6bk1 - Revision history

OCA at 14:43, 4 October 2023

OCA — Wed, 04 Oct 2023 14:43:57 GMT

←Older revision		Revision as of 14:43, 4 October 2023
Line 1:		Line 1:

	==Crystal structure of Os79 T291V from O. sativa in complex with UDP.==		==Crystal structure of Os79 T291V from O. sativa in complex with UDP.==
-	<StructureSection load='6bk1' size='340' side='right' caption='[[6bk1]], [[Resolution\|resolution]] 1.58Å' scene=''>	+	<StructureSection load='6bk1' size='340' side='right'caption='[[6bk1]], [[Resolution\|resolution]] 1.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bk1]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Japanese_rice Japanese rice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BK1 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BK1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bk1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BK1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BK1 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=UDP:URIDINE~~-~~5-DIPHOSPHATE'>UDP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.58Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~UGT79, OS79, Os04g0206600, LOC_Os04g12970, OsJ_13826, OSJNBa0052O21.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=5~~&id=39947 Japanese rice])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bk1 OCA], [~~http~~://pdbe.org/6bk1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bk1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bk1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bk1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bk1 OCA], [https://pdbe.org/6bk1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bk1 RCSB], [https://www.ebi.ac.uk/pdbsum/6bk1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bk1 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/UGT79_ORYSJ UGT79_ORYSJ]] Involved in the detoxification of the Fusarium mycotoxin deoxynivalenol by the transfer of glucose from UDP-D-glucose to the hydroxyl group at C-3, forming deoxynivalenol-3-O-beta-D-glucoside.<ref>PMID:26197338</ref> <ref>PMID:27715009</ref>	+	[https://www.uniprot.org/uniprot/UGT79_ORYSJ UGT79_ORYSJ] Involved in the detoxification of the Fusarium mycotoxin deoxynivalenol by the transfer of glucose from UDP-D-glucose to the hydroxyl group at C-3, forming deoxynivalenol-3-O-beta-D-glucoside.<ref>PMID:26197338</ref> <ref>PMID:27715009</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6bk1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6bk1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Japanese rice~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Gabardi, K~~]]	+	[[Category: Oryza sativa Japonica Group]]
-	[[Category: ~~Rayment, I]]~~	+	[[Category: Gabardi K]]
-	~~[[Category: Wetterhorn,~~ K]]	+	[[Category: Rayment I]]
-	[[Category: ~~Transferase~~]]	+	[[Category: Wetterhorn K]]
-	[[Category: ~~Trichothecene]]~~	+
-	~~[[Category: Udp-glucosyltransferase~~]]	+

OCA at 06:03, 3 January 2018

OCA — Wed, 03 Jan 2018 06:03:57 GMT

←Older revision		Revision as of 06:03, 3 January 2018
Line 3:		Line 3:
	<StructureSection load='6bk1' size='340' side='right' caption='[[6bk1]], [[Resolution\|resolution]] 1.58Å' scene=''>		<StructureSection load='6bk1' size='340' side='right' caption='[[6bk1]], [[Resolution\|resolution]] 1.58Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bk1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BK1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bk1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_rice Japanese rice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BK1 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">UGT79, OS79, Os04g0206600, LOC_Os04g12970, OsJ_13826, OSJNBa0052O21.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=39947 Japanese rice])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bk1 OCA], [http://pdbe.org/6bk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bk1 RCSB], [http://www.ebi.ac.uk/pdbsum/6bk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bk1 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bk1 OCA], [http://pdbe.org/6bk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bk1 RCSB], [http://www.ebi.ac.uk/pdbsum/6bk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bk1 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Japanese rice]]
	[[Category: Gabardi, K]]		[[Category: Gabardi, K]]
	[[Category: Rayment, I]]		[[Category: Rayment, I]]

OCA at 06:16, 29 November 2017

OCA — Wed, 29 Nov 2017 06:16:04 GMT

←Older revision		Revision as of 06:16, 29 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6bk1 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of Os79 T291V from O. sativa in complex with UDP.==
		+	<StructureSection load='6bk1' size='340' side='right' caption='[[6bk1]], [[Resolution\|resolution]] 1.58Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6bk1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BK1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BK1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bk1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bk1 OCA], [http://pdbe.org/6bk1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bk1 RCSB], [http://www.ebi.ac.uk/pdbsum/6bk1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bk1 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/UGT79_ORYSJ UGT79_ORYSJ]] Involved in the detoxification of the Fusarium mycotoxin deoxynivalenol by the transfer of glucose from UDP-D-glucose to the hydroxyl group at C-3, forming deoxynivalenol-3-O-beta-D-glucoside.<ref>PMID:26197338</ref> <ref>PMID:27715009</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Family 1 UDP-glycosyltransferases in plants (UGTs) primarily form glucose conjugates of small molecules and, besides other functions, play a role in detoxification of xenobiotics. Indeed, overexpression of a barley UGT in wheat has been shown to control Fusarium head blight, where this is a plant disease of global significance that leads to reduced crop yields and contamination with trichothecene mycotoxins such as deoxynivalenol (DON), T-2 toxin and many other structural variants. The UGT Os79 from rice has emerged as a promising candidate for inactivation of mycotoxins on account of its ability to glycosylate DON, nivalenol and hydrolyzed T-2 toxin (HT-2). However, Os79 is unable to modify T-2 toxin (T-2), produced by pathogens such as F. sporotrichioides and F. langsethii. Activity towards T-2 is desirable because it would allow a single UGT to inactivate co-occurring mycotoxins. Here, the structure of Os79 in complex with the products, UDP and deoxynivalenol-3-O-glucoside is reported together with a kinetic analysis of a broad range of trichothecene mycotoxins. Residues associated with the trichothecene binding pocket were examined by site-directed mutagenesis which revealed that trichothecenes substituted on the C4 position, which are not glycosylated by WT Os79, can be accommodated in the binding pocket by increasing its volume. The triple mutation H122A/L123A/Q202L, which increases the volume of the active site and attenuates polar contacts, led to strong and equivalent activity towards trichothecenes with C4 acetyl groups. This mutant enzyme provides the broad specificity required to control multiple toxins produced by different Fusarium species and chemotypes.

-	~~Authors:~~ Wetterhorn, K., ~~Gabardi~~, K., ~~Rayment~~, I.	+	Determinants and Expansion of Specificity in a Trichothecene UDP-glucosyltransferase from Oryza sativa.,Wetterhorn KM, Gabardi K, Michlmayr H, Malachova A, Busman M, McCormick SP, Berthiller F, Adam G, Rayment I Biochemistry. 2017 Nov 15. doi: 10.1021/acs.biochem.7b01007. PMID:29140092<ref>PMID:29140092</ref>

-	~~Description: Crystal structure~~ of ~~Os79 T291V from O~~. ~~sativa in complex with UDP~~.	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Rayment, I]]~~	+	<div class="pdbe-citations 6bk1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Gabardi, K]]		[[Category: Gabardi, K]]
		+	[[Category: Rayment, I]]
	[[Category: Wetterhorn, K]]		[[Category: Wetterhorn, K]]
		+	[[Category: Transferase]]
		+	[[Category: Trichothecene]]
		+	[[Category: Udp-glucosyltransferase]]

OCA: Protected "6bk1" [edit=sysop:move=sysop]

OCA — Wed, 15 Nov 2017 06:24:05 GMT

Protected "6bk1" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:24, 15 November 2017

OCA: New page: '''Unreleased structure''' The entry 6bk1 is ON HOLD until Paper Publication Authors: Wetterhorn, K., Gabardi, K., Rayment, I. Description: Crystal structure of Os79 T291V from O. sati...

OCA — Wed, 15 Nov 2017 06:24:04 GMT

New page: '''Unreleased structure''' The entry 6bk1 is ON HOLD until Paper Publication Authors: Wetterhorn, K., Gabardi, K., Rayment, I. Description: Crystal structure of Os79 T291V from O. sati...

New page

'''Unreleased structure'''

The entry 6bk1 is ON HOLD until Paper Publication

Authors: Wetterhorn, K., Gabardi, K., Rayment, I.

Description: Crystal structure of Os79 T291V from O. sativa in complex with UDP.
[[Category: Unreleased Structures]]
[[Category: Rayment, I]]
[[Category: Gabardi, K]]
[[Category: Wetterhorn, K]]