6chj - Revision history

OCA at 14:32, 6 March 2024

OCA — Wed, 06 Mar 2024 14:32:42 GMT

←Older revision		Revision as of 14:32, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='6chj' size='340' side='right'caption='[[6chj]], [[Resolution\|resolution]] 2.43Å' scene=''>		<StructureSection load='6chj' size='340' side='right'caption='[[6chj]], [[Resolution\|resolution]] 2.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6chj]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Marhv Marhv~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CHJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CHJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6chj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinobacter_nauticus_VT8 Marinobacter nauticus VT8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CHJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.428Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">Maqu_0168 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=351348 MARHV])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Diacylglycerol_O-acyltransferase Diacylglycerol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.20 2.3.1.20]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6chj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6chj OCA], [https://pdbe.org/6chj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6chj RCSB], [https://www.ebi.ac.uk/pdbsum/6chj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6chj ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6chj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6chj OCA], [~~http~~://pdbe.org/6chj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6chj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6chj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6chj ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A1TX06_MARN8 A1TX06_MARN8]
-	Wax esters are high-value products whose enzymatic synthesis is of increasing biotechnological interest. The fabrication of cell factories that mass-produce wax esters may provide a facile route towards a sustainable, and environment-friendly approach to a large-scale process for this commodity chemical. An expedient route for wax-ester biocatalysis may be facilitated by the action of enzymes termed wax ester synthases/diacylglycerol acyltransferases (WS/DGAT), which produce wax esters using fatty acids and alcohols as a precursor. In this work, we report the structure for a member of the WS/DGAT superfamily. The structural data in conjunction with bioinformatics and mutational analyses allowed us to identify the substrate binding pockets, and residues that may be important for catalysis. Using this information as a guide, we generated a mutant with preference towards shorter acyl-substrates. This study demonstrates the efficacy of a structure-guided engineering effort towards a WS/DGAT variant with preference towards wax esters of desired lengths.	+
-		+
-	~~Structural and Biochemical Studies of a Biocatalyst for the Enzymatic Production of Wax Esters.,Petronikolou N, Nair SK ACS Catal. 2018 Jul 6;8(7)~~:~~6334-6344. doi: 10.1021~~/~~acscatal.8b00787. Epub 2018, Jun 1. PMID:31559109<ref>PMID:31559109<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6chj" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Diacylglycerol O-acyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Marhv~~]]	+	[[Category: Marinobacter nauticus VT8]]
-	[[Category: Petronikolou, N]]	+	[[Category: Petronikolou N]]
-	[[Category: Satish~~, S K]]~~	+	[[Category: Satish SK]]
-	~~[[Category: Diacylglycerol acyltransferase]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Wax ester synthase]]~~	+
-	~~[[Category: Ws/dgat~~]]	+

OCA at 05:59, 10 October 2019

OCA — Thu, 10 Oct 2019 05:59:57 GMT

←Older revision		Revision as of 05:59, 10 October 2019
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	==Wax ester synthase/diacylglycerol acyltransferase from Marinobacter aquaeolei VT8==		==Wax ester synthase/diacylglycerol acyltransferase from Marinobacter aquaeolei VT8==
-	<StructureSection load='6chj' size='340' side='right' caption='[[6chj]], [[Resolution\|resolution]] 2.43Å' scene=''>	+	<StructureSection load='6chj' size='340' side='right'caption='[[6chj]], [[Resolution\|resolution]] 2.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6chj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CHJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6chj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Marhv Marhv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CHJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Maqu_0168 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=351348 MARHV])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diacylglycerol_O-acyltransferase Diacylglycerol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.20 2.3.1.20] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diacylglycerol_O-acyltransferase Diacylglycerol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.20 2.3.1.20] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6chj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6chj OCA], [http://pdbe.org/6chj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6chj RCSB], [http://www.ebi.ac.uk/pdbsum/6chj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6chj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6chj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6chj OCA], [http://pdbe.org/6chj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6chj RCSB], [http://www.ebi.ac.uk/pdbsum/6chj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6chj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Wax esters are high-value products whose enzymatic synthesis is of increasing biotechnological interest. The fabrication of cell factories that mass-produce wax esters may provide a facile route towards a sustainable, and environment-friendly approach to a large-scale process for this commodity chemical. An expedient route for wax-ester biocatalysis may be facilitated by the action of enzymes termed wax ester synthases/diacylglycerol acyltransferases (WS/DGAT), which produce wax esters using fatty acids and alcohols as a precursor. In this work, we report the structure for a member of the WS/DGAT superfamily. The structural data in conjunction with bioinformatics and mutational analyses allowed us to identify the substrate binding pockets, and residues that may be important for catalysis. Using this information as a guide, we generated a mutant with preference towards shorter acyl-substrates. This study demonstrates the efficacy of a structure-guided engineering effort towards a WS/DGAT variant with preference towards wax esters of desired lengths.
		+
		+	Structural and Biochemical Studies of a Biocatalyst for the Enzymatic Production of Wax Esters.,Petronikolou N, Nair SK ACS Catal. 2018 Jul 6;8(7):6334-6344. doi: 10.1021/acscatal.8b00787. Epub 2018, Jun 1. PMID:31559109<ref>PMID:31559109</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6chj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Diacylglycerol O-acyltransferase]]		[[Category: Diacylglycerol O-acyltransferase]]
		+	[[Category: Large Structures]]
		+	[[Category: Marhv]]
	[[Category: Petronikolou, N]]		[[Category: Petronikolou, N]]
	[[Category: Satish, S K]]		[[Category: Satish, S K]]

OCA at 07:38, 14 June 2018

OCA — Thu, 14 Jun 2018 07:38:28 GMT

←Older revision		Revision as of 07:38, 14 June 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6chj is ~~ON HOLD until Paper Publication~~	+	==Wax ester synthase/diacylglycerol acyltransferase from Marinobacter aquaeolei VT8==
-		+	<StructureSection load='6chj' size='340' side='right' caption='[[6chj]], [[Resolution\|resolution]] 2.43Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6chj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CHJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CHJ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diacylglycerol_O-acyltransferase Diacylglycerol O-acyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.20 2.3.1.20] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6chj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6chj OCA], [http://pdbe.org/6chj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6chj RCSB], [http://www.ebi.ac.uk/pdbsum/6chj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6chj ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Diacylglycerol O-acyltransferase]]
		+	[[Category: Petronikolou, N]]
		+	[[Category: Satish, S K]]
		+	[[Category: Diacylglycerol acyltransferase]]
		+	[[Category: Transferase]]
		+	[[Category: Wax ester synthase]]
		+	[[Category: Ws/dgat]]

OCA at 06:06, 21 March 2018

OCA — Wed, 21 Mar 2018 06:06:20 GMT

←Older revision		Revision as of 06:06, 21 March 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6chj is ON HOLD	+	The entry 6chj is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6chj" [edit=sysop:move=sysop]

OCA — Wed, 28 Feb 2018 05:05:56 GMT

Protected "6chj" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:05, 28 February 2018

OCA: New page: '''Unreleased structure''' The entry 6chj is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Feb 2018 05:05:55 GMT

New page: '''Unreleased structure''' The entry 6chj is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6chj is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]