6ci8 - Revision history

OCA at 15:05, 4 October 2023

OCA — Wed, 04 Oct 2023 15:05:17 GMT

←Older revision		Revision as of 15:05, 4 October 2023
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	<StructureSection load='6ci8' size='340' side='right'caption='[[6ci8]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6ci8' size='340' side='right'caption='[[6ci8]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ci8]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Mycs2 Mycs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CI8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CI8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ci8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CI8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CI8 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">MSMEG_0269 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=246196 MYCS2])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ci8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ci8 OCA], [https://pdbe.org/6ci8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ci8 RCSB], [https://www.ebi.ac.uk/pdbsum/6ci8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ci8 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ci8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ci8 OCA], [~~http~~://pdbe.org/6ci8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ci8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ci8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ci8 ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0QP46_MYCS2 A0QP46_MYCS2]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Mycs2~~]]	+	[[Category: Mycolicibacterium smegmatis MC2 155]]
-	[[Category: Kimber~~, M S~~]]	+	[[Category: Kimber MS]]
-	[[Category: Mallette, E]]	+	[[Category: Mallette E]]
-	~~[[Category: Dehydrogenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Short-chain dehydrogenase/reductase~~]]	+

OCA at 08:43, 8 January 2020

OCA — Wed, 08 Jan 2020 08:43:27 GMT

←Older revision		Revision as of 08:43, 8 January 2020
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	==Structure of the microcompartment-associated aminoacetone dehydrogenase==		==Structure of the microcompartment-associated aminoacetone dehydrogenase==
-	<StructureSection load='6ci8' size='340' side='right' caption='[[6ci8]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='6ci8' size='340' side='right'caption='[[6ci8]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ci8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CI8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CI8 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ci8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CI8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CI8 FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Mycs2]]		[[Category: Mycs2]]
	[[Category: Kimber, M S]]		[[Category: Kimber, M S]]

OCA at 06:12, 11 July 2018

OCA — Wed, 11 Jul 2018 06:12:25 GMT

←Older revision		Revision as of 06:12, 11 July 2018
Line 3:		Line 3:
	<StructureSection load='6ci8' size='340' side='right' caption='[[6ci8]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6ci8' size='340' side='right' caption='[[6ci8]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ci8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CI8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CI8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ci8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycs2 Mycs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CI8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CI8 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MSMEG_0269 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246196 MYCS2])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ci8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ci8 OCA], [http://pdbe.org/6ci8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ci8 RCSB], [http://www.ebi.ac.uk/pdbsum/6ci8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ci8 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ci8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ci8 OCA], [http://pdbe.org/6ci8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ci8 RCSB], [http://www.ebi.ac.uk/pdbsum/6ci8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ci8 ProSAT]</span></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Mycs2]]
	[[Category: Kimber, M S]]		[[Category: Kimber, M S]]
	[[Category: Mallette, E]]		[[Category: Mallette, E]]

OCA at 05:34, 30 May 2018

OCA — Wed, 30 May 2018 05:34:50 GMT

←Older revision		Revision as of 05:34, 30 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ci8 is ~~ON HOLD until Paper~~ Publication	+	==Structure of the microcompartment-associated aminoacetone dehydrogenase==
		+	<StructureSection load='6ci8' size='340' side='right' caption='[[6ci8]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ci8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CI8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CI8 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/3-oxoacyl-[acyl-carrier-protein]_reductase 3-oxoacyl-[acyl-carrier-protein] reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.100 1.1.1.100] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ci8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ci8 OCA], [http://pdbe.org/6ci8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ci8 RCSB], [http://www.ebi.ac.uk/pdbsum/6ci8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ci8 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	S-(+)-1-Amino-2-propanol dehydrogenase (APDH) is a short-chain dehydrogenase/reductase associated with the incompletely characterized Rhodococcus and Mycobacterium bacterial microcompartment (RMM). We enzymatically characterized the APDH from M. smegmatis and showed it is highly selective, with a low micromolar Km for S-(+)-1-amino-2-propanol and specificity for NADP(H). A paralogous enzyme from a nonmicrocompartment-associated operon in the same organism was also shown to have a similar activity. We determined the structure of APDH in both apo form (at 1.7 A) and as a ternary enzyme complex with NADP(+) and aminoacetone (at 1.9 A). Recognition of aminoacetone was mediated by strong hydrogen bonds to the amino group by Thr145 and by Glu251 from the C-terminus of an adjacent protomer. The substrate binding site entirely encloses the substrate, with close contacts between the aminoacetone methyl group and Phe95, Trp154, and Leu195. Kinetic characterization of several of these residues confirm their importance in enzyme functioning. Bioinformatics analysis of APDH homologues implies that many nonmicrocompartment APDH orthologues partake in an aminoacetone degradation pathway that proceeds via an aminopropanol O-phosphate phospholyase. RMM microcompartments may mediate a similar pathway, though possibly with differences in the details of the pathway that necessitates encapsulation behind a shell.

-	~~Authors: Mallette~~, E., Kimber~~, M~~.S.	+	Structure and Kinetics of the S-(+)-1-Amino-2-propanol Dehydrogenase from the RMM Microcompartment of Mycobacterium smegmatis.,Mallette E, Kimber MS Biochemistry. 2018 May 23. doi: 10.1021/acs.biochem.8b00464. PMID:29757625<ref>PMID:29757625</ref>

-	~~Description: Structure~~ of the ~~microcompartment~~-~~associated aminoacetone dehydrogenase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6ci8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Kimber, M S]]
	[[Category: Mallette, E]]		[[Category: Mallette, E]]
-	[[Category: ~~Kimber, M.S~~]]	+	[[Category: Dehydrogenase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Short-chain dehydrogenase/reductase]]

OCA at 04:37, 4 April 2018

OCA — Wed, 04 Apr 2018 04:37:38 GMT

←Older revision		Revision as of 04:37, 4 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ci8 is ON HOLD	+	The entry 6ci8 is ON HOLD until Paper Publication

	Authors: Mallette, E., Kimber, M.S.		Authors: Mallette, E., Kimber, M.S.

OCA: Protected "6ci8" [edit=sysop:move=sysop]

OCA — Thu, 08 Mar 2018 03:45:43 GMT

Protected "6ci8" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:45, 8 March 2018

OCA: New page: '''Unreleased structure''' The entry 6ci8 is ON HOLD Authors: Mallette, E., Kimber, M.S. Description: Structure of the microcompartment-associated aminoacetone dehydrogenase [[Category...

OCA — Thu, 08 Mar 2018 03:45:42 GMT

New page: '''Unreleased structure''' The entry 6ci8 is ON HOLD Authors: Mallette, E., Kimber, M.S. Description: Structure of the microcompartment-associated aminoacetone dehydrogenase [[Category...

New page

'''Unreleased structure'''

The entry 6ci8 is ON HOLD

Authors: Mallette, E., Kimber, M.S.

Description: Structure of the microcompartment-associated aminoacetone dehydrogenase
[[Category: Unreleased Structures]]
[[Category: Mallette, E]]
[[Category: Kimber, M.S]]