6bc7 - Revision history

OCA at 14:38, 4 October 2023

OCA — Wed, 04 Oct 2023 14:38:12 GMT

←Older revision		Revision as of 14:38, 4 October 2023
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	==Cryo X-ray structure of sisomicin bound AAC-VIa==		==Cryo X-ray structure of sisomicin bound AAC-VIa==
-	<StructureSection load='6bc7' size='340' side='right' caption='[[6bc7]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='6bc7' size='340' side='right'caption='[[6bc7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bc7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter~~ cloacae~~" (jordan 1890) bergey et al. 1923~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BC7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BC7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bc7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BC7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BC7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SIS:(1S,2S,3R,4S,6R)-4,6-DIAMINO-3-{[(2S,3R)-3-AMINO-6-(AMINOMETHYL)-3,4-DIHYDRO-2H-PYRAN-2-YL]OXY}-2-HYDROXYCYCLOHEXYL+3-DEOXY-4-C-METHYL-3-(METHYLAMINO)-BETA-L-ARABINOPYRANOSIDE'>SIS</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac 3-VI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 "Aerobacter cloacae" (Jordan 1890) Bergey et al. 1923])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SIS:(1S,2S,3R,4S,6R)-4,6-DIAMINO-3-{[(2S,3R)-3-AMINO-6-(AMINOMETHYL)-3,4-DIHYDRO-2H-PYRAN-2-YL]OXY}-2-HYDROXYCYCLOHEXYL+3-DEOXY-4-C-METHYL-3-(METHYLAMINO)-BETA-L-ARABINOPYRANOSIDE'>SIS</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bc7 OCA], [~~http~~://pdbe.org/6bc7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bc7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bc7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bc7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bc7 OCA], [https://pdbe.org/6bc7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bc7 RCSB], [https://www.ebi.ac.uk/pdbsum/6bc7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bc7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q47030_ENTCL Q47030_ENTCL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cuneo, M J~~]]	+	[[Category: Enterobacter cloacae]]
-	[[Category: ~~Kumar, P~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Acetyltransferase~~]]	+	[[Category: Cuneo MJ]]
-	[[Category: ~~Sisomicin]]~~	+	[[Category: Kumar P]]
-	~~[[Category: Transferase-antibiotic complex~~]]	+

OCA at 07:49, 12 September 2018

OCA — Wed, 12 Sep 2018 07:49:46 GMT

←Older revision		Revision as of 07:49, 12 September 2018
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	<StructureSection load='6bc7' size='340' side='right' caption='[[6bc7]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='6bc7' size='340' side='right' caption='[[6bc7]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bc7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BC7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BC7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bc7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter cloacae" (jordan 1890) bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BC7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BC7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SIS:(1S,2S,3R,4S,6R)-4,6-DIAMINO-3-{[(2S,3R)-3-AMINO-6-(AMINOMETHYL)-3,4-DIHYDRO-2H-PYRAN-2-YL]OXY}-2-HYDROXYCYCLOHEXYL+3-DEOXY-4-C-METHYL-3-(METHYLAMINO)-BETA-L-ARABINOPYRANOSIDE'>SIS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SIS:(1S,2S,3R,4S,6R)-4,6-DIAMINO-3-{[(2S,3R)-3-AMINO-6-(AMINOMETHYL)-3,4-DIHYDRO-2H-PYRAN-2-YL]OXY}-2-HYDROXYCYCLOHEXYL+3-DEOXY-4-C-METHYL-3-(METHYLAMINO)-BETA-L-ARABINOPYRANOSIDE'>SIS</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac 3-VI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 "Aerobacter cloacae" (Jordan 1890) Bergey et al. 1923])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bc7 OCA], [http://pdbe.org/6bc7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bc7 RCSB], [http://www.ebi.ac.uk/pdbsum/6bc7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bc7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bc7 OCA], [http://pdbe.org/6bc7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bc7 RCSB], [http://www.ebi.ac.uk/pdbsum/6bc7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bc7 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	One group of enzymes that confer resistance to aminoglycoside antibiotics through covalent modification belongs to the GCN5-related N-acetyltransferase (GNAT) superfamily. We show how a unique GNAT subfamily member uses a previously unidentified noncanonical catalytic triad, consisting of a glutamic acid, a histidine, and the antibiotic substrate itself, which acts as a nucleophile and attacks the acetyl donor molecule. Neutron diffraction studies allow for unambiguous identification of a low-barrier hydrogen bond, predicted in canonical catalytic triads to increase basicity of the histidine. This work highlights the role of this unique catalytic triad in mediating antibiotic resistance while providing new insights into the design of the next generation of aminoglycosides.
		+
		+	A low-barrier hydrogen bond mediates antibiotic resistance in a noncanonical catalytic triad.,Kumar P, Serpersu EH, Cuneo MJ Sci Adv. 2018 Apr 4;4(4):eaas8667. doi: 10.1126/sciadv.aas8667. eCollection 2018 , Apr. PMID:29632894<ref>PMID:29632894</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6bc7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:31, 28 February 2018

OCA — Wed, 28 Feb 2018 06:31:49 GMT

←Older revision		Revision as of 06:31, 28 February 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6bc7 is ON HOLD~~	+	==Cryo X-ray structure of sisomicin bound AAC-VIa==
-		+	<StructureSection load='6bc7' size='340' side='right' caption='[[6bc7]], [[Resolution\|resolution]] 1.80Å' scene=''>
-	~~Authors: Cuneo, M.J., Kumar, P.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6bc7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BC7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BC7 FirstGlance]. <br>
-	~~Description:~~ Cryo X-ray structure of sisomicin bound AAC-VIa	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=SIS:(1S,2S,3R,4S,6R)-4,6-DIAMINO-3-{[(2S,3R)-3-AMINO-6-(AMINOMETHYL)-3,4-DIHYDRO-2H-PYRAN-2-YL]OXY}-2-HYDROXYCYCLOHEXYL+3-DEOXY-4-C-METHYL-3-(METHYLAMINO)-BETA-L-ARABINOPYRANOSIDE'>SIS</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bc7 OCA], [http://pdbe.org/6bc7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bc7 RCSB], [http://www.ebi.ac.uk/pdbsum/6bc7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bc7 ProSAT]</span></td></tr>
-	[[Category: Cuneo, M.J]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cuneo, M J]]
	[[Category: Kumar, P]]		[[Category: Kumar, P]]
		+	[[Category: Acetyltransferase]]
		+	[[Category: Sisomicin]]
		+	[[Category: Transferase-antibiotic complex]]

OCA: Protected "6bc7" [edit=sysop:move=sysop]

OCA — Wed, 01 Nov 2017 04:54:15 GMT

Protected "6bc7" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:54, 1 November 2017

OCA: New page: '''Unreleased structure''' The entry 6bc7 is ON HOLD Authors: Cuneo, M.J., Kumar, P. Description: Cryo X-ray structure of sisomicin bound AAC-VIa Category: Unreleased Structures [[...

OCA — Wed, 01 Nov 2017 04:54:13 GMT

New page: '''Unreleased structure''' The entry 6bc7 is ON HOLD Authors: Cuneo, M.J., Kumar, P. Description: Cryo X-ray structure of sisomicin bound AAC-VIa Category: Unreleased Structures [[...

New page

'''Unreleased structure'''

The entry 6bc7 is ON HOLD

Authors: Cuneo, M.J., Kumar, P.

Description: Cryo X-ray structure of sisomicin bound AAC-VIa
[[Category: Unreleased Structures]]
[[Category: Cuneo, M.J]]
[[Category: Kumar, P]]