6ch5 - Revision history

OCA at 15:04, 4 October 2023

OCA — Wed, 04 Oct 2023 15:04:44 GMT

←Older revision		Revision as of 15:04, 4 October 2023
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	<StructureSection load='6ch5' size='340' side='right'caption='[[6ch5]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='6ch5' size='340' side='right'caption='[[6ch5]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ch5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CH5 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6CH5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ch5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Amphitrite_ornata Amphitrite ornata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CH5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CH5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F0M:2-methoxy-4-nitrophenol'>F0M</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ch5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ch5 OCA], [~~http~~://pdbe.org/6ch5 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ch5 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ch5 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ch5 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F0M:2-methoxy-4-nitrophenol'>F0M</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ch5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ch5 OCA], [https://pdbe.org/6ch5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ch5 RCSB], [https://www.ebi.ac.uk/pdbsum/6ch5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ch5 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9NAV7_9ANNE Q9NAV7_9ANNE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Amphitrite ornata]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Carey~~, L M~~]]	+	[[Category: Carey LM]]
-	[[Category: Ghiladi~~, R A~~]]	+	[[Category: Ghiladi RA]]
-	[[Category: Serrano~~, V S.de]]~~	+	[[Category: De Serrano VS]]
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 08:57, 11 November 2020

OCA — Wed, 11 Nov 2020 08:57:13 GMT

←Older revision		Revision as of 08:57, 11 November 2020
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	==Dehaloperoxidase B in complex with substrate 4-Nitroguaiacol==		==Dehaloperoxidase B in complex with substrate 4-Nitroguaiacol==
-	<StructureSection load='6ch5' size='340' side='right' caption='[[6ch5]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='6ch5' size='340' side='right'caption='[[6ch5]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ch5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CH5 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CH5 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ch5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CH5 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6CH5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F0M:2-methoxy-4-nitrophenol'>F0M</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F0M:2-methoxy-4-nitrophenol'>F0M</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ch5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ch5 OCA], [http://pdbe.org/6ch5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ch5 RCSB], [http://www.ebi.ac.uk/pdbsum/6ch5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ch5 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ch5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ch5 OCA], [http://pdbe.org/6ch5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ch5 RCSB], [http://www.ebi.ac.uk/pdbsum/6ch5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ch5 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 6ch5" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6ch5" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dehaloperoxidase 3D structures\|Dehaloperoxidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Carey, L M]]		[[Category: Carey, L M]]
	[[Category: Ghiladi, R A]]		[[Category: Ghiladi, R A]]
	[[Category: Serrano, V S.de]]		[[Category: Serrano, V S.de]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 12:04, 16 January 2019

OCA — Wed, 16 Jan 2019 12:04:54 GMT

←Older revision		Revision as of 12:04, 16 January 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6ch5 is ~~ON HOLD until Paper~~ Publication	+	==Dehaloperoxidase B in complex with substrate 4-Nitroguaiacol==
		+	<StructureSection load='6ch5' size='340' side='right' caption='[[6ch5]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ch5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CH5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CH5 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F0M:2-methoxy-4-nitrophenol'>F0M</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ch5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ch5 OCA], [http://pdbe.org/6ch5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ch5 RCSB], [http://www.ebi.ac.uk/pdbsum/6ch5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ch5 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The dehaloperoxidase-hemoglobin (DHP) from the terebellid polychaete Amphitrite ornata is a multifunctional hemoprotein that catalyzes the oxidation of a wide variety of substrates, including halo/nitrophenols, haloindoles, and pyrroles, via peroxidase and/or peroxygenase mechanisms. To probe whether substrate substituent effects can modulate enzyme activity in DHP, we investigated its reactiviy against a panel of o-guaiacol substrates given their presence (from native/halogenated and non-native/anthropogenic sources) in the benthic environment that A. ornata inhabits. Using biochemical assays supported by spectroscopic, spectrometric, and structural studies, DHP was found to catalyze the H2O2-dependent oxidative dehalogenation of 4-haloguaiacols (F, Cl, and Br) to 2-methoxybenzoquinone (2-MeOBQ). (18)O labeling studies confirmed that O atom incorporation was derived exclusively from water, consistent with substrate oxidation via a peroxidase-based mechanism. The 2-MeOBQ product further reduced DHP to its oxyferrous state, providing a link between the substrate oxidation and O2 carrier functions of DHP. Nonnative substrates resulted in polymerization of the initial substrate with varying degrees of oxidation, with 2-MeOBQ identified as a minor product. When viewed alongside the reactivity of previously studied phenolic substrates, the results presented here show that simple substituent effects can serve as functional switches between peroxidase and peroxygenase activities in this multifunctional catalytic globin. More broadly, when recent findings on DHP activity with nitrophenols and azoles are included, the results presented here further demonstrate the breadth of heterocyclic compounds of anthropogenic origin that can potentially disrupt marine hemoglobins or function as environmental stressors, findings that may be important when assessing the environmental impact of these pollutants (and their metabolites) on aquatic systems.

-	~~Authors~~:	+	Peroxidase versus Peroxygenase Activity: Substrate Substituent Effects as Modulators of Enzyme Function in the Multifunctional Catalytic Globin Dehaloperoxidase.,McGuire AH, Carey LM, de Serrano V, Dali S, Ghiladi RA Biochemistry. 2018 Jul 31;57(30):4455-4468. doi: 10.1021/acs.biochem.8b00540., Epub 2018 Jul 13. PMID:29949340<ref>PMID:29949340</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6ch5" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Carey, L M]]
		+	[[Category: Ghiladi, R A]]
		+	[[Category: Serrano, V S.de]]
		+	[[Category: Oxidoreductase]]

OCA at 05:05, 14 March 2018

OCA — Wed, 14 Mar 2018 05:05:48 GMT

←Older revision		Revision as of 05:05, 14 March 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ch5 is ON HOLD	+	The entry 6ch5 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6ch5" [edit=sysop:move=sysop]

OCA — Wed, 28 Feb 2018 05:05:03 GMT

Protected "6ch5" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:05, 28 February 2018

OCA: New page: '''Unreleased structure''' The entry 6ch5 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Feb 2018 05:05:02 GMT

New page: '''Unreleased structure''' The entry 6ch5 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ch5 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]