6bx4 - Revision history

OCA at 14:51, 4 October 2023

OCA — Wed, 04 Oct 2023 14:51:55 GMT

←Older revision		Revision as of 14:51, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='6bx4' size='340' side='right'caption='[[6bx4]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='6bx4' size='340' side='right'caption='[[6bx4]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bx4]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BX4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BX4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bx4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BX4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=F:FLUORIDE+ION'>F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">crcB, A4T40_27000, A8M81_25235, AC789_145pl00540, AKG99_27195, B7C53_24430, BET08_05210, BIU72_24510, BK292_28205, BK334_22235, BK373_23795, BMR46_27175, BMR58_00505, BMR59_25090, BMR61_25795, CDL37_21115, CNQ53_00195, ECONIH1_26550, ECS286_0026, MJ49_27125, pCTXM15_EC8_00123, pO103_22 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=F:FLUORIDE+ION'>F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bx4 OCA], [~~http~~://pdbe.org/6bx4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bx4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bx4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bx4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bx4 OCA], [https://pdbe.org/6bx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bx4 RCSB], [https://www.ebi.ac.uk/pdbsum/6bx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bx4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/Q6J5N4_ECOLX Q6J5N4_ECOLX]] Important for reducing fluoride concentration in the cell, thus reducing its toxicity.[HAMAP-Rule:MF_00454][SAAS:SAAS00096000]	+	[https://www.uniprot.org/uniprot/Q6J5N4_ECOLX Q6J5N4_ECOLX] Important for reducing fluoride concentration in the cell, thus reducing its toxicity.[HAMAP-Rule:MF_00454][SAAS:SAAS00096000]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Miller, C]]	+	[[Category: Miller C]]
-	[[Category: Turman~~, D L]]~~	+	[[Category: Turman DL]]
-	~~[[Category: Fluc]]~~	+
-	~~[[Category: Fluoride channel]]~~	+
-	~~[[Category: Monobody]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 10:29, 1 January 2020

OCA — Wed, 01 Jan 2020 10:29:38 GMT

←Older revision		Revision as of 10:29, 1 January 2020
Line 1:		Line 1:

	==The crystal structure of fluoride channel Fluc Ec2 with Monobody S9==		==The crystal structure of fluoride channel Fluc Ec2 with Monobody S9==
-	<StructureSection load='6bx4' size='340' side='right' caption='[[6bx4]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='6bx4' size='340' side='right'caption='[[6bx4]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bx4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BX4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bx4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BX4 FirstGlance]. <br>
Line 25:		Line 25:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Miller, C]]		[[Category: Miller, C]]
	[[Category: Turman, D L]]		[[Category: Turman, D L]]

OCA at 07:17, 28 February 2018

OCA — Wed, 28 Feb 2018 07:17:18 GMT

←Older revision		Revision as of 07:17, 28 February 2018
Line 3:		Line 3:
	<StructureSection load='6bx4' size='340' side='right' caption='[[6bx4]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='6bx4' size='340' side='right' caption='[[6bx4]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bx4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BX4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bx4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BX4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=F:FLUORIDE+ION'>F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=F:FLUORIDE+ION'>F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">crcB, A4T40_27000, A8M81_25235, AC789_145pl00540, AKG99_27195, B7C53_24430, BET08_05210, BIU72_24510, BK292_28205, BK334_22235, BK373_23795, BMR46_27175, BMR58_00505, BMR59_25090, BMR61_25795, CDL37_21115, CNQ53_00195, ECONIH1_26550, ECS286_0026, MJ49_27125, pCTXM15_EC8_00123, pO103_22 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bx4 OCA], [http://pdbe.org/6bx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6bx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bx4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bx4 OCA], [http://pdbe.org/6bx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6bx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bx4 ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
		+	[[Category: Human]]
	[[Category: Miller, C]]		[[Category: Miller, C]]
	[[Category: Turman, D L]]		[[Category: Turman, D L]]

OCA at 06:34, 15 February 2018

OCA — Thu, 15 Feb 2018 06:34:32 GMT

←Older revision		Revision as of 06:34, 15 February 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6bx4 is ~~ON HOLD~~	+	==The crystal structure of fluoride channel Fluc Ec2 with Monobody S9==
		+	<StructureSection load='6bx4' size='340' side='right' caption='[[6bx4]], [[Resolution\|resolution]] 2.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6bx4]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BX4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BX4 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DMU:DECYL-BETA-D-MALTOPYRANOSIDE'>DMU</scene>, <scene name='pdbligand=F:FLUORIDE+ION'>F</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bx4 OCA], [http://pdbe.org/6bx4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bx4 RCSB], [http://www.ebi.ac.uk/pdbsum/6bx4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bx4 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q6J5N4_ECOLX Q6J5N4_ECOLX]] Important for reducing fluoride concentration in the cell, thus reducing its toxicity.[HAMAP-Rule:MF_00454][SAAS:SAAS00096000]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Fluoride ion channels of the Fluc family selectively export F(-) ions to rescue unicellular organisms from acute F(-) toxicity. Crystal structures of bacterial Fluc channels in complex with synthetic monobodies, fibronectin-derived soluble beta-sandwich fold proteins, show 2-fold symmetric homodimers with an antiparallel transmembrane topology. Monobodies also block Fluc F(-) current via a pore blocking mechanism. However, little is known about the energetic contributions of individual monobody residues to the affinity of the monobody-channel complex or whether the structural paratope corresponds to functional reality. This study seeks to structurally identify and compare residues interacting with Fluc between two highly similar monobodies and subjects them to mutagenesis and functional measurements of equilibrium affinities via a fluorescence anisotropy binding assay to determine their energetic contributions. The results indicate that the functional and structural paratopes strongly agree and that many Tyr residues at the interface, while playing a key role in affinity, can be substituted with Phe and Trp without large disruptions.

-	~~Authors~~:	+	Molecular Interactions between a Fluoride Ion Channel and Synthetic Protein Blockers.,Turman DL, Cheloff AZ, Corrado AD, Nathanson JT, Miller C Biochemistry. 2018 Feb 2. doi: 10.1021/acs.biochem.7b01272. PMID:29393634<ref>PMID:29393634</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6bx4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Miller, C]]
		+	[[Category: Turman, D L]]
		+	[[Category: Fluc]]
		+	[[Category: Fluoride channel]]
		+	[[Category: Monobody]]
		+	[[Category: Transport protein]]

OCA: Protected "6bx4" [edit=sysop:move=sysop]

OCA — Wed, 27 Dec 2017 07:20:10 GMT

Protected "6bx4" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:20, 27 December 2017

OCA: New page: '''Unreleased structure''' The entry 6bx4 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Dec 2017 07:20:09 GMT

New page: '''Unreleased structure''' The entry 6bx4 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6bx4 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]