6br0 - Revision history

OCA at 10:38, 14 June 2023

OCA — Wed, 14 Jun 2023 10:38:39 GMT

←Older revision		Revision as of 10:38, 14 June 2023
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	==Solution NMR structure for CcoTx-I==		==Solution NMR structure for CcoTx-I==
-	<StructureSection load='6br0' size='340' side='right'caption='[[6br0~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='6br0' size='340' side='right'caption='[[6br0]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6br0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BR0 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BR0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6br0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ceratogyrus_marshalli Ceratogyrus marshalli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BR0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BR0 FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6br0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6br0 OCA], [~~http~~://pdbe.org/6br0 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6br0 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6br0 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6br0 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6br0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6br0 OCA], [https://pdbe.org/6br0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6br0 RCSB], [https://www.ebi.ac.uk/pdbsum/6br0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6br0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TX1_CERMR TX1_CERMR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ceratogyrus marshalli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Agwa~~, A J~~]]	+	[[Category: Agwa AJ]]
-	[[Category: Schroeder~~, C I]]~~	+	[[Category: Schroeder CI]]
-	~~[[Category: Disulfide]]~~	+
-	~~[[Category: Ick]]~~	+
-	~~[[Category: Nav1 7]]~~	+
-	~~[[Category: Pain]]~~	+
-	~~[[Category: Spider]]~~	+
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Voltage gated ion channel~~]]	+

OCA at 08:36, 8 January 2020

OCA — Wed, 08 Jan 2020 08:36:19 GMT

←Older revision		Revision as of 08:36, 8 January 2020
Line 1:		Line 1:

	==Solution NMR structure for CcoTx-I==		==Solution NMR structure for CcoTx-I==
-	<StructureSection load='6br0' size='340' side='right' caption='[[6br0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='6br0' size='340' side='right'caption='[[6br0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6br0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BR0 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6br0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BR0 FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Agwa, A J]]		[[Category: Agwa, A J]]
	[[Category: Schroeder, C I]]		[[Category: Schroeder, C I]]

OCA at 07:43, 23 May 2018

OCA — Wed, 23 May 2018 07:43:24 GMT

←Older revision		Revision as of 07:43, 23 May 2018
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6br0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6br0 OCA], [http://pdbe.org/6br0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6br0 RCSB], [http://www.ebi.ac.uk/pdbsum/6br0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6br0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6br0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6br0 OCA], [http://pdbe.org/6br0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6br0 RCSB], [http://www.ebi.ac.uk/pdbsum/6br0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6br0 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gating modifier toxins (GMTs) are venom-derived peptides isolated from spiders and other venomous creatures that modulate activity of disease-relevant voltage-gated ion channels and are therefore being pursued as therapeutic leads. The amphipathic surface profile of GMTs has prompted the proposal that some GMTs simultaneously bind to the cell membrane and voltage-gated ion channels in a trimolecular complex. Here we examined whether there is a relationship among spider GMT amphipathicity, membrane binding and potency or selectivity for voltage-gated sodium (NaV) channels. We used NMR spectroscopy and in silico calculations to examine the structures and physicochemical properties of a panel of nine GMTs and deployed surface plasmon resonance to measure GMT affinity for lipids putatively found in proximity to NaV channels. Electrophysiology was used to quantify GMT activity on NaV1.7, an ion channel linked to chronic pain. Selectivity of the peptides was further examined against a panel of NaV channel subtypes. We show that GMTs adsorb to the outer leaflet of anionic lipid bilayers through electrostatic interactions. We did not observe a direct correlation between GMT amphipathicity and affinity for lipid bilayers. Furthermore, GMT-lipid bilayer interactions did not correlate with potency or selectivity for NaVs. We therefore propose that increased membrane binding is unlikely to improve subtype selectivity and that the conserved amphipathic GMT surface profile is an adaptation that facilitates simultaneous modulation of multiple NaVs.
		+
		+	Gating modifier toxins isolated from spider venom: modulation of voltage-gated sodium channels and the role of lipid membranes.,Agwa AJ, Peigneur S, Chow CY, Lawrence N, Craik DJ, Tytgat J, King GF, Henriques ST, Schroeder CI J Biol Chem. 2018 Apr 27. pii: RA118.002553. doi: 10.1074/jbc.RA118.002553. PMID:29703751<ref>PMID:29703751</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6br0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 05:29, 16 May 2018

OCA — Wed, 16 May 2018 05:29:11 GMT

←Older revision		Revision as of 05:29, 16 May 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6br0 ~~is ON HOLD until Paper Publication~~	+	==Solution NMR structure for CcoTx-I==
-		+	<StructureSection load='6br0' size='340' side='right' caption='[[6br0]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~Authors~~: ~~Agwa, A~~.J.~~, Schroeder, C~~.I.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6br0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BR0 FirstGlance]. <br>
-	~~Description~~: ~~Solution NMR structure for CcoTx~~-I	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6br0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6br0 OCA], [http://pdbe.org/6br0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6br0 RCSB], [http://www.ebi.ac.uk/pdbsum/6br0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6br0 ProSAT]</span></td></tr>
-	[[Category: Agwa, A.J]]	+	</table>
-	[[Category: Schroeder, C.I]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Agwa, A J]]
		+	[[Category: Schroeder, C I]]
		+	[[Category: Disulfide]]
		+	[[Category: Ick]]
		+	[[Category: Nav1 7]]
		+	[[Category: Pain]]
		+	[[Category: Spider]]
		+	[[Category: Toxin]]
		+	[[Category: Voltage gated ion channel]]

OCA at 05:15, 15 February 2018

OCA — Thu, 15 Feb 2018 05:15:12 GMT

←Older revision		Revision as of 05:15, 15 February 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6br0 is ON HOLD	+	The entry 6br0 is ON HOLD until Paper Publication

	Authors: Agwa, A.J., Schroeder, C.I.		Authors: Agwa, A.J., Schroeder, C.I.

-	Description: CcoTx-I	+	Description: Solution NMR structure for CcoTx-I
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Agwa, A.J]]		[[Category: Agwa, A.J]]
	[[Category: Schroeder, C.I]]		[[Category: Schroeder, C.I]]

OCA: Protected "6br0" [edit=sysop:move=sysop]

OCA — Wed, 06 Dec 2017 06:07:15 GMT

Protected "6br0" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:07, 6 December 2017

OCA: New page: '''Unreleased structure''' The entry 6br0 is ON HOLD Authors: Agwa, A.J., Schroeder, C.I. Description: CcoTx-I Category: Unreleased Structures Category: Agwa, A.J [[Category: S...

OCA — Wed, 06 Dec 2017 06:07:14 GMT

New page: '''Unreleased structure''' The entry 6br0 is ON HOLD Authors: Agwa, A.J., Schroeder, C.I. Description: CcoTx-I Category: Unreleased Structures Category: Agwa, A.J [[Category: S...

New page

'''Unreleased structure'''

The entry 6br0 is ON HOLD

Authors: Agwa, A.J., Schroeder, C.I.

Description: CcoTx-I
[[Category: Unreleased Structures]]
[[Category: Agwa, A.J]]
[[Category: Schroeder, C.I]]