6crw - Revision history

OCA at 23:48, 10 April 2020

OCA — Fri, 10 Apr 2020 23:48:44 GMT

←Older revision		Revision as of 23:48, 10 April 2020
Line 3:		Line 3:
	<SX load='6crw' size='340' side='right' viewer='molstar' caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>		<SX load='6crw' size='340' side='right' viewer='molstar' caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CRW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6CRW FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6crw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crw OCA], [http://pdbe.org/6crw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6crw RCSB], [http://www.ebi.ac.uk/pdbsum/6crw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6crw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6crw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crw OCA], [http://pdbe.org/6crw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6crw RCSB], [http://www.ebi.ac.uk/pdbsum/6crw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6crw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6crw" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6crw" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Spike protein\|Spike protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:47, 6 March 2020

OCA — Fri, 06 Mar 2020 20:47:50 GMT

←Older revision		Revision as of 20:47, 6 March 2020
Line 1:		Line 1:

	==SARS Spike Glycoprotein, Stabilized variant, single upwards S1 CTD conformation==		==SARS Spike Glycoprotein, Stabilized variant, single upwards S1 CTD conformation==
-	<~~StructureSection~~ load='6crw' size='340' side='right'caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>	+	<SX load='6crw' size='340' side='right' viewer='molstar' caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CRW FirstGlance]. <br>		<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CRW FirstGlance]. <br>
Line 22:		Line 22:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Bpt4]]		[[Category: Bpt4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 09:49, 18 December 2019

OCA — Wed, 18 Dec 2019 09:49:33 GMT

←Older revision		Revision as of 09:49, 18 December 2019
Line 1:		Line 1:

	==SARS Spike Glycoprotein, Stabilized variant, single upwards S1 CTD conformation==		==SARS Spike Glycoprotein, Stabilized variant, single upwards S1 CTD conformation==
-	<StructureSection load='6crw' size='340' side='right' caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>	+	<StructureSection load='6crw' size='340' side='right'caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CRW FirstGlance]. <br>		<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CRW FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Bpt4]]		[[Category: Bpt4]]
		+	[[Category: Large Structures]]
	[[Category: Cottrell, C A]]		[[Category: Cottrell, C A]]
	[[Category: Kirchdoerfer, R N]]		[[Category: Kirchdoerfer, R N]]

OCA at 07:11, 12 December 2018

OCA — Wed, 12 Dec 2018 07:11:51 GMT

←Older revision		Revision as of 07:11, 12 December 2018
Line 3:		Line 3:
	<StructureSection load='6crw' size='340' side='right' caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>		<StructureSection load='6crw' size='340' side='right' caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~9hiv1 9hiv1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CRW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CRW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~11676 9HIV1~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6crw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crw OCA], [http://pdbe.org/6crw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6crw RCSB], [http://www.ebi.ac.uk/pdbsum/6crw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6crw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6crw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crw OCA], [http://pdbe.org/6crw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6crw RCSB], [http://www.ebi.ac.uk/pdbsum/6crw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6crw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.		[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Severe acute respiratory syndrome coronavirus (SARS-CoV) emerged in 2002 as a highly transmissible pathogenic human betacoronavirus. The viral spike glycoprotein (S) utilizes angiotensin-converting enzyme 2 (ACE2) as a host protein receptor and mediates fusion of the viral and host membranes, making S essential to viral entry into host cells and host species tropism. As SARS-CoV enters host cells, the viral S is believed to undergo a number of conformational transitions as it is cleaved by host proteases and binds to host receptors. We recently developed stabilizing mutations for coronavirus spikes that prevent the transition from the pre-fusion to post-fusion states. Here, we present cryo-EM analyses of a stabilized trimeric SARS-CoV S, as well as the trypsin-cleaved, stabilized S, and its interactions with ACE2. Neither binding to ACE2 nor cleavage by trypsin at the S1/S2 cleavage site impart large conformational changes within stabilized SARS-CoV S or expose the secondary cleavage site, S2'.
		+
		+	Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis.,Kirchdoerfer RN, Wang N, Pallesen J, Wrapp D, Turner HL, Cottrell CA, Corbett KS, Graham BS, McLellan JS, Ward AB Sci Rep. 2018 Oct 24;8(1):15701. doi: 10.1038/s41598-018-34171-7. PMID:30356097<ref>PMID:30356097</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6crw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bpt4]]
	[[Category: Cottrell, C A]]		[[Category: Cottrell, C A]]
	[[Category: Kirchdoerfer, R N]]		[[Category: Kirchdoerfer, R N]]

OCA at 19:08, 1 August 2018

OCA — Wed, 01 Aug 2018 19:08:18 GMT

←Older revision		Revision as of 19:08, 1 August 2018
Line 3:		Line 3:
	<StructureSection load='6crw' size='340' side='right' caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>		<StructureSection load='6crw' size='340' side='right' caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CRW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CRW FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6crw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crw OCA], [http://pdbe.org/6crw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6crw RCSB], [http://www.ebi.ac.uk/pdbsum/6crw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6crw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6crw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crw OCA], [http://pdbe.org/6crw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6crw RCSB], [http://www.ebi.ac.uk/pdbsum/6crw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6crw ProSAT]</span></td></tr>
	</table>		</table>

OCA at 13:54, 11 April 2018

OCA — Wed, 11 Apr 2018 13:54:32 GMT

←Older revision		Revision as of 13:54, 11 April 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6crw is ~~ON HOLD~~	+	==SARS Spike Glycoprotein, Stabilized variant, single upwards S1 CTD conformation==
-		+	<StructureSection load='6crw' size='340' side='right' caption='[[6crw]], [[Resolution\|resolution]] 3.90Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6crw]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CRW FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6crw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6crw OCA], [http://pdbe.org/6crw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6crw RCSB], [http://www.ebi.ac.uk/pdbsum/6crw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6crw ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cottrell, C A]]
		+	[[Category: Kirchdoerfer, R N]]
		+	[[Category: McLellan, J S]]
		+	[[Category: Pallesen, J]]
		+	[[Category: Turner, H L]]
		+	[[Category: Wang, N]]
		+	[[Category: Ward, A B]]
		+	[[Category: Glycoprotein]]
		+	[[Category: Membrane fusion]]
		+	[[Category: Receptor binding]]
		+	[[Category: Viral protein]]

OCA: Protected "6crw" [edit=sysop:move=sysop]

OCA — Wed, 28 Mar 2018 05:19:52 GMT

Protected "6crw" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:19, 28 March 2018

OCA: New page: '''Unreleased structure''' The entry 6crw is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Mar 2018 05:19:51 GMT

New page: '''Unreleased structure''' The entry 6crw is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6crw is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]