6bvg - Revision history

OCA at 14:51, 4 October 2023

OCA — Wed, 04 Oct 2023 14:51:07 GMT

←Older revision		Revision as of 14:51, 4 October 2023
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	<StructureSection load='6bvg' size='340' side='right'caption='[[6bvg]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='6bvg' size='340' side='right'caption='[[6bvg]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bvg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baccz Baccz~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BVG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BVG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bvg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_E33L Bacillus cereus E33L]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BVG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BVG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=HG:MERCURY+(II)+ION'>HG</scene~~>, ~~<scene name='pdbligand=MAL:MALTOSE'>MAL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ptsG, BCE33L0344 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=288681 BACCZ])</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bvg OCA], [https://pdbe.org/6bvg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bvg RCSB], [https://www.ebi.ac.uk/pdbsum/6bvg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bvg ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bvg OCA], [~~http~~://pdbe.org/6bvg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bvg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bvg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bvg ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/Q63GK8_BACCZ Q63GK8_BACCZ]] The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.[SAAS:SAAS00547063]	+	[https://www.uniprot.org/uniprot/Q63GK8_BACCZ Q63GK8_BACCZ] The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.[SAAS:SAAS00547063]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Baccz~~]]	+	[[Category: Bacillus cereus E33L]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ren, Z]]	+	[[Category: Ren Z]]
-	[[Category: Zhou, M]]	+	[[Category: Zhou M]]
-	~~[[Category: Eiic maltose transporter]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 10:28, 1 January 2020

OCA — Wed, 01 Jan 2020 10:28:01 GMT

←Older revision		Revision as of 10:28, 1 January 2020
Line 1:		Line 1:

	==Crystal structure of bcMalT T280C-E54C crosslinked by divalent mercury==		==Crystal structure of bcMalT T280C-E54C crosslinked by divalent mercury==
-	<StructureSection load='6bvg' size='340' side='right' caption='[[6bvg]], [[Resolution\|resolution]] 3.20Å' scene=''>	+	<StructureSection load='6bvg' size='340' side='right'caption='[[6bvg]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bvg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baccz Baccz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BVG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BVG FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bvg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baccz Baccz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BVG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BVG FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Baccz]]		[[Category: Baccz]]
		+	[[Category: Large Structures]]
	[[Category: Ren, Z]]		[[Category: Ren, Z]]
	[[Category: Zhou, M]]		[[Category: Zhou, M]]
	[[Category: Eiic maltose transporter]]		[[Category: Eiic maltose transporter]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 06:19, 6 June 2018

OCA — Wed, 06 Jun 2018 06:19:22 GMT

←Older revision		Revision as of 06:19, 6 June 2018
Line 3:		Line 3:
	<StructureSection load='6bvg' size='340' side='right' caption='[[6bvg]], [[Resolution\|resolution]] 3.20Å' scene=''>		<StructureSection load='6bvg' size='340' side='right' caption='[[6bvg]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bvg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BVG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BVG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bvg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Baccz Baccz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BVG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BVG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ptsG, BCE33L0344 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=288681 BACCZ])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bvg OCA], [http://pdbe.org/6bvg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bvg RCSB], [http://www.ebi.ac.uk/pdbsum/6bvg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bvg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bvg OCA], [http://pdbe.org/6bvg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bvg RCSB], [http://www.ebi.ac.uk/pdbsum/6bvg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bvg ProSAT]</span></td></tr>
Line 10:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/Q63GK8_BACCZ Q63GK8_BACCZ]] The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.[SAAS:SAAS00547063]		[[http://www.uniprot.org/uniprot/Q63GK8_BACCZ Q63GK8_BACCZ]] The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.[SAAS:SAAS00547063]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The phosphoenolpyruvate-dependent phosphotransferase system (PTS) transports sugar into bacteria and phosphorylates the sugar for metabolic consumption. The PTS is important for the survival of bacteria and thus a potential target for antibiotics, but its mechanism of sugar uptake and phosphorylation remains unclear. The PTS is composed of multiple proteins, and the membrane-embedded Enzyme IIC (EIIC) component transports sugars across the membrane. Crystal structures of two members of the glucose superfamily of EIICs, bcChbC and bcMalT, were solved in the inward-facing and outward-facing conformations, and the structures suggest that sugar translocation could be achieved by movement of a structured domain that contains the sugar-binding site. However, different conformations have not been captured on the same transporter to allow precise description of the conformational changes. Here we present a crystal structure of bcMalT trapped in an inward-facing conformation by a mercury ion that bridges two strategically placed cysteine residues. The structure allows direct comparison of the outward- and inward-facing conformations and reveals a large rigid-body motion of the sugar-binding domain and other conformational changes that accompany the rigid-body motion. All-atom molecular dynamics simulations show that the inward-facing structure is stable with or without the cross-linking. The conformational changes were further validated by single-molecule Foster resonance energy transfer (smFRET). Combined, these results establish the elevator-type mechanism of transport in the glucose superfamily of EIIC transporters.
		+
		+	Structure of an EIIC sugar transporter trapped in an inward-facing conformation.,Ren Z, Lee J, Moosa MM, Nian Y, Hu L, Xu Z, McCoy JG, Ferreon ACM, Im W, Zhou M Proc Natl Acad Sci U S A. 2018 May 21. pii: 1800647115. doi:, 10.1073/pnas.1800647115. PMID:29784777<ref>PMID:29784777</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6bvg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baccz]]
	[[Category: Ren, Z]]		[[Category: Ren, Z]]
	[[Category: Zhou, M]]		[[Category: Zhou, M]]
	[[Category: Eiic maltose transporter]]		[[Category: Eiic maltose transporter]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 07:21, 23 May 2018

OCA — Wed, 23 May 2018 07:21:19 GMT

←Older revision		Revision as of 07:21, 23 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6bvg is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of bcMalT T280C-E54C crosslinked by divalent mercury==
-		+	<StructureSection load='6bvg' size='340' side='right' caption='[[6bvg]], [[Resolution\|resolution]] 3.20Å' scene=''>
-	~~Authors~~: ~~Ren~~, Z., ~~Zhou~~, M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6bvg]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BVG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BVG FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of bcMalT T280C~~-~~E54C crosslinked by divalent mercury~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bvg OCA], [http://pdbe.org/6bvg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bvg RCSB], [http://www.ebi.ac.uk/pdbsum/6bvg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bvg ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q63GK8_BACCZ Q63GK8_BACCZ]] The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane.[SAAS:SAAS00547063]
		+	__TOC__
		+	</StructureSection>
	[[Category: Ren, Z]]		[[Category: Ren, Z]]
	[[Category: Zhou, M]]		[[Category: Zhou, M]]
		+	[[Category: Eiic maltose transporter]]
		+	[[Category: Transport protein]]

OCA at 06:36, 2 May 2018

OCA — Wed, 02 May 2018 06:36:52 GMT

←Older revision		Revision as of 06:36, 2 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6bvg is ON HOLD	+	The entry 6bvg is ON HOLD until Paper Publication

	Authors: Ren, Z., Zhou, M.		Authors: Ren, Z., Zhou, M.

OCA at 12:23, 11 April 2018

OCA — Wed, 11 Apr 2018 12:23:25 GMT

←Older revision		Revision as of 12:23, 11 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6bvg is ON HOLD ~~until Paper Publication~~	+	The entry 6bvg is ON HOLD

	Authors: Ren, Z., Zhou, M.		Authors: Ren, Z., Zhou, M.

OCA at 07:10, 10 January 2018

OCA — Wed, 10 Jan 2018 07:10:53 GMT

←Older revision		Revision as of 07:10, 10 January 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6bvg is ON HOLD	+	The entry 6bvg is ON HOLD until Paper Publication

	Authors: Ren, Z., Zhou, M.		Authors: Ren, Z., Zhou, M.

OCA at 07:18, 27 December 2017

OCA — Wed, 27 Dec 2017 07:18:35 GMT

←Older revision		Revision as of 07:18, 27 December 2017
Line 5:		Line 5:
	Authors: Ren, Z., Zhou, M.		Authors: Ren, Z., Zhou, M.

-	Description: ~~crystal~~ structure of bcMalT T280C-E54C crosslinked by divalent mercury	+	Description: Crystal structure of bcMalT T280C-E54C crosslinked by divalent mercury
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Ren, Z]]		[[Category: Ren, Z]]
	[[Category: Zhou, M]]		[[Category: Zhou, M]]

OCA: Protected "6bvg" [edit=sysop:move=sysop]

OCA — Wed, 20 Dec 2017 05:03:43 GMT

Protected "6bvg" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:03, 20 December 2017

OCA: New page: '''Unreleased structure''' The entry 6bvg is ON HOLD Authors: Ren, Z., Zhou, M. Description: crystal structure of bcMalT T280C-E54C crosslinked by divalent mercury [[Category: Unreleas...

OCA — Wed, 20 Dec 2017 05:03:42 GMT

New page: '''Unreleased structure''' The entry 6bvg is ON HOLD Authors: Ren, Z., Zhou, M. Description: crystal structure of bcMalT T280C-E54C crosslinked by divalent mercury [[Category: Unreleas...

New page

'''Unreleased structure'''

The entry 6bvg is ON HOLD

Authors: Ren, Z., Zhou, M.

Description: crystal structure of bcMalT T280C-E54C crosslinked by divalent mercury
[[Category: Unreleased Structures]]
[[Category: Ren, Z]]
[[Category: Zhou, M]]