6bs3 - Revision history

OCA at 14:24, 13 March 2024

OCA — Wed, 13 Mar 2024 14:24:11 GMT

←Older revision		Revision as of 14:24, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6bs3' size='340' side='right'caption='[[6bs3]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='6bs3' size='340' side='right'caption='[[6bs3]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bs3]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BS3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BS3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bs3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BS3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BS3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3679, MTV025.027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), LH57_20055 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bs3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bs3 OCA], [~~http~~://pdbe.org/6bs3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bs3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bs3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bs3 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bs3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bs3 OCA], [https://pdbe.org/6bs3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bs3 RCSB], [https://www.ebi.ac.uk/pdbsum/6bs3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bs3 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Y3679_MYCTU Y3679_MYCTU]
-	We characterized an operon in Mycobacterium tuberculosis, Rv3679-Rv3680, in which each open reading frame is annotated to encode "anion transporter ATPase" homologues. Using structure prediction modeling, we found that Rv3679 and Rv3680 more closely resemble the guided entry of tail-anchored proteins 3 (Get3) chaperone in eukaryotes. Get3 delivers proteins into the membranes of the endoplasmic reticulum and is essential for the normal growth and physiology of some eukaryotes. We sought to characterize the structures of Rv3679 and Rv3680 and test if they have a role in M. tuberculosis pathogenesis. We solved crystal structures of the nucleotide-bound Rv3679-Rv3680 complex at 2.5 to 3.2 A and show that while it has some similarities to Get3 and ArsA, there are notable differences, including that these proteins are unlikely to be involved in anion transport. Deletion of both genes did not reveal any conspicuous growth defects in vitro or in mice. Collectively, we identified a new class of proteins in bacteria with similarity to Get3 complexes, the functions of which remain to be determined.IMPORTANCE Numerous bacterial species encode proteins predicted to have similarity with Get3- and ArsA-type anion transporters. Our studies provide evidence that these proteins, which we named BagA and BagB, are unlikely to be involved in anion transport. In addition, BagA and BagB are conserved in all mycobacterial species, including the causative agent of leprosy, which has a highly decayed genome. This conservation suggests that BagAB constitutes a part of the core mycobacterial genome and is needed for some yet-to-be-determined part of the life cycle of these organisms.	+
-		+
-	Characterization of Guided Entry of Tail-Anchored Proteins 3 Homologues in Mycobacterium tuberculosis.,Hu K, Jordan AT, Zhang S, Dhabaria A, Kovach A, Rangel MV, Ueberheide B, Li H, Darwin KH J Bacteriol. 2019 Jun 21;201(14). pii: ~~JB.00159-19. doi: 10.1128~~/~~JB.00159-19., Print 2019 Jul 15. PMID:31036728<ref>PMID:31036728<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6bs3" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Hu, K]]	+	[[Category: Hu K]]
-	[[Category: Kovach, A]]	+	[[Category: Kovach A]]
-	[[Category: Li, H]]	+	[[Category: Li H]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 06:43, 31 July 2019

OCA — Wed, 31 Jul 2019 06:43:50 GMT

←Older revision		Revision as of 06:43, 31 July 2019
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	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bs3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bs3 OCA], [http://pdbe.org/6bs3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bs3 RCSB], [http://www.ebi.ac.uk/pdbsum/6bs3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bs3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bs3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bs3 OCA], [http://pdbe.org/6bs3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bs3 RCSB], [http://www.ebi.ac.uk/pdbsum/6bs3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bs3 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We characterized an operon in Mycobacterium tuberculosis, Rv3679-Rv3680, in which each open reading frame is annotated to encode "anion transporter ATPase" homologues. Using structure prediction modeling, we found that Rv3679 and Rv3680 more closely resemble the guided entry of tail-anchored proteins 3 (Get3) chaperone in eukaryotes. Get3 delivers proteins into the membranes of the endoplasmic reticulum and is essential for the normal growth and physiology of some eukaryotes. We sought to characterize the structures of Rv3679 and Rv3680 and test if they have a role in M. tuberculosis pathogenesis. We solved crystal structures of the nucleotide-bound Rv3679-Rv3680 complex at 2.5 to 3.2 A and show that while it has some similarities to Get3 and ArsA, there are notable differences, including that these proteins are unlikely to be involved in anion transport. Deletion of both genes did not reveal any conspicuous growth defects in vitro or in mice. Collectively, we identified a new class of proteins in bacteria with similarity to Get3 complexes, the functions of which remain to be determined.IMPORTANCE Numerous bacterial species encode proteins predicted to have similarity with Get3- and ArsA-type anion transporters. Our studies provide evidence that these proteins, which we named BagA and BagB, are unlikely to be involved in anion transport. In addition, BagA and BagB are conserved in all mycobacterial species, including the causative agent of leprosy, which has a highly decayed genome. This conservation suggests that BagAB constitutes a part of the core mycobacterial genome and is needed for some yet-to-be-determined part of the life cycle of these organisms.
		+
		+	Characterization of Guided Entry of Tail-Anchored Proteins 3 Homologues in Mycobacterium tuberculosis.,Hu K, Jordan AT, Zhang S, Dhabaria A, Kovach A, Rangel MV, Ueberheide B, Li H, Darwin KH J Bacteriol. 2019 Jun 21;201(14). pii: JB.00159-19. doi: 10.1128/JB.00159-19., Print 2019 Jul 15. PMID:31036728<ref>PMID:31036728</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6bs3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:21, 21 May 2019

OCA — Tue, 21 May 2019 07:21:11 GMT

←Older revision		Revision as of 07:21, 21 May 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6bs3 is ON HOLD until Jun 05 2020~~	+	==Crystal structure of ADP-bound bacterial Get3-like A and B in Mycobacterium tuberculosis==
-		+	<StructureSection load='6bs3' size='340' side='right'caption='[[6bs3]], [[Resolution\|resolution]] 2.30Å' scene=''>
-	~~Authors: Li, H., Hu, K., Kovach, A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6bs3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BS3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BS3 FirstGlance]. <br>
-	~~Description:~~ Crystal structure of ADP-bound bacterial Get3-like A and B in Mycobacterium tuberculosis	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Rv3679, MTV025.027 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU]), LH57_20055 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
-	[[Category: ~~Li, H~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bs3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bs3 OCA], [http://pdbe.org/6bs3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bs3 RCSB], [http://www.ebi.ac.uk/pdbsum/6bs3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bs3 ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Myctu]]
	[[Category: Hu, K]]		[[Category: Hu, K]]
	[[Category: Kovach, A]]		[[Category: Kovach, A]]
		+	[[Category: Li, H]]
		+	[[Category: Complex]]
		+	[[Category: Unknown function]]

OCA at 03:53, 17 April 2019

OCA — Wed, 17 Apr 2019 03:53:42 GMT

←Older revision		Revision as of 03:53, 17 April 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6bs3 is ON HOLD	+	The entry 6bs3 is ON HOLD until Jun 05 2020

	Authors: Li, H., Hu, K., Kovach, A.		Authors: Li, H., Hu, K., Kovach, A.

OCA at 05:15, 10 April 2019

OCA — Wed, 10 Apr 2019 05:15:55 GMT

←Older revision		Revision as of 05:15, 10 April 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6bs3 is ON HOLD ~~until Jun 05 2020~~	+	The entry 6bs3 is ON HOLD

	Authors: Li, H., Hu, K., Kovach, A.		Authors: Li, H., Hu, K., Kovach, A.

OCA at 06:09, 3 October 2018

OCA — Wed, 03 Oct 2018 06:09:49 GMT

←Older revision		Revision as of 06:09, 3 October 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6bs3 is ON HOLD until ~~Dec 01 2019~~	+	The entry 6bs3 is ON HOLD until Jun 05 2020

	Authors: Li, H., Hu, K., Kovach, A.		Authors: Li, H., Hu, K., Kovach, A.

OCA at 17:13, 24 January 2018

OCA — Wed, 24 Jan 2018 17:13:58 GMT

←Older revision		Revision as of 17:13, 24 January 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6bs3 is ON HOLD	+	The entry 6bs3 is ON HOLD until Dec 01 2019

	Authors: Li, H., Hu, K., Kovach, A.		Authors: Li, H., Hu, K., Kovach, A.

OCA: Protected "6bs3" [edit=sysop:move=sysop]

OCA — Wed, 13 Dec 2017 05:52:43 GMT

Protected "6bs3" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:52, 13 December 2017

OCA: New page: '''Unreleased structure''' The entry 6bs3 is ON HOLD Authors: Li, H., Hu, K., Kovach, A. Description: Crystal structure of ADP-bound bacterial Get3-like A and B in Mycobacterium tuberc...

OCA — Wed, 13 Dec 2017 05:52:41 GMT

New page: '''Unreleased structure''' The entry 6bs3 is ON HOLD Authors: Li, H., Hu, K., Kovach, A. Description: Crystal structure of ADP-bound bacterial Get3-like A and B in Mycobacterium tuberc...

New page

'''Unreleased structure'''

The entry 6bs3 is ON HOLD

Authors: Li, H., Hu, K., Kovach, A.

Description: Crystal structure of ADP-bound bacterial Get3-like A and B in Mycobacterium tuberculosis
[[Category: Unreleased Structures]]
[[Category: Li, H]]
[[Category: Hu, K]]
[[Category: Kovach, A]]