6bzl - Revision history

OCA at 07:30, 1 May 2024

OCA — Wed, 01 May 2024 07:30:12 GMT

←Older revision		Revision as of 07:30, 1 May 2024
Line 1:		Line 1:

	==Solution structure of VEK75==		==Solution structure of VEK75==
-	<StructureSection load='6bzl' size='340' side='right'caption='[[6bzl~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='6bzl' size='340' side='right'caption='[[6bzl]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bzl]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BZL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BZL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bzl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BZL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bzl OCA], [~~http~~://pdbe.org/6bzl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bzl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bzl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bzl ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bzl OCA], [https://pdbe.org/6bzl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bzl RCSB], [https://www.ebi.ac.uk/pdbsum/6bzl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bzl ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q6V4L8_STRPY Q6V4L8_STRPY]
-	Group A Streptococcus pyogenes (GAS) is a causative agent of pharyngeal and dermal infections in humans. A major virulence determinant of GAS is its dimeric signature fibrillar M-protein (M-Prt), which is evolutionarily designed in modules, ranging from a hypervariable extracellular N-terminal region to a progressively more highly conserved C-terminus that is covalently anchored to the cell wall. Of the >250 GAS isolates classified, only the subset of skin-trophic Pattern D strains expresses a specific serotype of M-Prt, PAM, that directly binds to host human plasminogen (hPg) via its extracellular NH2-terminal variable A-domain region. This interaction allows these GAS strains to accumulate components of the host fibrinolytic system on their surfaces to serve extracellular functions. While structure-function studies have been accomplished on M-Prts from Pattern A-C GAS isolates with different direct ligand binding properties compared to PAM, much less is known regarding the structure-function relationships of PAM-type M-Prts, particularly their dimerization determinants. To examine these questions, PAMs from seven GAS strains with sequence variations in the NH2-terminal ligand binding domains, as well as truncated versions of PAM, were designed and studied. The results from bioinformatic and biophysical analyses show that the different domains of PAM are disparately engaged in dimerization. From these data, we propose an experimentally-based model for PAM secondary and quaternary structures that is highly dependent on the conserved helical C-terminal C-D-domains. In addition, while the N-terminal regions of PAMs are variable in sequence, the binding properties of hPg and its activated product, plasmin, to the A-domain, remain intact.	+
-		+
-	Contributions of different modules of the plasminogen-binding Streptococcus pyogenes M-protein that mediate its functional dimerization.,Qiu C, Yuan Y, Zajicek J, Liang Z, Balsara RD, Brito-Robionson T, Lee SW, Ploplis VA, Castellino FJ J Struct Biol. 2018 Nov;204(2):~~151-164. doi: 10.1016~~/~~j.jsb.2018.07.017. Epub 2018, Jul 30. PMID:30071314<ref>PMID:30071314<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6bzl" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Micrococcus scarlatinae klein 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Castellino, F J~~]]	+	[[Category: Streptococcus pyogenes]]
-	[[Category: ~~Qiu, C~~]]	+	[[Category: Castellino FJ]]
-	[[Category: ~~Yuan, Y~~]]	+	[[Category: Qiu C]]
-	[[Category: ~~Plasminogen binding peptide]]~~	+	[[Category: Yuan Y]]
-	~~[[Category: Protein binding~~]]	+

OCA at 06:44, 31 July 2019

OCA — Wed, 31 Jul 2019 06:44:05 GMT

←Older revision		Revision as of 06:44, 31 July 2019
Line 1:		Line 1:

	==Solution structure of VEK75==		==Solution structure of VEK75==
-	<StructureSection load='6bzl' size='340' side='right' caption='[[6bzl]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>	+	<StructureSection load='6bzl' size='340' side='right'caption='[[6bzl]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bzl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BZL FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bzl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BZL FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bzl OCA], [http://pdbe.org/6bzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bzl RCSB], [http://www.ebi.ac.uk/pdbsum/6bzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bzl ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bzl OCA], [http://pdbe.org/6bzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bzl RCSB], [http://www.ebi.ac.uk/pdbsum/6bzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bzl ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Group A Streptococcus pyogenes (GAS) is a causative agent of pharyngeal and dermal infections in humans. A major virulence determinant of GAS is its dimeric signature fibrillar M-protein (M-Prt), which is evolutionarily designed in modules, ranging from a hypervariable extracellular N-terminal region to a progressively more highly conserved C-terminus that is covalently anchored to the cell wall. Of the >250 GAS isolates classified, only the subset of skin-trophic Pattern D strains expresses a specific serotype of M-Prt, PAM, that directly binds to host human plasminogen (hPg) via its extracellular NH2-terminal variable A-domain region. This interaction allows these GAS strains to accumulate components of the host fibrinolytic system on their surfaces to serve extracellular functions. While structure-function studies have been accomplished on M-Prts from Pattern A-C GAS isolates with different direct ligand binding properties compared to PAM, much less is known regarding the structure-function relationships of PAM-type M-Prts, particularly their dimerization determinants. To examine these questions, PAMs from seven GAS strains with sequence variations in the NH2-terminal ligand binding domains, as well as truncated versions of PAM, were designed and studied. The results from bioinformatic and biophysical analyses show that the different domains of PAM are disparately engaged in dimerization. From these data, we propose an experimentally-based model for PAM secondary and quaternary structures that is highly dependent on the conserved helical C-terminal C-D-domains. In addition, while the N-terminal regions of PAMs are variable in sequence, the binding properties of hPg and its activated product, plasmin, to the A-domain, remain intact.
		+
		+	Contributions of different modules of the plasminogen-binding Streptococcus pyogenes M-protein that mediate its functional dimerization.,Qiu C, Yuan Y, Zajicek J, Liang Z, Balsara RD, Brito-Robionson T, Lee SW, Ploplis VA, Castellino FJ J Struct Biol. 2018 Nov;204(2):151-164. doi: 10.1016/j.jsb.2018.07.017. Epub 2018, Jul 30. PMID:30071314<ref>PMID:30071314</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6bzl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Micrococcus scarlatinae klein 1884]]		[[Category: Micrococcus scarlatinae klein 1884]]
		+	[[Category: Large Structures]]
	[[Category: Castellino, F J]]		[[Category: Castellino, F J]]
	[[Category: Qiu, C]]		[[Category: Qiu, C]]

OCA at 08:08, 21 February 2019

OCA — Thu, 21 Feb 2019 08:08:25 GMT

←Older revision		Revision as of 08:08, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='6bzl' size='340' side='right' caption='[[6bzl]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='6bzl' size='340' side='right' caption='[[6bzl]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6bzl]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BZL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6bzl]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BZL FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bzl OCA], [http://pdbe.org/6bzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bzl RCSB], [http://www.ebi.ac.uk/pdbsum/6bzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bzl ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bzl OCA], [http://pdbe.org/6bzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bzl RCSB], [http://www.ebi.ac.uk/pdbsum/6bzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bzl ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Micrococcus scarlatinae klein 1884]]
	[[Category: Castellino, F J]]		[[Category: Castellino, F J]]
	[[Category: Qiu, C]]		[[Category: Qiu, C]]

OCA at 12:02, 16 January 2019

OCA — Wed, 16 Jan 2019 12:02:23 GMT

←Older revision		Revision as of 12:02, 16 January 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6bzl ~~is ON HOLD until Paper Publication~~	+	==Solution structure of VEK75==
-		+	<StructureSection load='6bzl' size='340' side='right' caption='[[6bzl]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
-	~~Authors~~: ~~Qiu~~, C., ~~Yuan~~, Y., ~~Castellino~~, F.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6bzl]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BZL FirstGlance]. <br>
-	~~Description: Solution structure of VEK75~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bzl OCA], [http://pdbe.org/6bzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bzl RCSB], [http://www.ebi.ac.uk/pdbsum/6bzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bzl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Castellino, F J]]
	[[Category: Qiu, C]]		[[Category: Qiu, C]]
	[[Category: Yuan, Y]]		[[Category: Yuan, Y]]
-	[[Category: ~~Castellino, F.J~~]]	+	[[Category: Plasminogen binding peptide]]
		+	[[Category: Protein binding]]

OCA at 05:16, 15 February 2018

OCA — Thu, 15 Feb 2018 05:16:00 GMT

←Older revision		Revision as of 05:16, 15 February 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6bzl is ON HOLD	+	The entry 6bzl is ON HOLD until Paper Publication

	Authors: Qiu, C., Yuan, Y., Castellino, F.J.		Authors: Qiu, C., Yuan, Y., Castellino, F.J.
Line 7:		Line 7:
	Description: Solution structure of VEK75		Description: Solution structure of VEK75
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Castellino, F.J]]
	[[Category: Qiu, C]]		[[Category: Qiu, C]]
	[[Category: Yuan, Y]]		[[Category: Yuan, Y]]
		+	[[Category: Castellino, F.J]]

OCA: Protected "6bzl" [edit=sysop:move=sysop]

OCA — Wed, 03 Jan 2018 04:55:29 GMT

Protected "6bzl" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:55, 3 January 2018

OCA: New page: '''Unreleased structure''' The entry 6bzl is ON HOLD Authors: Qiu, C., Yuan, Y., Castellino, F.J. Description: Solution structure of VEK75 Category: Unreleased Structures [[Categor...

OCA — Wed, 03 Jan 2018 04:55:28 GMT

New page: '''Unreleased structure''' The entry 6bzl is ON HOLD Authors: Qiu, C., Yuan, Y., Castellino, F.J. Description: Solution structure of VEK75 Category: Unreleased Structures [[Categor...

New page

'''Unreleased structure'''

The entry 6bzl is ON HOLD

Authors: Qiu, C., Yuan, Y., Castellino, F.J.

Description: Solution structure of VEK75
[[Category: Unreleased Structures]]
[[Category: Castellino, F.J]]
[[Category: Qiu, C]]
[[Category: Yuan, Y]]