6cuj - Revision history

OCA at 15:11, 4 October 2023

OCA — Wed, 04 Oct 2023 15:11:24 GMT

←Older revision		Revision as of 15:11, 4 October 2023
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	<StructureSection load='6cuj' size='340' side='right'caption='[[6cuj]], [[Resolution\|resolution]] 1.81Å' scene=''>		<StructureSection load='6cuj' size='340' side='right'caption='[[6cuj]], [[Resolution\|resolution]] 1.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cuj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CUJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6CUJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cuj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis Neisseria meningitidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CUJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6cuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cuj OCA], [~~http~~://pdbe.org/6cuj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6cuj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6cuj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6cuj ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.805Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cuj OCA], [https://pdbe.org/6cuj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cuj RCSB], [https://www.ebi.ac.uk/pdbsum/6cuj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cuj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NHBA_NEIME NHBA_NEIME] A major human immunogenic protein detected in patients recovering from meningitidis, where it induces bactericidal antibodies (PubMed:30133484) (By similarity). Binds human cells, heparin and heparan sulfate proteoglycan in vitro via the Arg-rich motif. Heparin-binding to this protein protects bacteria against killing by bactericidal antibodies (serum killing). The bacteria binds a number of human extracellular sialyated and/or sulfated glycans via this protein, including chondroitin sulfate, heparin and ganglioside GT3. Binds DNA non-specifically (By similarity).[UniProtKB:Q7DD37]<ref>PMID:30133484</ref> May play a role in extracellular-DNA (eDNA) mediated biofilm formation. When NHBA is not processed by NalP there is an increase in biofilm formation (PubMed:23163582). Lack of processing may lead to an increase in positively charged, NHBA- and IgA-derived DNA-binding peptides on the cell surface, resulting in increased DNA-binding peptides and increased biofilm formation (Probable).<ref>PMID:23163582</ref> <ref>PMID:23163582</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Malito, E~~]]	+	[[Category: Neisseria meningitidis]]
-	[[Category: ~~Spraggon, G~~]]	+	[[Category: Malito E]]
-	[[Category: ~~Disorder]]~~	+	[[Category: Spraggon G]]
-	~~[[Category: Lipid binding protein]]~~	+
-	~~[[Category: Meningitis]]~~	+
-	~~[[Category: Neisseria]]~~	+
-	~~[[Category: Vaccine~~]]	+

OCA at 09:01, 11 November 2020

OCA — Wed, 11 Nov 2020 09:01:10 GMT

←Older revision		Revision as of 09:01, 11 November 2020
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	==Crystal structure of the C-terminal domain of neisserial heparin binding antigen (NHBA)==		==Crystal structure of the C-terminal domain of neisserial heparin binding antigen (NHBA)==
-	<StructureSection load='6cuj' size='340' side='right' caption='[[6cuj]], [[Resolution\|resolution]] 1.81Å' scene=''>	+	<StructureSection load='6cuj' size='340' side='right'caption='[[6cuj]], [[Resolution\|resolution]] 1.81Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cuj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CUJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CUJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cuj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CUJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6CUJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6cuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cuj OCA], [http://pdbe.org/6cuj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cuj RCSB], [http://www.ebi.ac.uk/pdbsum/6cuj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cuj ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6cuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cuj OCA], [http://pdbe.org/6cuj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cuj RCSB], [http://www.ebi.ac.uk/pdbsum/6cuj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cuj ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Malito, E]]		[[Category: Malito, E]]
	[[Category: Spraggon, G]]		[[Category: Spraggon, G]]

OCA at 08:02, 26 September 2018

OCA — Wed, 26 Sep 2018 08:02:24 GMT

←Older revision		Revision as of 08:02, 26 September 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6cuj is ~~ON HOLD~~	+	==Crystal structure of the C-terminal domain of neisserial heparin binding antigen (NHBA)==
		+	<StructureSection load='6cuj' size='340' side='right' caption='[[6cuj]], [[Resolution\|resolution]] 1.81Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6cuj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CUJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CUJ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cuj OCA], [http://pdbe.org/6cuj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cuj RCSB], [http://www.ebi.ac.uk/pdbsum/6cuj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cuj ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Neisserial heparin binding antigen (NHBA) is one of three main recombinant protein antigens in 4CMenB, a vaccine for the prevention of invasive meningococcal disease caused by Neisseria meningitidis serogroup B. NHBA is a surface-exposed lipoprotein composed of a predicted disordered N-terminal region, an arginine-rich region that binds heparin, and a C-terminal domain that folds as an anti-parallel beta-barrel and that upon release after cleavage by human proteases alters endothelial permeability. NHBA induces bactericidal antibodies in humans, and NHBA-specific antibodies elicited by the 4CMenB vaccine contribute to serum bactericidal activity, the correlate of protection. To better understand the structural bases of the human antibody response to 4CMenB vaccination and to inform antigen design, we used X-ray crystallography to elucidate the structures of two C-terminal fragments of NHBA, either alone or in complex with the Fab derived from the vaccine-elicited human monoclonal antibody 5H2, and the structure of the unbound Fab 5H2. The structures reveal details on the interaction between an N-terminal beta-hairpin fragment and the beta-barrel, and explain how NHBA is capable of generating cross-reactive antibodies through an extensive conserved conformational epitope that covers the entire C-terminal face of the beta-barrel. By providing new structural information on a vaccine antigen and on the human immune response to vaccination, these results deepen our molecular understanding of 4CMenB, and might also aid future vaccine design projects.

-	~~Authors: Malito~~, E., ~~Spraggon~~, G.	+	Structures of NHBA elucidate a broadly conserved epitope identified by a vaccine induced antibody.,Maritan M, Veggi D, Cozzi R, Dello Iacono L, Bartolini E, Lo Surdo P, Maruggi G, Spraggon G, Bottomley MJ, Malito E PLoS One. 2018 Aug 22;13(8):e0201922. doi: 10.1371/journal.pone.0201922., eCollection 2018. PMID:30133484<ref>PMID:30133484</ref>

-	~~Description: Crystal structure~~ of the ~~C-terminal domain~~ of ~~neisserial heparin binding antigen (NHBA)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Spraggon, G]]~~	+	<div class="pdbe-citations 6cuj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Malito, E]]		[[Category: Malito, E]]
		+	[[Category: Spraggon, G]]
		+	[[Category: Disorder]]
		+	[[Category: Lipid binding protein]]
		+	[[Category: Meningitis]]
		+	[[Category: Neisseria]]
		+	[[Category: Vaccine]]

OCA: Protected "6cuj" [edit=sysop:move=sysop]

OCA — Wed, 04 Apr 2018 04:42:29 GMT

Protected "6cuj" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:42, 4 April 2018

OCA: New page: '''Unreleased structure''' The entry 6cuj is ON HOLD Authors: Malito, E., Spraggon, G. Description: Crystal structure of the C-terminal domain of neisserial heparin binding antigen (NH...

OCA — Wed, 04 Apr 2018 04:42:28 GMT

New page: '''Unreleased structure''' The entry 6cuj is ON HOLD Authors: Malito, E., Spraggon, G. Description: Crystal structure of the C-terminal domain of neisserial heparin binding antigen (NH...

New page

'''Unreleased structure'''

The entry 6cuj is ON HOLD

Authors: Malito, E., Spraggon, G.

Description: Crystal structure of the C-terminal domain of neisserial heparin binding antigen (NHBA)
[[Category: Unreleased Structures]]
[[Category: Spraggon, G]]
[[Category: Malito, E]]