6c3r - Revision history

OCA at 09:05, 1 May 2019

OCA — Wed, 01 May 2019 09:05:57 GMT

←Older revision		Revision as of 09:05, 1 May 2019
Line 1:		Line 1:

	==Cricket paralysis virus RNAi suppressor protein CrPV-1A==		==Cricket paralysis virus RNAi suppressor protein CrPV-1A==
-	<StructureSection load='6c3r' size='340' side='right' caption='[[6c3r]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='6c3r' size='340' side='right'caption='[[6c3r]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6c3r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crpvc Crpvc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C3R FirstGlance]. <br>		<table><tr><td colspan='2'>[[6c3r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crpvc Crpvc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C3R FirstGlance]. <br>
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/POLN_CRPVC POLN_CRPVC]] Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Inhibits siRNA function, but does not interfere with miRNA pathway. Does not bind to dsRNA or siRNA.<ref>PMID:20400949</ref> RNA-directed RNA polymerase replicates genomic and antigenomic RNA.[PROSITE-ProRule:PRU00539]<ref>PMID:20400949</ref>		[[http://www.uniprot.org/uniprot/POLN_CRPVC POLN_CRPVC]] Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Inhibits siRNA function, but does not interfere with miRNA pathway. Does not bind to dsRNA or siRNA.<ref>PMID:20400949</ref> RNA-directed RNA polymerase replicates genomic and antigenomic RNA.[PROSITE-ProRule:PRU00539]<ref>PMID:20400949</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The dicistrovirus, Cricket paralysis virus (CrPV) encodes an RNA interference (RNAi) suppressor, 1A, which modulates viral virulence. Using the Drosophila model, we combined structural, biochemical, and virological approaches to elucidate the strategies by which CrPV-1A restricts RNAi immunity. The atomic resolution structure of CrPV-1A uncovered a flexible loop that interacts with Argonaute 2 (Ago-2), thereby inhibiting Ago-2 endonuclease-dependent immunity. Mutations disrupting Ago-2 binding attenuates viral pathogenesis in wild-type but not Ago-2-deficient flies. CrPV-1A also contains a BC-box motif that enables the virus to hijack a host Cul2-Rbx1-EloBC ubiquitin ligase complex, which promotes Ago-2 degradation and virus replication. Our study uncovers a viral-based dual regulatory program that restricts antiviral immunity by direct interaction with and modulation of host proteins. While the direct inhibition of Ago-2 activity provides an efficient mechanism to establish infection, the recruitment of a ubiquitin ligase complex enables CrPV-1A to amplify Ago-2 inactivation to restrict further antiviral RNAi immunity.
		+
		+	A Viral Protein Restricts Drosophila RNAi Immunity by Regulating Argonaute Activity and Stability.,Nayak A, Kim DY, Trnka MJ, Kerr CH, Lidsky PV, Stanley DJ, Rivera BM, Li KH, Burlingame AL, Jan E, Frydman J, Gross JD, Andino R Cell Host Microbe. 2018 Oct 10;24(4):542-557.e9. doi: 10.1016/j.chom.2018.09.006. PMID:30308158<ref>PMID:30308158</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6c3r" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
Line 14:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Crpvc]]		[[Category: Crpvc]]
		+	[[Category: Large Structures]]
	[[Category: Andino, R]]		[[Category: Andino, R]]
	[[Category: Gross, J]]		[[Category: Gross, J]]

OCA at 08:12, 21 February 2019

OCA — Thu, 21 Feb 2019 08:12:07 GMT

←Older revision		Revision as of 08:12, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='6c3r' size='340' side='right' caption='[[6c3r]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='6c3r' size='340' side='right' caption='[[6c3r]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6c3r]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C3R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6c3r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Crpvc Crpvc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C3R FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c3r OCA], [http://pdbe.org/6c3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c3r RCSB], [http://www.ebi.ac.uk/pdbsum/6c3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c3r ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ORF1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=928300 CRPVC])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c3r OCA], [http://pdbe.org/6c3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c3r RCSB], [http://www.ebi.ac.uk/pdbsum/6c3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c3r ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Crpvc]]
	[[Category: Andino, R]]		[[Category: Andino, R]]
	[[Category: Gross, J]]		[[Category: Gross, J]]

OCA at 07:55, 17 October 2018

OCA — Wed, 17 Oct 2018 07:55:28 GMT

←Older revision		Revision as of 07:55, 17 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6c3r is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Cricket paralysis virus RNAi suppressor protein CrPV-1A==
-		+	<StructureSection load='6c3r' size='340' side='right' caption='[[6c3r]], [[Resolution\|resolution]] 2.60Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6c3r]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C3R FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c3r OCA], [http://pdbe.org/6c3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c3r RCSB], [http://www.ebi.ac.uk/pdbsum/6c3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c3r ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/POLN_CRPVC POLN_CRPVC]] Protein 1A functions as a suppressor of RNA-mediated gene silencing, an antiviral defense mechanism of insect cells. Inhibits siRNA function, but does not interfere with miRNA pathway. Does not bind to dsRNA or siRNA.<ref>PMID:20400949</ref> RNA-directed RNA polymerase replicates genomic and antigenomic RNA.[PROSITE-ProRule:PRU00539]<ref>PMID:20400949</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Andino, R]]
		+	[[Category: Gross, J]]
		+	[[Category: Kim, D Y]]
		+	[[Category: Nayak, A]]
		+	[[Category: Rnai inhibitor]]
		+	[[Category: Viral protein]]

OCA at 05:07, 7 February 2018

OCA — Wed, 07 Feb 2018 05:07:58 GMT

←Older revision		Revision as of 05:07, 7 February 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6c3r is ON HOLD	+	The entry 6c3r is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6c3r" [edit=sysop:move=sysop]

OCA — Wed, 17 Jan 2018 05:46:31 GMT

Protected "6c3r" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:46, 17 January 2018

OCA: New page: '''Unreleased structure''' The entry 6c3r is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 17 Jan 2018 05:46:30 GMT

New page: '''Unreleased structure''' The entry 6c3r is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6c3r is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]