OCA at 14:39, 4 October 2023

OCA — Wed, 04 Oct 2023 14:39:34 GMT

←Older revision		Revision as of 14:39, 4 October 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 6bfh is ~~ON HOLD~~	+	==Structure of the kanamycin complex of aminoglycoside acetyltransferase AAC(6')-Im==
		+	<StructureSection load='6bfh' size='340' side='right'caption='[[6bfh]], [[Resolution\|resolution]] 1.95Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6bfh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BFH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BFH FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.95Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=KAN:KANAMYCIN+A'>KAN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bfh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bfh OCA], [https://pdbe.org/6bfh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bfh RCSB], [https://www.ebi.ac.uk/pdbsum/6bfh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bfh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q93ET8_ECOLX Q93ET8_ECOLX]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aminoglycoside 6'-acetyltransferase-Im (AAC(6')-Im) is the closest monofunctional homolog of the AAC(6')-Ie acetyltransferase of the bifunctional enzyme AAC(6')-Ie/APH(2")-Ia. The AAC(6')-Im acetyltransferase confers 4- to 64-fold higher MICs to 4,6-disubstituted aminoglycosides and the 4,5-disubstituted aminoglycoside neomycin than AAC(6')-Ie, yet unlike AAC(6')-Ie, the AAC(6')-Im enzyme does not confer resistance to the atypical aminoglycoside fortimicin. The structure of the kanamycin A complex of AAC(6')-Im shows that the substrate binds in a shallow positively-charged pocket, with the N6' amino group positioned appropriately for an efficient nucleophilic attack on an acetyl-CoA cofactor. The AAC(6')-Ie enzyme binds kanamycin A in a sufficiently different manner to position the N6' group less efficiently, thereby reducing the activity of this enzyme towards the 4,6-disubstituted aminoglycosides. Conversely, docking studies with fortimicin in both acetyltransferases suggest that the atypical aminoglycoside might bind less productively in AAC(6')-Im, thus explaining the lack of resistance to this molecule.

-	~~Authors:~~ Smith, ~~C.A.~~, ~~Vakulenko~~, S.B.	+	Aminoglycoside resistance profile and structural architecture of the aminoglycoside acetyltransferase AAC(6')-Im.,Smith CA, Bhattacharya M, Toth M, Stewart NK, Vakulenko SB Microb Cell. 2017 Nov 9;4(12):402-410. doi: 10.15698/mic2017.12.602. PMID:29234669<ref>PMID:29234669</ref>

-	~~Description: Structure~~ of the ~~kanamycin complex~~ of ~~aminoglycoside acetyltransferase AAC(6')~~-Im	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Vakulenko, S.B~~]]	+	<div class="pdbe-citations 6bfh" style="background-color:#fffaf0;"></div>
-	[[Category: Smith~~, C.A~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
		+	[[Category: Smith CA]]
		+	[[Category: Vakulenko SB]]

OCA: Protected "6bfh" [edit=sysop:move=sysop]

OCA — Wed, 01 Nov 2017 05:02:17 GMT

Protected "6bfh" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:02, 1 November 2017

OCA: New page: '''Unreleased structure''' The entry 6bfh is ON HOLD Authors: Smith, C.A., Vakulenko, S.B. Description: Structure of the kanamycin complex of aminoglycoside acetyltransferase AAC(6')-I...

OCA — Wed, 01 Nov 2017 05:02:16 GMT

New page: '''Unreleased structure''' The entry 6bfh is ON HOLD Authors: Smith, C.A., Vakulenko, S.B. Description: Structure of the kanamycin complex of aminoglycoside acetyltransferase AAC(6')-I...

New page

'''Unreleased structure'''

The entry 6bfh is ON HOLD

Authors: Smith, C.A., Vakulenko, S.B.

Description: Structure of the kanamycin complex of aminoglycoside acetyltransferase AAC(6')-Im
[[Category: Unreleased Structures]]
[[Category: Vakulenko, S.B]]
[[Category: Smith, C.A]]

6bfh - Revision history

OCA at 14:39, 4 October 2023

OCA: Protected "6bfh" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6bfh is ON HOLD Authors: Smith, C.A., Vakulenko, S.B. Description: Structure of the kanamycin complex of aminoglycoside acetyltransferase AAC(6')-I...