6crk - Revision history

OCA at 15:10, 4 October 2023

OCA — Wed, 04 Oct 2023 15:10:15 GMT

←Older revision		Revision as of 15:10, 4 October 2023
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	<StructureSection load='6crk' size='340' side='right'caption='[[6crk]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6crk' size='340' side='right'caption='[[6crk]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6crk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6CRK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6crk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CRK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6crk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crk OCA], [~~http~~://pdbe.org/6crk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6crk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6crk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6crk ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6crk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crk OCA], [https://pdbe.org/6crk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6crk RCSB], [https://www.ebi.ac.uk/pdbsum/6crk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6crk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GNAI1_HUMAN GNAI1_HUMAN]] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:17635935</ref> <ref>PMID:17264214</ref> [[http://www.uniprot.org/uniprot/GBG2_HUMAN GBG2_HUMAN]] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (By similarity). [[http://www.uniprot.org/uniprot/GBB1_HUMAN GBB1_HUMAN]] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.<ref>PMID:18611381</ref>	+	[https://www.uniprot.org/uniprot/GNAI1_HUMAN GNAI1_HUMAN] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:17635935</ref> <ref>PMID:17264214</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Dawson, R~~]]	+	[[Category: Mus musculus]]
-	[[Category: ~~Kobilka, B~~]]	+	[[Category: Dawson R]]
-	[[Category: ~~Maeda, S~~]]	+	[[Category: Kobilka B]]
-	[[Category: ~~Heterotrimeric g-protein antibody fragment]]~~	+	[[Category: Maeda S]]
-	~~[[Category: Signaling protein~~]]	+

OCA at 09:00, 11 November 2020

OCA — Wed, 11 Nov 2020 09:00:48 GMT

←Older revision		Revision as of 09:00, 11 November 2020
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	==Heterotrimeric G-protein in complex with an antibody fragment==		==Heterotrimeric G-protein in complex with an antibody fragment==
-	<StructureSection load='6crk' size='340' side='right' caption='[[6crk]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='6crk' size='340' side='right'caption='[[6crk]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6crk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CRK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6crk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6CRK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6crk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crk OCA], [http://pdbe.org/6crk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6crk RCSB], [http://www.ebi.ac.uk/pdbsum/6crk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6crk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6crk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crk OCA], [http://pdbe.org/6crk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6crk RCSB], [http://www.ebi.ac.uk/pdbsum/6crk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6crk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 6crk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6crk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transducin 3D structures\|Transducin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Dawson, R]]		[[Category: Dawson, R]]
	[[Category: Kobilka, B]]		[[Category: Kobilka, B]]

OCA at 05:52, 24 October 2018

OCA — Wed, 24 Oct 2018 05:52:20 GMT

←Older revision		Revision as of 05:52, 24 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6crk is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Heterotrimeric G-protein in complex with an antibody fragment==
		+	<StructureSection load='6crk' size='340' side='right' caption='[[6crk]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6crk]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CRK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6crk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crk OCA], [http://pdbe.org/6crk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6crk RCSB], [http://www.ebi.ac.uk/pdbsum/6crk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6crk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GNAI1_HUMAN GNAI1_HUMAN]] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:17635935</ref> <ref>PMID:17264214</ref> [[http://www.uniprot.org/uniprot/GBG2_HUMAN GBG2_HUMAN]] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction (By similarity). [[http://www.uniprot.org/uniprot/GBB1_HUMAN GBB1_HUMAN]] Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.<ref>PMID:18611381</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Single-particle cryo-electron microscopy (cryo-EM) has recently enabled high-resolution structure determination of numerous biological macromolecular complexes. Despite this progress, the application of high-resolution cryo-EM to G protein coupled receptors (GPCRs) in complex with heterotrimeric G proteins remains challenging, owning to both the relative small size and the limited stability of these assemblies. Here we describe the development of antibody fragments that bind and stabilize GPCR-G protein complexes for the application of high-resolution cryo-EM. One antibody in particular, mAb16, stabilizes GPCR/G-protein complexes by recognizing an interface between Galpha and Gbetagamma subunits in the heterotrimer, and confers resistance to GTPgammaS-triggered dissociation. The unique recognition mode of this antibody makes it possible to transfer its binding and stabilizing effect to other G-protein subtypes through minimal protein engineering. This antibody fragment is thus a broadly applicable tool for structural studies of GPCR/G-protein complexes.

-	~~Authors: Maeda~~, S., ~~Dawson~~, R., ~~Kobilka~~, B.	+	Development of an antibody fragment that stabilizes GPCR/G-protein complexes.,Maeda S, Koehl A, Matile H, Hu H, Hilger D, Schertler GFX, Manglik A, Skiniotis G, Dawson RJP, Kobilka BK Nat Commun. 2018 Sep 13;9(1):3712. doi: 10.1038/s41467-018-06002-w. PMID:30213947<ref>PMID:30213947</ref>

-	~~Description: Heterotrimeric G-protein in complex with an antibody fragment~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Maeda, S]]~~	+	<div class="pdbe-citations 6crk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Dawson, R]]		[[Category: Dawson, R]]
	[[Category: Kobilka, B]]		[[Category: Kobilka, B]]
		+	[[Category: Maeda, S]]
		+	[[Category: Heterotrimeric g-protein antibody fragment]]
		+	[[Category: Signaling protein]]

OCA at 04:39, 4 April 2018

OCA — Wed, 04 Apr 2018 04:39:17 GMT

←Older revision		Revision as of 04:39, 4 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6crk is ON HOLD	+	The entry 6crk is ON HOLD until Paper Publication

	Authors: Maeda, S., Dawson, R., Kobilka, B.		Authors: Maeda, S., Dawson, R., Kobilka, B.

OCA: Protected "6crk" [edit=sysop:move=sysop]

OCA — Wed, 28 Mar 2018 05:19:04 GMT

Protected "6crk" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:19, 28 March 2018

OCA: New page: '''Unreleased structure''' The entry 6crk is ON HOLD Authors: Maeda, S., Dawson, R., Kobilka, B. Description: Heterotrimeric G-protein in complex with an antibody fragment [[Category: ...

OCA — Wed, 28 Mar 2018 05:19:03 GMT

New page: '''Unreleased structure''' The entry 6crk is ON HOLD Authors: Maeda, S., Dawson, R., Kobilka, B. Description: Heterotrimeric G-protein in complex with an antibody fragment [[Category: ...

New page

'''Unreleased structure'''

The entry 6crk is ON HOLD

Authors: Maeda, S., Dawson, R., Kobilka, B.

Description: Heterotrimeric G-protein in complex with an antibody fragment
[[Category: Unreleased Structures]]
[[Category: Maeda, S]]
[[Category: Dawson, R]]
[[Category: Kobilka, B]]