6ceq - Revision history

OCA at 14:27, 13 March 2024

OCA — Wed, 13 Mar 2024 14:27:56 GMT

←Older revision		Revision as of 14:27, 13 March 2024
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	<StructureSection load='6ceq' size='340' side='right'caption='[[6ceq]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='6ceq' size='340' side='right'caption='[[6ceq]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ceq]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CEQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CEQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ceq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CEQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.67Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CSW01_19535 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~666~~ "~~Bacillo virgola del Koch~~" ~~Trevisan 1884])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ceq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ceq OCA], [~~http~~://pdbe.org/6ceq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ceq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ceq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ceq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ceq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ceq OCA], [https://pdbe.org/6ceq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ceq RCSB], [https://www.ebi.ac.uk/pdbsum/6ceq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ceq ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q9KKL2_VIBCH Q9KKL2_VIBCH]
-	The diarrheal pathogen Vibrio cholerae navigates complex environments using three chemosensory systems and 44-45 chemoreceptors. Chemosensory cluster II modulates chemotaxis, whereas clusters I and III have unknown functions. Ligands have been identified for only five V. cholerae chemoreceptors. Here we report that the cluster III receptor, VcAer2, binds and responds to O2 . VcAer2 is an ortholog of Pseudomonas aeruginosa Aer2 (PaAer2), but differs in that VcAer2 has two, rather than one, N-terminal PAS domain. We have determined that both PAS1 and PAS2 form homodimers and bind penta-coordinate b-type heme via an Eeta-His residue. Heme binding to PAS1 required the entire PAS core, but receptor function also required the N-terminal cap. PAS2 functioned as an O2 -sensor [Kd(O2) , 19 muM], utilizing the same Ibeta Trp (W276) as PaAer2 to stabilize O2 . The crystal structure of PAS2-W276L was similar to that of PaAer2-PAS, but resided in an active conformation mimicking the ligand-bound state, consistent with its signal-on phenotype. PAS1 also bound O2 [Kd(O2), 12 muM], although O2 binding was stabilized by either a Trp or Tyr residue. Moreover, PAS1 appeared to function as a signal modulator, regulating O2 -mediated signaling from PAS2, and resulting in activation of the cluster III chemosensory pathway. This article is protected by copyright. All rights reserved.	+
-		+
-	~~THE AER2 RECEPTOR FROM VIBRIO CHOLERAE IS A DUAL PAS-HEME OXYGEN SENSOR.,Greer-Phillips SE, Sukomon N, Chua TK, Johnson MS, Crane BR, Watts KJ Mol Microbiol. 2018 May 2. doi~~: ~~10.1111~~/~~mmi.13978. PMID:29719085<ref>PMID:29719085</ref>~~	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6ceq" style="background-color:#fffaf0;"><~~/~~div>~~	+

	==See Also==		==See Also==
	*[[Chemotaxis protein 3D structures\|Chemotaxis protein 3D structures]]		*[[Chemotaxis protein 3D structures\|Chemotaxis protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Chua, T K~~]]	+	[[Category: Vibrio cholerae]]
-	[[Category: ~~Crane, B R~~]]	+	[[Category: Chua TK]]
-	[[Category: ~~Sukomon, N~~]]	+	[[Category: Crane BR]]
-	[[Category: ~~Chemoreceptor]]~~	+	[[Category: Sukomon N]]
-	~~[[Category: Heme]]~~	+
-	~~[[Category: Pas domain]]~~	+
-	~~[[Category: Signal transduction]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Vibrio cholera~~]]	+

OCA at 10:44, 1 January 2020

OCA — Wed, 01 Jan 2020 10:44:28 GMT

←Older revision		Revision as of 10:44, 1 January 2020
Line 1:		Line 1:

	==The Aer2 Receptor from Vibrio cholerae is a Dual PAS-Heme Oxygen Sensor==		==The Aer2 Receptor from Vibrio cholerae is a Dual PAS-Heme Oxygen Sensor==
-	<StructureSection load='6ceq' size='340' side='right' caption='[[6ceq]], [[Resolution\|resolution]] 1.67Å' scene=''>	+	<StructureSection load='6ceq' size='340' side='right'caption='[[6ceq]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ceq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CEQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ceq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CEQ FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 6ceq" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6ceq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chemotaxis protein 3D structures\|Chemotaxis protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Bacillo virgola del koch trevisan 1884]]		[[Category: Bacillo virgola del koch trevisan 1884]]
		+	[[Category: Large Structures]]
	[[Category: Chua, T K]]		[[Category: Chua, T K]]
	[[Category: Crane, B R]]		[[Category: Crane, B R]]

OCA at 08:19, 21 February 2019

OCA — Thu, 21 Feb 2019 08:19:00 GMT

←Older revision		Revision as of 08:19, 21 February 2019
Line 3:		Line 3:
	<StructureSection load='6ceq' size='340' side='right' caption='[[6ceq]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='6ceq' size='340' side='right' caption='[[6ceq]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ceq]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CEQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ceq]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CEQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CSW01_19535 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ceq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ceq OCA], [http://pdbe.org/6ceq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ceq RCSB], [http://www.ebi.ac.uk/pdbsum/6ceq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ceq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ceq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ceq OCA], [http://pdbe.org/6ceq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ceq RCSB], [http://www.ebi.ac.uk/pdbsum/6ceq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ceq ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Chua, T K]]		[[Category: Chua, T K]]
	[[Category: Crane, B R]]		[[Category: Crane, B R]]

OCA at 05:31, 16 May 2018

OCA — Wed, 16 May 2018 05:31:18 GMT

←Older revision		Revision as of 05:31, 16 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6ceq is ~~ON HOLD until Paper~~ Publication	+	==The Aer2 Receptor from Vibrio cholerae is a Dual PAS-Heme Oxygen Sensor==
		+	<StructureSection load='6ceq' size='340' side='right' caption='[[6ceq]], [[Resolution\|resolution]] 1.67Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ceq]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CEQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ceq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ceq OCA], [http://pdbe.org/6ceq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ceq RCSB], [http://www.ebi.ac.uk/pdbsum/6ceq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ceq ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The diarrheal pathogen Vibrio cholerae navigates complex environments using three chemosensory systems and 44-45 chemoreceptors. Chemosensory cluster II modulates chemotaxis, whereas clusters I and III have unknown functions. Ligands have been identified for only five V. cholerae chemoreceptors. Here we report that the cluster III receptor, VcAer2, binds and responds to O2 . VcAer2 is an ortholog of Pseudomonas aeruginosa Aer2 (PaAer2), but differs in that VcAer2 has two, rather than one, N-terminal PAS domain. We have determined that both PAS1 and PAS2 form homodimers and bind penta-coordinate b-type heme via an Eeta-His residue. Heme binding to PAS1 required the entire PAS core, but receptor function also required the N-terminal cap. PAS2 functioned as an O2 -sensor [Kd(O2) , 19 muM], utilizing the same Ibeta Trp (W276) as PaAer2 to stabilize O2 . The crystal structure of PAS2-W276L was similar to that of PaAer2-PAS, but resided in an active conformation mimicking the ligand-bound state, consistent with its signal-on phenotype. PAS1 also bound O2 [Kd(O2), 12 muM], although O2 binding was stabilized by either a Trp or Tyr residue. Moreover, PAS1 appeared to function as a signal modulator, regulating O2 -mediated signaling from PAS2, and resulting in activation of the cluster III chemosensory pathway. This article is protected by copyright. All rights reserved.

-	~~Authors: Crane, B.R~~., Chua, ~~T.K.~~, ~~Sukomon~~, N.	+	THE AER2 RECEPTOR FROM VIBRIO CHOLERAE IS A DUAL PAS-HEME OXYGEN SENSOR.,Greer-Phillips SE, Sukomon N, Chua TK, Johnson MS, Crane BR, Watts KJ Mol Microbiol. 2018 May 2. doi: 10.1111/mmi.13978. PMID:29719085<ref>PMID:29719085</ref>

-	~~Description: The Aer2 Receptor from Vibrio cholerae is~~ a ~~Dual PAS~~-~~Heme Oxygen Sensor~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6ceq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chua, T K]]
		+	[[Category: Crane, B R]]
	[[Category: Sukomon, N]]		[[Category: Sukomon, N]]
-	[[Category: ~~Crane, B.R~~]]	+	[[Category: Chemoreceptor]]
-	[[Category: ~~Chua, T.K~~]]	+	[[Category: Heme]]
		+	[[Category: Pas domain]]
		+	[[Category: Signal transduction]]
		+	[[Category: Signaling protein]]
		+	[[Category: Vibrio cholera]]

OCA at 05:04, 14 March 2018

OCA — Wed, 14 Mar 2018 05:04:49 GMT

←Older revision		Revision as of 05:04, 14 March 2018
Line 5:		Line 5:
	Authors: Crane, B.R., Chua, T.K., Sukomon, N.		Authors: Crane, B.R., Chua, T.K., Sukomon, N.

-	Description: ~~Structural and functional analyses of PAS domains~~ from Vibrio ~~cholera aerotaxis receptor Aer2~~	+	Description: The Aer2 Receptor from Vibrio cholerae is a Dual PAS-Heme Oxygen Sensor
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Sukomon, N]]		[[Category: Sukomon, N]]
	[[Category: Crane, B.R]]		[[Category: Crane, B.R]]
	[[Category: Chua, T.K]]		[[Category: Chua, T.K]]

OCA: Protected "6ceq" [edit=sysop:move=sysop]

OCA — Thu, 08 Mar 2018 03:43:25 GMT

Protected "6ceq" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:43, 8 March 2018

OCA: New page: '''Unreleased structure''' The entry 6ceq is ON HOLD until Paper Publication Authors: Crane, B.R., Chua, T.K., Sukomon, N. Description: Structural and functional analyses of PAS domain...

OCA — Thu, 08 Mar 2018 03:43:24 GMT

New page: '''Unreleased structure''' The entry 6ceq is ON HOLD until Paper Publication Authors: Crane, B.R., Chua, T.K., Sukomon, N. Description: Structural and functional analyses of PAS domain...

New page

'''Unreleased structure'''

The entry 6ceq is ON HOLD until Paper Publication

Authors: Crane, B.R., Chua, T.K., Sukomon, N.

Description: Structural and functional analyses of PAS domains from Vibrio cholera aerotaxis receptor Aer2
[[Category: Unreleased Structures]]
[[Category: Sukomon, N]]
[[Category: Crane, B.R]]
[[Category: Chua, T.K]]