6bi0 - Revision history

OCA at 14:41, 4 October 2023

OCA — Wed, 04 Oct 2023 14:41:27 GMT

←Older revision		Revision as of 14:41, 4 October 2023
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	<StructureSection load='6bi0' size='340' side='right'caption='[[6bi0]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='6bi0' size='340' side='right'caption='[[6bi0]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bi0]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BI0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6BI0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bi0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BI0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BI0 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=EDO:1~~,2~~-ETHANEDIOL'>EDO</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.057Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[6bhz\|6bhz]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6bi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bi0 OCA], [~~http~~://pdbe.org/6bi0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bi0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bi0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bi0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bi0 OCA], [https://pdbe.org/6bi0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bi0 RCSB], [https://www.ebi.ac.uk/pdbsum/6bi0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bi0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: DiDonato, M]]	+	[[Category: DiDonato M]]
-	[[Category: Spraggon, G]]	+	[[Category: Spraggon G]]
-	~~[[Category: Fab]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Immunoglobulin]]~~	+
-	~~[[Category: Mutant~~]]	+

OCA at 08:49, 11 November 2020

OCA — Wed, 11 Nov 2020 08:49:59 GMT

←Older revision		Revision as of 08:49, 11 November 2020
Line 1:		Line 1:

	==Trastuzumab Fab N158A, D185A, K190A (Light Chain) Triple Mutant.==		==Trastuzumab Fab N158A, D185A, K190A (Light Chain) Triple Mutant.==
-	<StructureSection load='6bi0' size='340' side='right' caption='[[6bi0]], [[Resolution\|resolution]] 2.06Å' scene=''>	+	<StructureSection load='6bi0' size='340' side='right'caption='[[6bi0]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bi0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BI0 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BI0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bi0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BI0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6BI0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6bhz\|6bhz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6bhz\|6bhz]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bi0 OCA], [http://pdbe.org/6bi0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bi0 RCSB], [http://www.ebi.ac.uk/pdbsum/6bi0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bi0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6bi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bi0 OCA], [http://pdbe.org/6bi0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bi0 RCSB], [http://www.ebi.ac.uk/pdbsum/6bi0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bi0 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: DiDonato, M]]		[[Category: DiDonato, M]]
	[[Category: Spraggon, G]]		[[Category: Spraggon, G]]

OCA at 07:11, 28 March 2018

OCA — Wed, 28 Mar 2018 07:11:35 GMT

←Older revision		Revision as of 07:11, 28 March 2018
Line 3:		Line 3:
	<StructureSection load='6bi0' size='340' side='right' caption='[[6bi0]], [[Resolution\|resolution]] 2.06Å' scene=''>		<StructureSection load='6bi0' size='340' side='right' caption='[[6bi0]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bi0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BI0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bi0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BI0 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6bhz\|6bhz]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6bhz\|6bhz]]</td></tr>
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: DiDonato, M]]		[[Category: DiDonato, M]]
	[[Category: Spraggon, G]]		[[Category: Spraggon, G]]

OCA at 06:33, 15 February 2018

OCA — Thu, 15 Feb 2018 06:33:29 GMT

←Older revision		Revision as of 06:33, 15 February 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6bi0 is ~~ON HOLD until Paper~~ Publication	+	==Trastuzumab Fab N158A, D185A, K190A (Light Chain) Triple Mutant.==
		+	<StructureSection load='6bi0' size='340' side='right' caption='[[6bi0]], [[Resolution\|resolution]] 2.06Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6bi0]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BI0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BI0 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6bhz\|6bhz]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bi0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bi0 OCA], [http://pdbe.org/6bi0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bi0 RCSB], [http://www.ebi.ac.uk/pdbsum/6bi0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bi0 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Activated esters are widely used to label proteins at lysine sidechains and N-termini. These reagents are useful for labeling virtually any protein, but robust reactivity toward primary amines generally precludes site-selective modification. In a unique case, fluoro-phenyl esters are shown to preferentially label human kappa antibodies at a single lysine (K188) within the light chain constant domain. Neighboring residues H189 and D151 contribute to the accelerated rate of labeling at K188 relative to ~40 other lysine sites. Enriched K188 labeling can be enhanced from 50-70% to >95% by any of these approaches: lowering reaction temperature, applying flow chemistry, or mutagenesis of specific residues in the surrounding protein environment. Our results demonstrate that activated esters with fluoro-substituted aromatic leaving groups, including a fluoro-napthyl ester, can be generally useful reagents for site-selective lysine labeling of antibodies and other immunoglobulin-type proteins.

-	~~Authors: DiDonato~~, M., ~~Spraggon~~, G.	+	Tuning a Protein Labeling Reaction to Achieve Highly Site-Selective Lysine Conjugation.,Pham GH, Ou W, Bursulaya B, DiDonato M, Herath A, Jin Y, Hao X, Loren J, Spraggon G, Brock A, Uno T, Geierstanger BH, Cellitti S Chembiochem. 2018 Jan 31. doi: 10.1002/cbic.201700611. PMID:29388367<ref>PMID:29388367</ref>

-	~~Description: Trastuzumab Fab N158A~~, ~~D185A, K190A (Light Chain) Triple Mutant~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6bi0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: DiDonato, M]]
	[[Category: Spraggon, G]]		[[Category: Spraggon, G]]
-	[[Category: ~~Didonato, M~~]]	+	[[Category: Fab]]
		+	[[Category: Immune system]]
		+	[[Category: Immunoglobulin]]
		+	[[Category: Mutant]]

OCA at 06:21, 15 November 2017

OCA — Wed, 15 Nov 2017 06:21:28 GMT

←Older revision		Revision as of 06:21, 15 November 2017
Line 3:		Line 3:
	The entry 6bi0 is ON HOLD until Paper Publication		The entry 6bi0 is ON HOLD until Paper Publication

-	Authors: ~~Pham, G.H., Ou, W., Bursulaya, B.,~~ DiDonato, M., ~~Herath~~, ~~A., Jin, Y., Hao, X., Loren, J., Brock, A., Uno, T., Geierstanger, B.H., Cellitti, S.E~~.	+	Authors: DiDonato, M., Spraggon, G.

	Description: Trastuzumab Fab N158A, D185A, K190A (Light Chain) Triple Mutant.		Description: Trastuzumab Fab N158A, D185A, K190A (Light Chain) Triple Mutant.
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: ~~Geierstanger, B.H]]~~	+	[[Category: Spraggon, G]]
-	~~[[Category: Loren, J]]~~	+
-	~~[[Category: Ou, W]]~~	+
-	~~[[Category: Uno, T]]~~	+
-	~~[[Category: Pham~~, G~~.H]]~~	+
-	~~[[Category: Hao, X]]~~	+
-	~~[[Category: Herath, A]]~~	+
-	~~[[Category: Brock, A]]~~	+
-	~~[[Category: Jin, Y]]~~	+
-	~~[[Category: Bursulaya, B]]~~	+
-	~~[[Category: Cellitti, S.E~~]]	+
	[[Category: Didonato, M]]		[[Category: Didonato, M]]

OCA: Protected "6bi0" [edit=sysop:move=sysop]

OCA — Wed, 08 Nov 2017 06:37:24 GMT

Protected "6bi0" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:37, 8 November 2017

OCA: New page: '''Unreleased structure''' The entry 6bi0 is ON HOLD until Paper Publication Authors: Pham, G.H., Ou, W., Bursulaya, B., DiDonato, M., Herath, A., Jin, Y., Hao, X., Loren, J., Brock, A....

OCA — Wed, 08 Nov 2017 06:37:23 GMT

New page: '''Unreleased structure''' The entry 6bi0 is ON HOLD until Paper Publication Authors: Pham, G.H., Ou, W., Bursulaya, B., DiDonato, M., Herath, A., Jin, Y., Hao, X., Loren, J., Brock, A....

New page

'''Unreleased structure'''

The entry 6bi0 is ON HOLD until Paper Publication

Authors: Pham, G.H., Ou, W., Bursulaya, B., DiDonato, M., Herath, A., Jin, Y., Hao, X., Loren, J., Brock, A., Uno, T., Geierstanger, B.H., Cellitti, S.E.

Description: Trastuzumab Fab N158A, D185A, K190A (Light Chain) Triple Mutant.
[[Category: Unreleased Structures]]
[[Category: Geierstanger, B.H]]
[[Category: Loren, J]]
[[Category: Ou, W]]
[[Category: Uno, T]]
[[Category: Pham, G.H]]
[[Category: Hao, X]]
[[Category: Herath, A]]
[[Category: Brock, A]]
[[Category: Jin, Y]]
[[Category: Bursulaya, B]]
[[Category: Cellitti, S.E]]
[[Category: Didonato, M]]