6cza - Revision history

OCA at 14:31, 13 March 2024

OCA — Wed, 13 Mar 2024 14:31:34 GMT

←Older revision		Revision as of 14:31, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6cza' size='340' side='right'caption='[[6cza]], [[Resolution\|resolution]] 1.71Å' scene=''>		<StructureSection load='6cza' size='340' side='right'caption='[[6cza]], [[Resolution\|resolution]] 1.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cza]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~'shewanella_trabarsenatis' 'shewanella trabarsenatis'~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CZA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CZA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cza]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_sp._ANA-3 Shewanella sp. ANA-3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CZA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.71Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">arrA, Shewana3_2341 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=94122 'Shewanella trabarsenatis']), arrB, Shewana3_2340 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=94122 'Shewanella trabarsenatis'])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6cza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cza OCA], [~~http~~://pdbe.org/6cza PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6cza RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6cza PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6cza ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cza OCA], [https://pdbe.org/6cza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cza RCSB], [https://www.ebi.ac.uk/pdbsum/6cza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cza ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/ARRA_SHESA ARRA_SHESA] Component of the arsenate respiratory reductase (Arr) complex, which catalyzes the reduction of arsenate (As(V)) to arsenite (As(III)) (PubMed:12939408, PubMed:17951391, PubMed:30104376). ArrA is the arsenate-binding subunit (PubMed:30104376). The periplasmic localization of this complex may allow the cell to couple arsenate reduction to energy production before arsenate can be transported to the cell cytoplasm and enter the ars detoxification pathway, an energy-requiring process (PubMed:17951391).<ref>PMID:12939408</ref> <ref>PMID:17951391</ref> <ref>PMID:30104376</ref>
-	~~Arsenate respiration by bacteria was discovered over two decades ago and is catalyzed by diverse organisms using the well-conserved Arr enzyme complex~~. ~~Until now, the mechanisms underpinning this metabolism have been relatively opaque~~. ~~Here, we report~~ the ~~structure of an~~ Arr complex ~~(solved by X-ray crystallography to 1.6-A resolution)~~, which ~~was enabled by an improved Arr expression method in~~ the ~~genetically tractable arsenate respirer Shewanella sp. ANA-3. We also obtained structures bound with the substrate~~ arsenate (~~1.8 A), the product arsenite~~ (~~1.8 A~~)~~, and the natural inhibitor phosphate (1.7 A~~)~~. The structures reveal a conserved active-site motif that distinguishes Arr [(R/K)GRY] from the closely related~~ arsenite ~~respiratory oxidase~~ (~~Arx) complex~~ (~~XGRGWG~~)~~. Arr activity assays using methyl viologen as the electron donor and arsenate as the electron acceptor display two-site ping-pong kinetics. A Mo(V~~) ~~species was detected with EPR spectroscopy, which is typical for proteins with a pyranopterin guanine dinucleotide cofactor. Arr is an extraordinarily fast enzyme that approaches the diffusion limit~~ (~~Km = 44.6 +/- 1.6 muM~~, ~~kcat = 9~~,~~810 +/- 220 seconds(-1~~)~~), and phosphate~~ is ~~a competitive inhibitor of~~ arsenate ~~reduction~~ (~~Ki = 325 +/- 12 muM~~). ~~These observations, combined with knowledge~~ of ~~typical sedimentary arsenate and phosphate concentrations and known rates of arsenate desorption from minerals in~~ the ~~presence of phosphate, suggest that (i)~~ arsenate ~~desorption limits microbiologically induced~~ arsenate ~~reductive mobilization and (ii) phosphate enhances arsenic mobility by stimulating arsenate desorption rather than by inhibiting it at~~ the ~~enzymatic level.~~	+
-		+
-	~~Structural~~ and ~~mechanistic analysis of~~ the ~~arsenate respiratory reductase provides insight into environmental arsenic transformations.~~,~~Glasser NR, Oyala PH, Osborne TH, Santini JM, Newman DK Proc Natl Acad Sci U S A. 2018 Aug 13. pii~~: ~~1807984115~~. ~~doi:, 10.1073/pnas.1807984115. PMID:30104376~~<ref>PMID:~~30104376~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 6cza" style="background-color~~:~~#fffaf0;">~~</~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Shewanella trabarsenatis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Glasser, N R~~]]	+	[[Category: Shewanella sp. ANA-3]]
-	[[Category: ~~Newman, D K~~]]	+	[[Category: Glasser NR]]
-	[[Category: ~~Arsenate]]~~	+	[[Category: Newman DK]]
-	~~[[Category: Molybdoprotein]]~~	+
-	~~[[Category: Molybdopterin]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Phosphate~~]]	+

OCA at 10:01, 4 December 2019

OCA — Wed, 04 Dec 2019 10:01:23 GMT

←Older revision		Revision as of 10:01, 4 December 2019
Line 1:		Line 1:

	==The arsenate respiratory reductase (Arr) complex from Shewanella sp. ANA-3 bound to phosphate==		==The arsenate respiratory reductase (Arr) complex from Shewanella sp. ANA-3 bound to phosphate==
-	<StructureSection load='6cza' size='340' side='right' caption='[[6cza]], [[Resolution\|resolution]] 1.71Å' scene=''>	+	<StructureSection load='6cza' size='340' side='right'caption='[[6cza]], [[Resolution\|resolution]] 1.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6cza]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'shewanella_trabarsenatis' 'shewanella trabarsenatis']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CZA FirstGlance]. <br>		<table><tr><td colspan='2'>[[6cza]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'shewanella_trabarsenatis' 'shewanella trabarsenatis']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CZA FirstGlance]. <br>
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Shewanella trabarsenatis]]		[[Category: Shewanella trabarsenatis]]
		+	[[Category: Large Structures]]
	[[Category: Glasser, N R]]		[[Category: Glasser, N R]]
	[[Category: Newman, D K]]		[[Category: Newman, D K]]

OCA at 08:45, 3 October 2018

OCA — Wed, 03 Oct 2018 08:45:51 GMT

←Older revision		Revision as of 08:45, 3 October 2018
Line 5:		Line 5:
	<table><tr><td colspan='2'>[[6cza]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'shewanella_trabarsenatis' 'shewanella trabarsenatis']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CZA FirstGlance]. <br>		<table><tr><td colspan='2'>[[6cza]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'shewanella_trabarsenatis' 'shewanella trabarsenatis']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CZA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[d_1000233067\|d_1000233067]], [[d_1000233068\|d_1000233068]], [[d_1000233069\|d_1000233069]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">arrA, Shewana3_2341 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=94122 'Shewanella trabarsenatis']), arrB, Shewana3_2340 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=94122 'Shewanella trabarsenatis'])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">arrA, Shewana3_2341 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=94122 'Shewanella trabarsenatis']), arrB, Shewana3_2340 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=94122 'Shewanella trabarsenatis'])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cza OCA], [http://pdbe.org/6cza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cza RCSB], [http://www.ebi.ac.uk/pdbsum/6cza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cza ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cza OCA], [http://pdbe.org/6cza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cza RCSB], [http://www.ebi.ac.uk/pdbsum/6cza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cza ProSAT]</span></td></tr>

OCA at 07:59, 29 August 2018

OCA — Wed, 29 Aug 2018 07:59:33 GMT

←Older revision		Revision as of 07:59, 29 August 2018
Line 3:		Line 3:
	<StructureSection load='6cza' size='340' side='right' caption='[[6cza]], [[Resolution\|resolution]] 1.71Å' scene=''>		<StructureSection load='6cza' size='340' side='right' caption='[[6cza]], [[Resolution\|resolution]] 1.71Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6cza]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CZA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6cza]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/'shewanella_trabarsenatis' 'shewanella trabarsenatis']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CZA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[d_1000233067\|d_1000233067]], [[d_1000233068\|d_1000233068]], [[d_1000233069\|d_1000233069]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[d_1000233067\|d_1000233067]], [[d_1000233068\|d_1000233068]], [[d_1000233069\|d_1000233069]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">arrA, Shewana3_2341 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=94122 'Shewanella trabarsenatis']), arrB, Shewana3_2340 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=94122 'Shewanella trabarsenatis'])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cza OCA], [http://pdbe.org/6cza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cza RCSB], [http://www.ebi.ac.uk/pdbsum/6cza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cza ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cza OCA], [http://pdbe.org/6cza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cza RCSB], [http://www.ebi.ac.uk/pdbsum/6cza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cza ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Arsenate respiration by bacteria was discovered over two decades ago and is catalyzed by diverse organisms using the well-conserved Arr enzyme complex. Until now, the mechanisms underpinning this metabolism have been relatively opaque. Here, we report the structure of an Arr complex (solved by X-ray crystallography to 1.6-A resolution), which was enabled by an improved Arr expression method in the genetically tractable arsenate respirer Shewanella sp. ANA-3. We also obtained structures bound with the substrate arsenate (1.8 A), the product arsenite (1.8 A), and the natural inhibitor phosphate (1.7 A). The structures reveal a conserved active-site motif that distinguishes Arr [(R/K)GRY] from the closely related arsenite respiratory oxidase (Arx) complex (XGRGWG). Arr activity assays using methyl viologen as the electron donor and arsenate as the electron acceptor display two-site ping-pong kinetics. A Mo(V) species was detected with EPR spectroscopy, which is typical for proteins with a pyranopterin guanine dinucleotide cofactor. Arr is an extraordinarily fast enzyme that approaches the diffusion limit (Km = 44.6 +/- 1.6 muM, kcat = 9,810 +/- 220 seconds(-1)), and phosphate is a competitive inhibitor of arsenate reduction (Ki = 325 +/- 12 muM). These observations, combined with knowledge of typical sedimentary arsenate and phosphate concentrations and known rates of arsenate desorption from minerals in the presence of phosphate, suggest that (i) arsenate desorption limits microbiologically induced arsenate reductive mobilization and (ii) phosphate enhances arsenic mobility by stimulating arsenate desorption rather than by inhibiting it at the enzymatic level.
		+
		+	Structural and mechanistic analysis of the arsenate respiratory reductase provides insight into environmental arsenic transformations.,Glasser NR, Oyala PH, Osborne TH, Santini JM, Newman DK Proc Natl Acad Sci U S A. 2018 Aug 13. pii: 1807984115. doi:, 10.1073/pnas.1807984115. PMID:30104376<ref>PMID:30104376</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6cza" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Shewanella trabarsenatis]]
	[[Category: Glasser, N R]]		[[Category: Glasser, N R]]
	[[Category: Newman, D K]]		[[Category: Newman, D K]]

OCA at 16:15, 15 August 2018

OCA — Wed, 15 Aug 2018 16:15:07 GMT

←Older revision		Revision as of 16:15, 15 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6cza is ON HOLD until Paper Publication~~	+	==The arsenate respiratory reductase (Arr) complex from Shewanella sp. ANA-3 bound to phosphate==
-		+	<StructureSection load='6cza' size='340' side='right' caption='[[6cza]], [[Resolution\|resolution]] 1.71Å' scene=''>
-	~~Authors: Glasser, N.R., Newman, D.K.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6cza]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CZA FirstGlance]. <br>
-	~~Description:~~ The arsenate respiratory reductase (Arr) complex from Shewanella sp. ANA-3 bound to phosphate	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MGD:2-AMINO-5,6-DIMERCAPTO-7-METHYL-3,7,8A,9-TETRAHYDRO-8-OXA-1,3,9,10-TETRAAZA-ANTHRACEN-4-ONE+GUANOSINE+DINUCLEOTIDE'>MGD</scene>, <scene name='pdbligand=MO:MOLYBDENUM+ATOM'>MO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[d_1000233067\|d_1000233067]], [[d_1000233068\|d_1000233068]], [[d_1000233069\|d_1000233069]]</td></tr>
-	[[~~Category~~: ~~Newman~~, D.K]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cza OCA], [http://pdbe.org/6cza PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cza RCSB], [http://www.ebi.ac.uk/pdbsum/6cza PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cza ProSAT]</span></td></tr>
-	[[Category: Glasser, N.R]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Glasser, N R]]
		+	[[Category: Newman, D K]]
		+	[[Category: Arsenate]]
		+	[[Category: Molybdoprotein]]
		+	[[Category: Molybdopterin]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Phosphate]]

OCA: Protected "6cza" [edit=sysop:move=sysop]

OCA — Wed, 18 Apr 2018 04:15:01 GMT

Protected "6cza" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:15, 18 April 2018

OCA: New page: '''Unreleased structure''' The entry 6cza is ON HOLD until Paper Publication Authors: Glasser, N.R., Newman, D.K. Description: The arsenate respiratory reductase (Arr) complex from She...

OCA — Wed, 18 Apr 2018 04:15:00 GMT

New page: '''Unreleased structure''' The entry 6cza is ON HOLD until Paper Publication Authors: Glasser, N.R., Newman, D.K. Description: The arsenate respiratory reductase (Arr) complex from She...

New page

'''Unreleased structure'''

The entry 6cza is ON HOLD until Paper Publication

Authors: Glasser, N.R., Newman, D.K.

Description: The arsenate respiratory reductase (Arr) complex from Shewanella sp. ANA-3 bound to phosphate
[[Category: Unreleased Structures]]
[[Category: Newman, D.K]]
[[Category: Glasser, N.R]]