6cfc - Revision history

OCA at 15:03, 4 October 2023

OCA — Wed, 04 Oct 2023 15:03:01 GMT

←Older revision		Revision as of 15:03, 4 October 2023
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	==Crystal structure of soluble lytic transglycosylase Cj0843 of Campylobacter jejuni in complex with Bulgecin A==		==Crystal structure of soluble lytic transglycosylase Cj0843 of Campylobacter jejuni in complex with Bulgecin A==
-	<StructureSection load='6cfc' size='340' side='right' caption='[[6cfc]], [[Resolution\|resolution]] 2.04Å' scene=''>	+	<StructureSection load='6cfc' size='340' side='right'caption='[[6cfc]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cfc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp.~~ jejuni~~" smibert 1974~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CFC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cfc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CFC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BLG:4-O-(4-O-SULFONYL-N-ACETYLGLUCOSAMININYL)-5-METHYLHYDROXY-L-PROLINE-TAURINE'>BLG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.04Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BD28_04025 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLG:4-O-(4-O-SULFONYL-N-ACETYLGLUCOSAMININYL)-5-METHYLHYDROXY-L-PROLINE-TAURINE'>BLG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6cfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfc OCA], [~~http~~://pdbe.org/6cfc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6cfc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6cfc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfc OCA], [https://pdbe.org/6cfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cfc RCSB], [https://www.ebi.ac.uk/pdbsum/6cfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfc ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Campylobacter ~~fetus subsp.~~ jejuni ~~smibert 1974~~]]	+	[[Category: Campylobacter jejuni]]
-	[[Category: ~~Akker, F van den~~]]	+	[[Category: Large Structures]]
-	[[Category: Kumar, V]]	+	[[Category: Kumar V]]
-	[[Category: ~~Hydrolase~~]]	+	[[Category: Van den Akker F]]

OCA at 06:20, 6 June 2018

OCA — Wed, 06 Jun 2018 06:20:55 GMT

←Older revision		Revision as of 06:20, 6 June 2018
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-	==Crystal structure of soluble lytic transglycosylase Cj0843 of Campylobacter jejuni ~~at 2.3A resolution~~ in ~~I23 space group~~==	+	==Crystal structure of soluble lytic transglycosylase Cj0843 of Campylobacter jejuni in complex with Bulgecin A==
	<StructureSection load='6cfc' size='340' side='right' caption='[[6cfc]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='6cfc' size='340' side='right' caption='[[6cfc]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cfc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CFC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cfc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CFC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BLG:4-O-(4-O-SULFONYL-N-ACETYLGLUCOSAMININYL)-5-METHYLHYDROXY-L-PROLINE-TAURINE'>BLG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BLG:4-O-(4-O-SULFONYL-N-ACETYLGLUCOSAMININYL)-5-METHYLHYDROXY-L-PROLINE-TAURINE'>BLG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">BD28_04025 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfc OCA], [http://pdbe.org/6cfc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cfc RCSB], [http://www.ebi.ac.uk/pdbsum/6cfc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfc OCA], [http://pdbe.org/6cfc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cfc RCSB], [http://www.ebi.ac.uk/pdbsum/6cfc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfc ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Campylobacter fetus subsp. jejuni smibert 1974]]
	[[Category: Akker, F van den]]		[[Category: Akker, F van den]]
	[[Category: Kumar, V]]		[[Category: Kumar, V]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 05:34, 30 May 2018

OCA — Wed, 30 May 2018 05:34:48 GMT

←Older revision		Revision as of 05:34, 30 May 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6cfc is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of soluble lytic transglycosylase Cj0843 of Campylobacter jejuni at 2.3A resolution in I23 space group==
		+	<StructureSection load='6cfc' size='340' side='right' caption='[[6cfc]], [[Resolution\|resolution]] 2.04Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6cfc]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CFC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CFC FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BLG:4-O-(4-O-SULFONYL-N-ACETYLGLUCOSAMININYL)-5-METHYLHYDROXY-L-PROLINE-TAURINE'>BLG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cfc OCA], [http://pdbe.org/6cfc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cfc RCSB], [http://www.ebi.ac.uk/pdbsum/6cfc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cfc ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bacterial soluble lytic transglycosylase (LT) breaks down the peptidoglycan (PG) layer during processes such as cell division. We present here crystal structures of the soluble LT Cj0843 from Campylobacter jejuni with and without bulgecin A inhibitor in the active site. Cj0843 has a doughnut shape similar but not identical to that of E. coli SLT70. The C-terminal catalytic domain is preceded by an L-domain, a large helical U-domain, a flexible linker, and a small N-terminal NU-domain. The flexible linker allows the NU-domain to reach over and complete the circular shape, using residues conserved in the Epsilonproteobacteria LT family. The inner surface of the Cj0843 doughnut is mostly positively charged including a pocket that has 8 Arg/Lys residues. Molecular dynamics simulations with PG strands revealed a potential functional role for this pocket in anchoring the negatively charged terminal tetrapeptide of the PG during several steps in the reaction including homing and aligning the PG strand for exolytic cleavage, and subsequent ratcheting of the PG strand to enhance processivity in degrading PG strands.

-	~~Authors~~:	+	Structural studies and molecular dynamics simulations suggest a processive mechanism of exolytic lytic transglycosylase from Campylobacter jejuni.,Vijayaraghavan J, Kumar V, Krishnan NP, Kaufhold RT, Zeng X, Lin J, van den Akker F PLoS One. 2018 May 14;13(5):e0197136. doi: 10.1371/journal.pone.0197136., eCollection 2018. PMID:29758058<ref>PMID:29758058</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6cfc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Akker, F van den]]
		+	[[Category: Kumar, V]]
		+	[[Category: Hydrolase]]

OCA: Protected "6cfc" [edit=sysop:move=sysop]

OCA — Wed, 14 Mar 2018 05:05:10 GMT

Protected "6cfc" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:05, 14 March 2018

OCA: New page: '''Unreleased structure''' The entry 6cfc is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 14 Mar 2018 05:05:08 GMT

New page: '''Unreleased structure''' The entry 6cfc is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6cfc is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]