6ccu - Revision history

OCA at 15:01, 4 October 2023

OCA — Wed, 04 Oct 2023 15:01:09 GMT

←Older revision		Revision as of 15:01, 4 October 2023
Line 1:		Line 1:

	==Complex between a GID4 fragment and a short peptide==		==Complex between a GID4 fragment and a short peptide==
-	<StructureSection load='6ccu' size='340' side='right' caption='[[6ccu]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='6ccu' size='340' side='right'caption='[[6ccu]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ccu]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CCU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ccu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CCU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~GID4, C17orf39, VID24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ccu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ccu OCA], [~~http~~://pdbe.org/6ccu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ccu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ccu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ccu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ccu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ccu OCA], [https://pdbe.org/6ccu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ccu RCSB], [https://www.ebi.ac.uk/pdbsum/6ccu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ccu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GID4_HUMAN GID4_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
-	[[Category: ~~Arrowsmith, C H~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Bountra, C~~]]	+	[[Category: Synthetic construct]]
-	[[Category: ~~Dong, C~~]]	+	[[Category: Arrowsmith CH]]
-	[[Category: ~~Edwards, A M~~]]	+	[[Category: Bountra C]]
-	[[Category: ~~Min, J~~]]	+	[[Category: Dong C]]
-	[[Category: ~~Structural genomic~~]]	+	[[Category: Edwards AM]]
-	[[Category: ~~Tempel, W~~]]	+	[[Category: Min J]]
-	[[Category: ~~Protein binding]]~~	+	[[Category: Tempel W]]
-	~~[[Category: Sgc~~]]	+

OCA at 06:44, 18 April 2018

OCA — Wed, 18 Apr 2018 06:44:48 GMT

←Older revision		Revision as of 06:44, 18 April 2018
Line 3:		Line 3:
	<StructureSection load='6ccu' size='340' side='right' caption='[[6ccu]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='6ccu' size='340' side='right' caption='[[6ccu]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ccu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CCU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ccu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CCU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GID4, C17orf39, VID24 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ccu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ccu OCA], [http://pdbe.org/6ccu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ccu RCSB], [http://www.ebi.ac.uk/pdbsum/6ccu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ccu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ccu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ccu OCA], [http://pdbe.org/6ccu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ccu RCSB], [http://www.ebi.ac.uk/pdbsum/6ccu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ccu ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The N-end rule pathway senses the N-terminal destabilizing residues of degradation substrates for the ubiquitin-proteasome system, whose integrity shields against various human syndromes including cancer and cardiovascular diseases. GID4, a subunit of the ubiquitin ligase GID complex, has been recently identified as the N-recognin of the new branch of the N-end rule pathway responsible for recognizing substrates bearing N-terminal proline residues (Pro/N-degrons). However, the molecular mechanism of GID4-mediated Pro/N-degron recognition remains largely unexplored. Here, we report the first crystal structures of human GID4 alone and in complex with various Pro/N-degrons. Our complex crystal structures, together with biophysical analyses, delineate the GID4-mediated Pro/N-degron recognition mechanism and substrate selection criteria for the Pro/N-end rule pathway. These mechanistic data on the Pro/N-recognin activity of GID4 will serve as a foundation to facilitate the identification of authentic physiological substrates as well as the development of inhibitors of therapeutic values for the Pro/N-end rule pathway.
		+
		+	Molecular basis of GID4-mediated recognition of degrons for the Pro/N-end rule pathway.,Dong C, Zhang H, Li L, Tempel W, Loppnau P, Min J Nat Chem Biol. 2018 May;14(5):466-473. doi: 10.1038/s41589-018-0036-1. Epub 2018 , Apr 9. PMID:29632410<ref>PMID:29632410</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ccu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Arrowsmith, C H]]		[[Category: Arrowsmith, C H]]
	[[Category: Bountra, C]]		[[Category: Bountra, C]]

OCA at 05:08, 8 March 2018

OCA — Thu, 08 Mar 2018 05:08:50 GMT

←Older revision		Revision as of 05:08, 8 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ccu is ~~ON HOLD~~	+	==Complex between a GID4 fragment and a short peptide==
-		+	<StructureSection load='6ccu' size='340' side='right' caption='[[6ccu]], [[Resolution\|resolution]] 1.75Å' scene=''>
-	~~Authors~~: ~~Dong, C~~.~~, Tempel, W~~.~~, Bountra, C~~., ~~Arrowsmith, C~~.H., ~~Edwards~~, A.M., ~~Min, J~~., ~~Structural Genomics Consortium (SGC)~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ccu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CCU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CCU FirstGlance]. <br>
-	~~Description: Complex between a GID4 fragment and a short peptide~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ccu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ccu OCA], [http://pdbe.org/6ccu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ccu RCSB], [http://www.ebi.ac.uk/pdbsum/6ccu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ccu ProSAT]</span></td></tr>
-	[[Category: ~~Arrowsmith~~, C.H]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Arrowsmith, C H]]
		+	[[Category: Bountra, C]]
	[[Category: Dong, C]]		[[Category: Dong, C]]
		+	[[Category: Edwards, A M]]
	[[Category: Min, J]]		[[Category: Min, J]]
-	~~[[Category: Edwards, A.M]]~~	+	[[Category: Structural genomic]]
-	[[Category: Structural ~~Genomics Consortium (Sgc)]]~~	+
-	~~[[Category: Bountra, C~~]]	+
	[[Category: Tempel, W]]		[[Category: Tempel, W]]
		+	[[Category: Protein binding]]
		+	[[Category: Sgc]]

OCA: Protected "6ccu" [edit=sysop:move=sysop]

OCA — Thu, 15 Feb 2018 05:23:28 GMT

Protected "6ccu" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:23, 15 February 2018

OCA: New page: '''Unreleased structure''' The entry 6ccu is ON HOLD Authors: Dong, C., Tempel, W., Bountra, C., Arrowsmith, C.H., Edwards, A.M., Min, J., Structural Genomics Consortium (SGC) Descript...

OCA — Thu, 15 Feb 2018 05:23:27 GMT

New page: '''Unreleased structure''' The entry 6ccu is ON HOLD Authors: Dong, C., Tempel, W., Bountra, C., Arrowsmith, C.H., Edwards, A.M., Min, J., Structural Genomics Consortium (SGC) Descript...

New page

'''Unreleased structure'''

The entry 6ccu is ON HOLD

Authors: Dong, C., Tempel, W., Bountra, C., Arrowsmith, C.H., Edwards, A.M., Min, J., Structural Genomics Consortium (SGC)

Description: Complex between a GID4 fragment and a short peptide
[[Category: Unreleased Structures]]
[[Category: Arrowsmith, C.H]]
[[Category: Dong, C]]
[[Category: Min, J]]
[[Category: Edwards, A.M]]
[[Category: Structural Genomics Consortium (Sgc)]]
[[Category: Bountra, C]]
[[Category: Tempel, W]]