6bjx - Revision history

OCA at 14:43, 4 October 2023

OCA — Wed, 04 Oct 2023 14:43:48 GMT

←Older revision		Revision as of 14:43, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='6bjx' size='340' side='right'caption='[[6bjx]], [[Resolution\|resolution]] 3.14Å' scene=''>		<StructureSection load='6bjx' size='340' side='right'caption='[[6bjx]], [[Resolution\|resolution]] 3.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bjx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BJX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bjx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BJX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.14Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bjx OCA], [~~http~~://pdbe.org/6bjx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bjx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bjx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bjx ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bjx OCA], [https://pdbe.org/6bjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bjx RCSB], [https://www.ebi.ac.uk/pdbsum/6bjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bjx ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 18:		Line 19:

	==See Also==		==See Also==
-	*[[Ribozyme\|Ribozyme]]	+	*[[Ribozyme 3D structures\|Ribozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Shoffner, G M~~]]	+	[[Category: Tetrahymena thermophila]]
-	[[Category: ~~Ribozyme]]~~	+	[[Category: Shoffner GM]]
-	~~[[Category: Rna~~]]	+

OCA at 12:28, 17 July 2019

OCA — Wed, 17 Jul 2019 12:28:28 GMT

←Older revision		Revision as of 12:28, 17 July 2019
Line 1:		Line 1:

	==Group I self-splicing intron P4-P6 domain mutant U131A (with isopropanol soaking)==		==Group I self-splicing intron P4-P6 domain mutant U131A (with isopropanol soaking)==
-	<StructureSection load='6bjx' size='340' side='right' caption='[[6bjx]], [[Resolution\|resolution]] 3.14Å' scene=''>	+	<StructureSection load='6bjx' size='340' side='right'caption='[[6bjx]], [[Resolution\|resolution]] 3.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bjx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BJX FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bjx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BJX FirstGlance]. <br>
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Shoffner, G M]]		[[Category: Shoffner, G M]]
	[[Category: Ribozyme]]		[[Category: Ribozyme]]
	[[Category: Rna]]		[[Category: Rna]]

OCA at 20:16, 19 September 2018

OCA — Wed, 19 Sep 2018 20:16:03 GMT

←Older revision		Revision as of 20:16, 19 September 2018
Line 16:		Line 16:
	</div>		</div>
	<div class="pdbe-citations 6bjx" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6bjx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribozyme\|Ribozyme]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:11, 4 July 2018

OCA — Wed, 04 Jul 2018 07:11:22 GMT

←Older revision		Revision as of 07:11, 4 July 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6bjx is ~~ON HOLD until Paper~~ Publication	+	==Group I self-splicing intron P4-P6 domain mutant U131A (with isopropanol soaking)==
		+	<StructureSection load='6bjx' size='340' side='right' caption='[[6bjx]], [[Resolution\|resolution]] 3.14Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6bjx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BJX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BJX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bjx OCA], [http://pdbe.org/6bjx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bjx RCSB], [http://www.ebi.ac.uk/pdbsum/6bjx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bjx ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Crystallography is a major technique for determining large RNA structures. Obtaining diffraction-quality crystals has been the bottleneck. Although several RNA crystallization methods have been developed, the field strongly needs additional approaches. Here we invented an in crystallo selection strategy for identifying mutations that enhance a target RNA's crystallizability. The strategy includes constructing an RNA pool containing random mutations, obtaining crystals, and amplifying the sequences enriched by crystallization. We demonstrated a proof-of-principle application to the P4-P6 domain from the Tetrahymena ribozyme. We further determined the structures of four selected mutants. All four establish new crystal lattice contacts while maintaining the native structure. Three mutants achieve this by relocating bulges and one by making a helix more flexible. In crystallo selection provides opportunities to improve crystals of RNAs or RNA-ligand complexes. Our results also suggest that mutants may be rationally designed for crystallization by "walking" a bulge along the RNA chain.

-	~~Authors~~:	+	In Crystallo Selection to Establish New RNA Crystal Contacts.,Shoffner GM, Wang R, Podell E, Cech TR, Guo F Structure. 2018 May 28. pii: S0969-2126(18)30169-2. doi:, 10.1016/j.str.2018.05.005. PMID:29910185<ref>PMID:29910185</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6bjx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Shoffner, G M]]
		+	[[Category: Ribozyme]]
		+	[[Category: Rna]]

OCA at 03:57, 25 April 2018

OCA — Wed, 25 Apr 2018 03:57:37 GMT

←Older revision		Revision as of 03:57, 25 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6bjx is ON HOLD	+	The entry 6bjx is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6bjx" [edit=sysop:move=sysop]

OCA — Wed, 15 Nov 2017 06:23:48 GMT

Protected "6bjx" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:23, 15 November 2017

OCA: New page: '''Unreleased structure''' The entry 6bjx is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Nov 2017 06:23:47 GMT

New page: '''Unreleased structure''' The entry 6bjx is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6bjx is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]