6c7s - Revision history

OCA at 14:57, 4 October 2023

OCA — Wed, 04 Oct 2023 14:57:52 GMT

←Older revision		Revision as of 14:57, 4 October 2023
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	<StructureSection load='6c7s' size='340' side='right'caption='[[6c7s]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6c7s' size='340' side='right'caption='[[6c7s]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6c7s]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Nocfa Nocfa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C7S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6C7S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6c7s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nocardia_farcinica_IFM_10152 Nocardia farcinica IFM 10152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C7S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6C7S FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=RFH:(1E,3S,4R,5S,6R,7R,8R,9S,10S,11E,13E)-15-amino-1-{[(2S)-5,7-dihydroxy-2,4-dimethyl-8-{(E)-[(4-methylpiperazin-1-yl)imino]methyl}-1,6,9-trioxo-1,2,6,9-tetrahydronaphtho[2,1-b]furan-2-yl]oxy}-7,9-dihydroxy-3-methoxy-4,6,8,10,14-pentamethyl-15-oxopentadeca-1,11,13-trien-5-yl+acetate'>RFH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kow\|5kow]], [[5kox\|5kox]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=RFH:(1E,3S,4R,5S,6R,7R,8R,9S,10S,11E,13E)-15-amino-1-{[(2S)-5,7-dihydroxy-2,4-dimethyl-8-{(E)-[(4-methylpiperazin-1-yl)imino]methyl}-1,6,9-trioxo-1,2,6,9-tetrahydronaphtho[2,1-b]furan-2-yl]oxy}-7,9-dihydroxy-3-methoxy-4,6,8,10,14-pentamethyl-15-oxopentadeca-1,11,13-trien-5-yl+acetate'>RFH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rox, NFA_35380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=247156 NOCFA])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6c7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c7s OCA], [https://pdbe.org/6c7s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6c7s RCSB], [https://www.ebi.ac.uk/pdbsum/6c7s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6c7s ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6c7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c7s OCA], [~~http~~://pdbe.org/6c7s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6c7s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6c7s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6c7s ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ROX_NOCFA ROX_NOCFA] Monooxygenase that can modify rifampicin, thereby inactivating its antibiotic activity (PubMed:19942945, PubMed:29578336). It constitutes a secondary rifampicin resistance factor (PubMed:19942945).<ref>PMID:19942945</ref> <ref>PMID:29578336</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Nocfa~~]]	+	[[Category: Nocardia farcinica IFM 10152]]
-	[[Category: Liu, L K]]	+	[[Category: Liu L-K]]
-	[[Category: Tanner~~, J J]]~~	+	[[Category: Tanner JJ]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Rifampicin~~]]	+

OCA at 08:23, 27 November 2019

OCA — Wed, 27 Nov 2019 08:23:22 GMT

←Older revision		Revision as of 08:23, 27 November 2019
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	==Structure of Rifampicin Monooxygenase with Product Bound==		==Structure of Rifampicin Monooxygenase with Product Bound==
-	<StructureSection load='6c7s' size='340' side='right' caption='[[6c7s]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='6c7s' size='340' side='right'caption='[[6c7s]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6c7s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nocfa Nocfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C7S FirstGlance]. <br>		<table><tr><td colspan='2'>[[6c7s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nocfa Nocfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C7S FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Nocfa]]		[[Category: Nocfa]]
	[[Category: Liu, L K]]		[[Category: Liu, L K]]

OCA at 08:14, 21 February 2019

OCA — Thu, 21 Feb 2019 08:14:28 GMT

←Older revision		Revision as of 08:14, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='6c7s' size='340' side='right' caption='[[6c7s]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6c7s' size='340' side='right' caption='[[6c7s]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6c7s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C7S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6c7s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nocfa Nocfa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C7S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=RFH:(1E,3S,4R,5S,6R,7R,8R,9S,10S,11E,13E)-15-amino-1-{[(2S)-5,7-dihydroxy-2,4-dimethyl-8-{(E)-[(4-methylpiperazin-1-yl)imino]methyl}-1,6,9-trioxo-1,2,6,9-tetrahydronaphtho[2,1-b]furan-2-yl]oxy}-7,9-dihydroxy-3-methoxy-4,6,8,10,14-pentamethyl-15-oxopentadeca-1,11,13-trien-5-yl+acetate'>RFH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=RFH:(1E,3S,4R,5S,6R,7R,8R,9S,10S,11E,13E)-15-amino-1-{[(2S)-5,7-dihydroxy-2,4-dimethyl-8-{(E)-[(4-methylpiperazin-1-yl)imino]methyl}-1,6,9-trioxo-1,2,6,9-tetrahydronaphtho[2,1-b]furan-2-yl]oxy}-7,9-dihydroxy-3-methoxy-4,6,8,10,14-pentamethyl-15-oxopentadeca-1,11,13-trien-5-yl+acetate'>RFH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kow\|5kow]], [[5kox\|5kox]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kow\|5kow]], [[5kox\|5kox]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rox, NFA_35380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=247156 NOCFA])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c7s OCA], [http://pdbe.org/6c7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c7s RCSB], [http://www.ebi.ac.uk/pdbsum/6c7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c7s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c7s OCA], [http://pdbe.org/6c7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c7s RCSB], [http://www.ebi.ac.uk/pdbsum/6c7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c7s ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Nocfa]]
	[[Category: Liu, L K]]		[[Category: Liu, L K]]
	[[Category: Tanner, J J]]		[[Category: Tanner, J J]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Rifampicin]]		[[Category: Rifampicin]]

OCA at 05:44, 18 April 2018

OCA — Wed, 18 Apr 2018 05:44:43 GMT

←Older revision		Revision as of 05:44, 18 April 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6c7s is ~~ON HOLD until Paper~~ Publication	+	==Structure of Rifampicin Monooxygenase with Product Bound==
		+	<StructureSection load='6c7s' size='340' side='right' caption='[[6c7s]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6c7s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6C7S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6C7S FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=RFH:(1E,3S,4R,5S,6R,7R,8R,9S,10S,11E,13E)-15-amino-1-{[(2S)-5,7-dihydroxy-2,4-dimethyl-8-{(E)-[(4-methylpiperazin-1-yl)imino]methyl}-1,6,9-trioxo-1,2,6,9-tetrahydronaphtho[2,1-b]furan-2-yl]oxy}-7,9-dihydroxy-3-methoxy-4,6,8,10,14-pentamethyl-15-oxopentadeca-1,11,13-trien-5-yl+acetate'>RFH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5kow\|5kow]], [[5kox\|5kox]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6c7s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6c7s OCA], [http://pdbe.org/6c7s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6c7s RCSB], [http://www.ebi.ac.uk/pdbsum/6c7s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6c7s ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Rifampicin monooxygenase (RIFMO) decreases the potency of rifampicin (RIF) by converting it to oxidative products. Further decomposition of RIF has been observed in bacteria producing RIFMO and contributes to RIFMO-mediated drug resistance. Here we report the first crystal structure of RIFMO in complex with the hydroxylated RIF product. The 2.10 A resolution structure reveals a breach of the ansa aliphatic chain of RIF between naphthoquinone C2 and amide N1. Our data suggest that RIFMO catalyzes the hydroxylation of RIF at the C2 atom followed by cleavage of the ansa linkage, which leads to inactivation of the antibiotic by preventing key contacts with the RNA polymerase target.

-	~~Authors~~:	+	Structural Evidence for Rifampicin Monooxygenase Inactivating Rifampicin by Cleaving Its Ansa-Bridge.,Liu LK, Dai Y, Abdelwahab H, Sobrado P, Tanner JJ Biochemistry. 2018 Apr 10;57(14):2065-2068. doi: 10.1021/acs.biochem.8b00190., Epub 2018 Mar 30. PMID:29578336<ref>PMID:29578336</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6c7s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Liu, L K]]
		+	[[Category: Tanner, J J]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Rifampicin]]

OCA at 05:17, 15 February 2018

OCA — Thu, 15 Feb 2018 05:17:57 GMT

←Older revision		Revision as of 05:17, 15 February 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6c7s is ON HOLD	+	The entry 6c7s is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6c7s" [edit=sysop:move=sysop]

OCA — Wed, 31 Jan 2018 04:49:44 GMT

Protected "6c7s" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:49, 31 January 2018

OCA: New page: '''Unreleased structure''' The entry 6c7s is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 31 Jan 2018 04:49:42 GMT

New page: '''Unreleased structure''' The entry 6c7s is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6c7s is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]