6bld - Revision history

OCA at 14:45, 4 October 2023

OCA — Wed, 04 Oct 2023 14:45:03 GMT

←Older revision		Revision as of 14:45, 4 October 2023
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	<StructureSection load='6bld' size='340' side='right'caption='[[6bld]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6bld' size='340' side='right'caption='[[6bld]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bld]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mycmm Mycmm~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BLD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BLD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bld]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_marinum_M Mycobacterium marinum M]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BLD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BLD FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DXJ:(3E,5E)-6,10-dimethylundeca-3,5,9-trien-2-one'>DXJ</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.997Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cyp268A2, MMAR_3761 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216594 MYCMM])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DXJ:(3E,5E)-6,10-dimethylundeca-3,5,9-trien-2-one'>DXJ</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bld OCA], [~~http~~://pdbe.org/6bld PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bld RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bld PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bld ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bld OCA], [https://pdbe.org/6bld PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bld RCSB], [https://www.ebi.ac.uk/pdbsum/6bld PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bld ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/B2HMF7_MYCMM B2HMF7_MYCMM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Mycmm~~]]	+	[[Category: Mycobacterium marinum M]]
-	[[Category: Bell~~, S G~~]]	+	[[Category: Bell SG]]
-	[[Category: Bruning~~, J B~~]]	+	[[Category: Bruning JB]]
-	[[Category: Child~~, S A]]~~	+	[[Category: Child SA]]
-	~~[[Category: Cytochrome p450]]~~	+
-	~~[[Category: Heme protein]]~~	+
-	~~[[Category: Monooxygenase]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 10:21, 1 January 2020

OCA — Wed, 01 Jan 2020 10:21:55 GMT

←Older revision		Revision as of 10:21, 1 January 2020
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	==Mycobacterium marinum cytochrome P450 CYP268A2 in complex with pseudoionone==		==Mycobacterium marinum cytochrome P450 CYP268A2 in complex with pseudoionone==
-	<StructureSection load='6bld' size='340' side='right' caption='[[6bld]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='6bld' size='340' side='right'caption='[[6bld]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycmm Mycmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BLD FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycmm Mycmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BLD FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 6bld" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6bld" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Mycmm]]		[[Category: Mycmm]]
	[[Category: Bell, S G]]		[[Category: Bell, S G]]

OCA at 08:27, 22 February 2018

OCA — Thu, 22 Feb 2018 08:27:17 GMT

←Older revision		Revision as of 08:27, 22 February 2018
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	<StructureSection load='6bld' size='340' side='right' caption='[[6bld]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6bld' size='340' side='right' caption='[[6bld]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bld]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BLD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bld]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycmm Mycmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BLD FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DXJ:(3E,5E)-6,10-dimethylundeca-3,5,9-trien-2-one'>DXJ</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DXJ:(3E,5E)-6,10-dimethylundeca-3,5,9-trien-2-one'>DXJ</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cyp268A2, MMAR_3761 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216594 MYCMM])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bld OCA], [http://pdbe.org/6bld PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bld RCSB], [http://www.ebi.ac.uk/pdbsum/6bld PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bld ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bld OCA], [http://pdbe.org/6bld PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bld RCSB], [http://www.ebi.ac.uk/pdbsum/6bld PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bld ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Mycmm]]
	[[Category: Bell, S G]]		[[Category: Bell, S G]]
	[[Category: Bruning, J B]]		[[Category: Bruning, J B]]

OCA at 07:12, 31 January 2018

OCA — Wed, 31 Jan 2018 07:12:01 GMT

←Older revision		Revision as of 07:12, 31 January 2018
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bld OCA], [http://pdbe.org/6bld PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bld RCSB], [http://www.ebi.ac.uk/pdbsum/6bld PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bld ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bld OCA], [http://pdbe.org/6bld PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bld RCSB], [http://www.ebi.ac.uk/pdbsum/6bld PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bld ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Members of the cytochrome P450 monooxygenase family CYP268 are found across a broad range of Mycobacterium species including the pathogens Mycobacterium avium, M. colombiense,M. kansasii and Mmarinum CYP268A2, from M. marinum , which is the first member of this family to be studied, was purified and characterised. CYP268A2 was found to bind a variety of substrates with high affinity, including branched and straight-chain fatty acids (C10-C12), acetate esters, and aromatic compounds. The enzyme was also found to bind phenylimidazole inhibitors but not larger azoles, such as ketoconazole. The monooxygenase activity of CYP268A2 was efficiently reconstituted using heterologous electron transfer partner proteins. CYP268A2 hydroxylated geranyl acetate and tran s-pseudoionone at a terminal methyl group to yield ( 2E,6E )-8-hydroxy-3,7-dimethylocta-2,6-dien-1-yl acetate and ( 3E,5E,9E )-11-hydroxy-6,10-dimethylundeca-3,5,9-trien-2-one, respectively. The X-ray crystal structure of CYP268A2 was solved to a resolution of 2.0 A with trans -pseudoionone bound in the active site. The overall structure was similar to that of the related phytanic acid monooxygenase CYP124A1 enzyme from Mycobacterium tuberculosis , which shares 41 % sequence identity. The active site is predominantly hydrophobic but includes the Ser99 and Gln209 residues which form hydrogen bonds with the terminal carbonyl group of the pseudoionone. The structure provided an explanation on why CYP268A2 shows a preference for shorter substrates over the longer chain fatty acids which bind to CYP124A1 and the selective nature of the catalysed monooxygenase activity.
		+
		+	Structural and functional characterisation of the cytochrome P450 enzyme CYP268A2 from Mycobacterium marinum.,Child SA, Naumann EF, Bruning JB, Bell SG Biochem J. 2018 Jan 17. pii: BCJ20170946. doi: 10.1042/BCJ20170946. PMID:29343612<ref>PMID:29343612</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6bld" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 18:29, 24 January 2018

OCA — Wed, 24 Jan 2018 18:29:39 GMT

←Older revision		Revision as of 18:29, 24 January 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6bld is ~~ON HOLD until Paper Publication~~	+	==Mycobacterium marinum cytochrome P450 CYP268A2 in complex with pseudoionone==
-		+	<StructureSection load='6bld' size='340' side='right' caption='[[6bld]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6bld]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BLD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BLD FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DXJ:(3E,5E)-6,10-dimethylundeca-3,5,9-trien-2-one'>DXJ</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bld FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bld OCA], [http://pdbe.org/6bld PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bld RCSB], [http://www.ebi.ac.uk/pdbsum/6bld PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bld ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bell, S G]]
		+	[[Category: Bruning, J B]]
		+	[[Category: Child, S A]]
		+	[[Category: Cytochrome p450]]
		+	[[Category: Heme protein]]
		+	[[Category: Monooxygenase]]
		+	[[Category: Oxidoreductase]]

OCA at 04:58, 20 December 2017

OCA — Wed, 20 Dec 2017 04:58:04 GMT

←Older revision		Revision as of 04:58, 20 December 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6bld is ON HOLD	+	The entry 6bld is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6bld" [edit=sysop:move=sysop]

OCA — Wed, 15 Nov 2017 06:26:54 GMT

Protected "6bld" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:26, 15 November 2017

OCA: New page: '''Unreleased structure''' The entry 6bld is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Nov 2017 06:26:53 GMT

New page: '''Unreleased structure''' The entry 6bld is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6bld is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]