6bjc - Revision history

OCA at 14:22, 13 March 2024

OCA — Wed, 13 Mar 2024 14:22:20 GMT

←Older revision		Revision as of 14:22, 13 March 2024
Line 3:		Line 3:
	<SX load='6bjc' size='340' side='right' viewer='molstar' caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='6bjc' size='340' side='right' viewer='molstar' caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~] and [~~http~~://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6BJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BJC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.3Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPX2, C20orf1, C20orf2, DIL2, HCA519 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bjc OCA], [~~http~~://pdbe.org/6bjc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bjc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bjc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bjc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bjc OCA], [https://pdbe.org/6bjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bjc RCSB], [https://www.ebi.ac.uk/pdbsum/6bjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bjc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TPX2_HUMAN TPX2_HUMAN]] Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.<ref>PMID:18663142</ref> <ref>PMID:19208764</ref> [[http://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.	+	[https://www.uniprot.org/uniprot/TPX2_HUMAN TPX2_HUMAN] Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.<ref>PMID:18663142</ref> <ref>PMID:19208764</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	During mitosis and meiosis, microtubule (MT) assembly is locally upregulated by the chromatin-dependent Ran-GTP pathway. One of its key targets is the MT-associated spindle assembly factor TPX2. The molecular mechanism of how TPX2 stimulates MT assembly remains unknown because structural information about the interaction of TPX2 with MTs is lacking. Here, we determine the cryo-electron microscopy structure of a central region of TPX2 bound to the MT surface. TPX2 uses two flexibly linked elements ('ridge' and 'wedge') in a novel interaction mode to simultaneously bind across longitudinal and lateral tubulin interfaces. These MT-interacting elements overlap with the binding site of importins on TPX2. Fluorescence microscopy-based in vitro reconstitution assays reveal that this interaction mode is critical for MT binding and facilitates MT nucleation. Together, our results suggest a molecular mechanism of how the Ran-GTP gradient can regulate TPX2-dependent MT formation.	+
-		+
-	~~Structural insight into TPX2-stimulated microtubule assembly.,Zhang R, Roostalu J, Surrey T, Nogales E Elife. 2017 Nov 9;6. pii: e30959. doi: 10.7554/eLife.30959. PMID:29120325<ref>PMID:29120325</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6bjc" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 26:		Line 17:
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
-	[[Category: Nogales, E]]	+	[[Category: Nogales E]]
-	[[Category: Zhang, R]]	+	[[Category: Zhang R]]
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Gangtp]]~~	+
-	~~[[Category: Mitosis]]~~	+
-	~~[[Category: Nucleation~~]]	+

OCA at 23:23, 10 April 2020

OCA — Fri, 10 Apr 2020 23:23:37 GMT

←Older revision		Revision as of 23:23, 10 April 2020
Line 3:		Line 3:
	<SX load='6bjc' size='340' side='right' viewer='molstar' caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='6bjc' size='340' side='right' viewer='molstar' caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6BJC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPX2, C20orf1, C20orf2, DIL2, HCA519 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPX2, C20orf1, C20orf2, DIL2, HCA519 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bjc OCA], [http://pdbe.org/6bjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bjc RCSB], [http://www.ebi.ac.uk/pdbsum/6bjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bjc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bjc OCA], [http://pdbe.org/6bjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bjc RCSB], [http://www.ebi.ac.uk/pdbsum/6bjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bjc ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Tubulin\|Tubulin]]	+	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:32, 6 March 2020

OCA — Fri, 06 Mar 2020 20:32:05 GMT

←Older revision		Revision as of 20:32, 6 March 2020
Line 1:		Line 1:

	==TPX2_mini decorated GMPCPP-microtubule==		==TPX2_mini decorated GMPCPP-microtubule==
-	<~~StructureSection~~ load='6bjc' size='340' side='right'caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<SX load='6bjc' size='340' side='right' viewer='molstar' caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BJC FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BJC FirstGlance]. <br>
Line 25:		Line 25:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 17:10, 20 November 2019

OCA — Wed, 20 Nov 2019 17:10:34 GMT

←Older revision		Revision as of 17:10, 20 November 2019
Line 1:		Line 1:

	==TPX2_mini decorated GMPCPP-microtubule==		==TPX2_mini decorated GMPCPP-microtubule==
-	<StructureSection load='6bjc' size='340' side='right' caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<StructureSection load='6bjc' size='340' side='right'caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BJC FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BJC FirstGlance]. <br>
Line 27:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Nogales, E]]		[[Category: Nogales, E]]

OCA at 08:40, 3 October 2018

OCA — Wed, 03 Oct 2018 08:40:55 GMT

←Older revision		Revision as of 08:40, 3 October 2018
Line 3:		Line 3:
	<StructureSection load='6bjc' size='340' side='right' caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='6bjc' size='340' side='right' caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BJC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TPX2, C20orf1, C20orf2, DIL2, HCA519 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bjc OCA], [http://pdbe.org/6bjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bjc RCSB], [http://www.ebi.ac.uk/pdbsum/6bjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bjc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bjc OCA], [http://pdbe.org/6bjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bjc RCSB], [http://www.ebi.ac.uk/pdbsum/6bjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bjc ProSAT]</span></td></tr>
	</table>		</table>
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6bjc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6bjc" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tubulin\|Tubulin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Sus scrofa]]		[[Category: Sus scrofa]]
	[[Category: Nogales, E]]		[[Category: Nogales, E]]

OCA at 07:48, 22 November 2017

OCA — Wed, 22 Nov 2017 07:48:44 GMT

←Older revision		Revision as of 07:48, 22 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6bjc is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==TPX2_mini decorated GMPCPP-microtubule==
		+	<StructureSection load='6bjc' size='340' side='right' caption='[[6bjc]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6bjc]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BJC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bjc OCA], [http://pdbe.org/6bjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bjc RCSB], [http://www.ebi.ac.uk/pdbsum/6bjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bjc ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/TPX2_HUMAN TPX2_HUMAN]] Spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.<ref>PMID:18663142</ref> <ref>PMID:19208764</ref> [[http://www.uniprot.org/uniprot/TBB_PIG TBB_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During mitosis and meiosis, microtubule (MT) assembly is locally upregulated by the chromatin-dependent Ran-GTP pathway. One of its key targets is the MT-associated spindle assembly factor TPX2. The molecular mechanism of how TPX2 stimulates MT assembly remains unknown because structural information about the interaction of TPX2 with MTs is lacking. Here, we determine the cryo-electron microscopy structure of a central region of TPX2 bound to the MT surface. TPX2 uses two flexibly linked elements ('ridge' and 'wedge') in a novel interaction mode to simultaneously bind across longitudinal and lateral tubulin interfaces. These MT-interacting elements overlap with the binding site of importins on TPX2. Fluorescence microscopy-based in vitro reconstitution assays reveal that this interaction mode is critical for MT binding and facilitates MT nucleation. Together, our results suggest a molecular mechanism of how the Ran-GTP gradient can regulate TPX2-dependent MT formation.

-	~~Authors~~:	+	Structural insight into TPX2-stimulated microtubule assembly.,Zhang R, Roostalu J, Surrey T, Nogales E Elife. 2017 Nov 9;6. pii: e30959. doi: 10.7554/eLife.30959. PMID:29120325<ref>PMID:29120325</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6bjc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Sus scrofa]]
		+	[[Category: Nogales, E]]
		+	[[Category: Zhang, R]]
		+	[[Category: Cell cycle]]
		+	[[Category: Gangtp]]
		+	[[Category: Mitosis]]
		+	[[Category: Nucleation]]

OCA: Protected "6bjc" [edit=sysop:move=sysop]

OCA — Wed, 15 Nov 2017 06:22:22 GMT

Protected "6bjc" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:22, 15 November 2017

OCA: New page: '''Unreleased structure''' The entry 6bjc is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Nov 2017 06:22:20 GMT

New page: '''Unreleased structure''' The entry 6bjc is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

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'''Unreleased structure'''

The entry 6bjc is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]