6b6i - Revision history

OCA at 14:33, 4 October 2023

OCA — Wed, 04 Oct 2023 14:33:44 GMT

←Older revision		Revision as of 14:33, 4 October 2023
Line 1:		Line 1:

	==2.4A resolution structure of human Norovirus GII.4 protease==		==2.4A resolution structure of human Norovirus GII.4 protease==
-	<StructureSection load='6b6i' size='340' side='right' caption='[[6b6i]], [[Resolution\|resolution]] 2.44Å' scene=''>	+	<StructureSection load='6b6i' size='340' side='right'caption='[[6b6i]], [[Resolution\|resolution]] 2.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b6i]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Norovirus_gii~~.4 Norovirus ~~gii~~.4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6B6I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b6i]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Norovirus_GII.4 Norovirus GII.4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B6I FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b6i OCA], [~~http~~://pdbe.org/6b6i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6b6i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6b6i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6b6i ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.44Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b6i OCA], [https://pdbe.org/6b6i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b6i RCSB], [https://www.ebi.ac.uk/pdbsum/6b6i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b6i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~A0A076EGG6_9CALI A0A076EGG6_9CALI]~~] 3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave polyadenylate-binding protein thereby inhibiting cellular translation.[PROSITE-ProRule:PRU00870]	+	[https://www.uniprot.org/uniprot/A0A0K2E2J2_9CALI A0A0K2E2J2_9CALI] 3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave polyadenylate-binding protein thereby inhibiting cellular translation.[PROSITE-ProRule:PRU00870] NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity.[ARBA:ARBA00025124] Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation.[ARBA:ARBA00025359]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 6b6i" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6b6i" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Virus protease 3D structures\|Virus protease 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Norovirus ~~gii~~ 4]]	+	[[Category: Large Structures]]
-	[[Category: Amblard, F]]	+	[[Category: Norovirus GII 4]]
-	[[Category: Brunzelle~~, J S~~]]	+	[[Category: Amblard F]]
-	[[Category: Hackett, J]]	+	[[Category: Brunzelle JS]]
-	[[Category: Kovari~~, I A~~]]	+	[[Category: Hackett J]]
-	[[Category: Kovari~~, L C~~]]	+	[[Category: Kovari IA]]
-	[[Category: Kuiper~~, B D~~]]	+	[[Category: Kovari LC]]
-	[[Category: Muzzarelli~~, K M~~]]	+	[[Category: Kuiper BD]]
-	[[Category: Schinazi~~, R F~~]]	+	[[Category: Muzzarelli KM]]
-	[[Category: Spellmon~~, N S~~]]	+	[[Category: Schinazi RF]]
-	[[Category: Yang, Z]]	+	[[Category: Spellmon NS]]
-	~~[[Category: 3c-like protease]]~~	+	[[Category: Yang Z]]
-	~~[[Category: Gii 4]]~~	+
-	~~[[Category: Minerva]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Viral protease]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 12:28, 16 January 2019

OCA — Wed, 16 Jan 2019 12:28:46 GMT

←Older revision		Revision as of 12:28, 16 January 2019
Line 3:		Line 3:
	<StructureSection load='6b6i' size='340' side='right' caption='[[6b6i]], [[Resolution\|resolution]] 2.44Å' scene=''>		<StructureSection load='6b6i' size='340' side='right' caption='[[6b6i]], [[Resolution\|resolution]] 2.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b6i]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B6I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b6i]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Norovirus_gii.4 Norovirus gii.4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B6I FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b6i OCA], [http://pdbe.org/6b6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b6i RCSB], [http://www.ebi.ac.uk/pdbsum/6b6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b6i ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b6i OCA], [http://pdbe.org/6b6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b6i RCSB], [http://www.ebi.ac.uk/pdbsum/6b6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b6i ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/A0A076EGG6_9CALI A0A076EGG6_9CALI]] 3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave polyadenylate-binding protein thereby inhibiting cellular translation.[PROSITE-ProRule:PRU00870]		[[http://www.uniprot.org/uniprot/A0A076EGG6_9CALI A0A076EGG6_9CALI]] 3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave polyadenylate-binding protein thereby inhibiting cellular translation.[PROSITE-ProRule:PRU00870]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Norovirus is the leading cause of acute gastroenteritis worldwide with a yearly reported 700 million cases driving a $60 billion global socioeconomic burden. With no FDA approved therapeutics and the chance for severe chronic infection and life-threatening complications, researchers have identified the protease as a potential target. However, drug development has focused on the norovirus GI.1 strain despite its accounting for less than 5% of all outbreaks. Our lab aims to change focus for norovirus drug design from GI.1 to the highly infective GII.4; responsible for more than 50% of all outbreaks worldwide. With the first published crystal structure of the norovirus GII.4 protease, we have identified several significant differences in the structure and active site that have hindered development of a potent inhibitor targeting the norovirus GII.4 protease. With these new insights, we have begun designing compounds that demonstrate increased inhibition of the clinically most relevant norovirus GII.4 strain.
		+
		+	Structural and antiviral studies of the human norovirus GII.4 protease.,Muzzarelli KM, Kuiper BD, Spellmon N, Brunzelle JS, Hackett J, Amblard F, Zhou S, Liu P, Kovari IA, Yang Z, Schinazi RF, Kovari LC Biochemistry. 2019 Jan 3. doi: 10.1021/acs.biochem.8b01063. PMID:30605321<ref>PMID:30605321</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6b6i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Norovirus gii 4]]
	[[Category: Amblard, F]]		[[Category: Amblard, F]]
	[[Category: Brunzelle, J S]]		[[Category: Brunzelle, J S]]

OCA at 07:44, 3 October 2018

OCA — Wed, 03 Oct 2018 07:44:45 GMT

←Older revision		Revision as of 07:44, 3 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6b6i ~~is ON HOLD until Paper Publication~~	+	==2.4A resolution structure of human Norovirus GII.4 protease==
-		+	<StructureSection load='6b6i' size='340' side='right' caption='[[6b6i]], [[Resolution\|resolution]] 2.44Å' scene=''>
-	~~Authors~~: ~~Muzzarelli, K~~.M.~~, Kuiper, B~~.D.~~, Spellmon, N~~.S.~~, Hackett, J~~.~~, Brunzelle, J~~.S.~~, Kovari, I~~.A.~~, Amblard, F~~.~~, Yang, Z~~., ~~Schinazi, R~~.F., ~~Kovari~~, L.C.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6b6i]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B6I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B6I FirstGlance]. <br>
-	~~Description~~: 2.~~4A resolution structure of human Norovirus GII~~.~~4 protease~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b6i OCA], [http://pdbe.org/6b6i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b6i RCSB], [http://www.ebi.ac.uk/pdbsum/6b6i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b6i ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures]~~]	+	</table>
-	[[~~Category~~: ~~Kovari, I~~.A]]	+	== Function ==
-	~~[[Category~~: ~~Spellmon~~, N.S]]	+	[[http://www.uniprot.org/uniprot/A0A076EGG6_9CALI A0A076EGG6_9CALI]] 3C-like protease processes the polyprotein: 3CLpro-RdRp is first released by autocleavage, then all other proteins are cleaved. May cleave polyadenylate-binding protein thereby inhibiting cellular translation.[PROSITE-ProRule:PRU00870]
		+	__TOC__
		+	</StructureSection>
	[[Category: Amblard, F]]		[[Category: Amblard, F]]
		+	[[Category: Brunzelle, J S]]
		+	[[Category: Hackett, J]]
		+	[[Category: Kovari, I A]]
		+	[[Category: Kovari, L C]]
		+	[[Category: Kuiper, B D]]
		+	[[Category: Muzzarelli, K M]]
		+	[[Category: Schinazi, R F]]
		+	[[Category: Spellmon, N S]]
	[[Category: Yang, Z]]		[[Category: Yang, Z]]
-	[[Category: ~~Schinazi, R.F~~]]	+	[[Category: 3c-like protease]]
-	[[Category: ~~Muzzarelli, K.M~~]]	+	[[Category: Gii 4]]
-	[[Category: ~~Brunzelle, J.S~~]]	+	[[Category: Minerva]]
-	[[Category: ~~Hackett, J~~]]	+	[[Category: Protease]]
-	[[Category: ~~Kovari, L.C~~]]	+	[[Category: Viral protease]]
-	[[Category: ~~Kuiper, B.D~~]]	+	[[Category: Viral protein]]

OCA at 04:51, 1 November 2017

OCA — Wed, 01 Nov 2017 04:51:41 GMT

←Older revision		Revision as of 04:51, 1 November 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6b6i is ON HOLD	+	The entry 6b6i is ON HOLD until Paper Publication

	Authors: Muzzarelli, K.M., Kuiper, B.D., Spellmon, N.S., Hackett, J., Brunzelle, J.S., Kovari, I.A., Amblard, F., Yang, Z., Schinazi, R.F., Kovari, L.C.		Authors: Muzzarelli, K.M., Kuiper, B.D., Spellmon, N.S., Hackett, J., Brunzelle, J.S., Kovari, I.A., Amblard, F., Yang, Z., Schinazi, R.F., Kovari, L.C.

OCA: Protected "6b6i" [edit=sysop:move=sysop]

OCA — Wed, 11 Oct 2017 05:31:59 GMT

Protected "6b6i" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:31, 11 October 2017

OCA: New page: '''Unreleased structure''' The entry 6b6i is ON HOLD Authors: Muzzarelli, K.M., Kuiper, B.D., Spellmon, N.S., Hackett, J., Brunzelle, J.S., Kovari, I.A., Amblard, F., Yang, Z., Schinazi...

OCA — Wed, 11 Oct 2017 05:31:58 GMT

New page: '''Unreleased structure''' The entry 6b6i is ON HOLD Authors: Muzzarelli, K.M., Kuiper, B.D., Spellmon, N.S., Hackett, J., Brunzelle, J.S., Kovari, I.A., Amblard, F., Yang, Z., Schinazi...

New page

'''Unreleased structure'''

The entry 6b6i is ON HOLD

Authors: Muzzarelli, K.M., Kuiper, B.D., Spellmon, N.S., Hackett, J., Brunzelle, J.S., Kovari, I.A., Amblard, F., Yang, Z., Schinazi, R.F., Kovari, L.C.

Description: 2.4A resolution structure of human Norovirus GII.4 protease
[[Category: Unreleased Structures]]
[[Category: Kovari, I.A]]
[[Category: Spellmon, N.S]]
[[Category: Amblard, F]]
[[Category: Yang, Z]]
[[Category: Schinazi, R.F]]
[[Category: Muzzarelli, K.M]]
[[Category: Brunzelle, J.S]]
[[Category: Hackett, J]]
[[Category: Kovari, L.C]]
[[Category: Kuiper, B.D]]