6b5o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b5o RCSB], [http://www.ebi.ac.uk/pdbsum/6b5o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b5o ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Development of a highly effective vaccine or antibodies for the prevention and ultimately elimination of malaria is urgently needed. Here we report the isolation of a number of human monoclonal antibodies directed against the Plasmodium falciparum (Pf) circumsporozoite protein (PfCSP) from several subjects immunized with an attenuated Pf whole-sporozoite (SPZ) vaccine (Sanaria PfSPZ Vaccine). Passive transfer of one of these antibodies, monoclonal antibody CIS43, conferred high-level, sterile protection in two different mouse models of malaria infection. The affinity and stoichiometry of CIS43 binding to PfCSP indicate that there are two sequential multivalent binding events encompassing the repeat domain. The first binding event is to a unique 'junctional' epitope positioned between the N terminus and the central repeat domain of PfCSP. Moreover, CIS43 prevented proteolytic cleavage of PfCSP on PfSPZ. Analysis of crystal structures of the CIS43 antigen-binding fragment in complex with the junctional epitope determined the molecular interactions of binding, revealed the epitope's conformational flexibility and defined Asn-Pro-Asn (NPN) as the structural repeat motif. The demonstration that CIS43 is highly effective for passive prevention of malaria has potential application for use in travelers, military personnel and elimination campaigns and identifies a new and conserved site of vulnerability on PfCSP for next-generation rational vaccine design.
		+
		+	A human monoclonal antibody prevents malaria infection by targeting a new site of vulnerability on the parasite.,Kisalu NK, Idris AH, Weidle C, Flores-Garcia Y, Flynn BJ, Sack BK, Murphy S, Scho N A, Freire E, Francica JR, Miller AB, Gregory J, March S, Liao HX, Haynes BF, Wiehe K, Trama AM, Saunders KO, Gladden MA, Monroe A, Bonsignori M, Kanekiyo M, Wheatley AK, McDermott AB, Farney SK, Chuang GY, Zhang B, Kc N, Chakravarty S, Kwong PD, Sinnis P, Bhatia SN, Kappe SHI, Sim BKL, Hoffman SL, Zavala F, Pancera M, Seder RA Nat Med. 2018 Mar 19. pii: nm.4512. doi: 10.1038/nm.4512. PMID:29554083<ref>PMID:29554083</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6b5o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Pancera, M]]		[[Category: Pancera, M]]
	[[Category: Weidle, C]]		[[Category: Weidle, C]]

OCA at 07:38, 21 March 2018

OCA — Wed, 21 Mar 2018 07:38:43 GMT

←Older revision		Revision as of 07:38, 21 March 2018
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-	'''Unreleased structure'''

-	~~The entry 6b5o is ON HOLD~~	+	==Structure of PfCSP peptide 29 with human protective antibody CIS43==
-		+	<StructureSection load='6b5o' size='340' side='right' caption='[[6b5o]], [[Resolution\|resolution]] 2.19Å' scene=''>
-	~~Authors: Pancera, M., Weidle, C.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6b5o]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B5O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B5O FirstGlance]. <br>
-	~~Description:~~ Structure of ~~pfCSP~~ peptide 29 with human protective antibody CIS43	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6b5l\|6b5l]], [[6b5m\|6b5m]], [[6b5n\|6b5n]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b5o OCA], [http://pdbe.org/6b5o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b5o RCSB], [http://www.ebi.ac.uk/pdbsum/6b5o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b5o ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Pancera, M]]		[[Category: Pancera, M]]
	[[Category: Weidle, C]]		[[Category: Weidle, C]]
		+	[[Category: Antibody]]
		+	[[Category: Immune system]]
		+	[[Category: Malaria]]
		+	[[Category: Pfcsp]]
		+	[[Category: Vaccine]]

OCA: Protected "6b5o" [edit=sysop:move=sysop]

OCA — Wed, 11 Oct 2017 05:30:07 GMT

Protected "6b5o" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:30, 11 October 2017

OCA: New page: '''Unreleased structure''' The entry 6b5o is ON HOLD Authors: Pancera, M., Weidle, C. Description: Structure of pfCSP peptide 29 with human protective antibody CIS43 [[Category: Unrele...

OCA — Wed, 11 Oct 2017 05:30:05 GMT

New page: '''Unreleased structure''' The entry 6b5o is ON HOLD Authors: Pancera, M., Weidle, C. Description: Structure of pfCSP peptide 29 with human protective antibody CIS43 [[Category: Unrele...

New page

'''Unreleased structure'''

The entry 6b5o is ON HOLD

Authors: Pancera, M., Weidle, C.

Description: Structure of pfCSP peptide 29 with human protective antibody CIS43
[[Category: Unreleased Structures]]
[[Category: Pancera, M]]
[[Category: Weidle, C]]