6d28 - Revision history

OCA at 14:32, 13 March 2024

OCA — Wed, 13 Mar 2024 14:32:12 GMT

←Older revision		Revision as of 14:32, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6d28' size='340' side='right'caption='[[6d28]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='6d28' size='340' side='right'caption='[[6d28]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6d28]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Canlf Canlf~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1qy5 1qy5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D28 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6D28 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6d28]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1qy5 1qy5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D28 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D28 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=M2M:1-METHOXY-2-(2-METHOXYETHOXY)ETHANE'>M2M</scene>, <scene name='pdbligand=NEC:N-ETHYL-5-CARBOXAMIDO+ADENOSINE'>NEC</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.75Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HSP90B1, GRP94, TRA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 CANLF])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M2M:1-METHOXY-2-(2-METHOXYETHOXY)ETHANE'>M2M</scene>, <scene name='pdbligand=NEC:N-ETHYL-5-CARBOXAMIDO+ADENOSINE'>NEC</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d28 OCA], [~~http~~://pdbe.org/6d28 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6d28 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6d28 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6d28 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d28 OCA], [https://pdbe.org/6d28 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d28 RCSB], [https://www.ebi.ac.uk/pdbsum/6d28 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d28 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/ENPL_CANLF ENPL_CANLF]] Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).	+	[https://www.uniprot.org/uniprot/ENPL_CANLF ENPL_CANLF] Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	GRP94, the endoplasmic reticulum (ER) paralog of the chaperone Hsp90, plays an essential role in the structural maturation or secretion of a subset of proteins destined for transport to the cell surface, such as the Toll-like receptors 2 and 4, and IgG, respectively. GRP94 differs from cytoplasmic Hsp90 by exhibiting very weak ATP binding and hydrolysis activity. GRP94 also binds selectively to a series of substituted adenosine analogs. The high resolution crystal structures at 1.75-2.1 A of the N-terminal and adjacent charged domains of GRP94 in complex with N-ethylcarboxamidoadenosine, radicicol, and 2-chlorodideoxyadenosine reveals a structural mechanism for ligand discrimination among hsp90 family members. The structures also identify a putative subdomain that may act as a ligand-responsive switch. The residues of the charged region fold into a disordered loop whose termini are ordered and continue the twisted beta sheet that forms the structural core of the N-domain. This continuation of the beta sheet past the charged domain suggests a structural basis for the association of the N-terminal and middle domains of the full-length chaperone.	+
-		+
-	Structure of the N-terminal domain of GRP94. Basis for ligand specificity and regulation.,Soldano KL, Jivan A, Nicchitta CV, Gewirth DT J Biol Chem. 2003 Nov 28;278(48):48330-8. Epub 2003 Sep 11. PMID:12970348<ref>PMID:12970348</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6d28" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Heat Shock Protein structures\|Heat Shock Protein structures]]		*[[Heat Shock Protein structures\|Heat Shock Protein structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Canlf~~]]	+	[[Category: Canis lupus familiaris]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gewirth~~, D T~~]]	+	[[Category: Gewirth DT]]
-	[[Category: Jivan, A]]	+	[[Category: Jivan A]]
-	[[Category: Nicchitta~~, C V~~]]	+	[[Category: Nicchitta CV]]
-	[[Category: Soldano~~, K L]]~~	+	[[Category: Soldano KL]]
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Grp94]]~~	+
-	~~[[Category: Hsp90]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Neca~~]]	+

OCA at 10:02, 4 December 2019

OCA — Wed, 04 Dec 2019 10:02:37 GMT

←Older revision		Revision as of 10:02, 4 December 2019
Line 21:		Line 21:

	==See Also==		==See Also==
-	*[[Heat Shock ~~Proteins~~\|Heat Shock ~~Proteins~~]]	+	*[[Heat Shock Protein structures\|Heat Shock Protein structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:29, 17 July 2019

OCA — Wed, 17 Jul 2019 12:29:12 GMT

←Older revision		Revision as of 12:29, 17 July 2019
Line 1:		Line 1:

	==Crystal Structure of the N-domain of the ER Hsp90 chaperone GRP94 in complex with the specific ligand NECA==		==Crystal Structure of the N-domain of the ER Hsp90 chaperone GRP94 in complex with the specific ligand NECA==
-	<StructureSection load='6d28' size='340' side='right' caption='[[6d28]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='6d28' size='340' side='right'caption='[[6d28]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6d28]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1qy5 1qy5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D28 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6d28]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canlf Canlf]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1qy5 1qy5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D28 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=M2M:1-METHOXY-2-(2-METHOXYETHOXY)ETHANE'>M2M</scene>, <scene name='pdbligand=NEC:N-ETHYL-5-CARBOXAMIDO+ADENOSINE'>NEC</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=M2M:1-METHOXY-2-(2-METHOXYETHOXY)ETHANE'>M2M</scene>, <scene name='pdbligand=NEC:N-ETHYL-5-CARBOXAMIDO+ADENOSINE'>NEC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">HSP90B1, GRP94, TRA1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9615 CANLF])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d28 OCA], [http://pdbe.org/6d28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d28 RCSB], [http://www.ebi.ac.uk/pdbsum/6d28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d28 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d28 OCA], [http://pdbe.org/6d28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d28 RCSB], [http://www.ebi.ac.uk/pdbsum/6d28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d28 ProSAT]</span></td></tr>
	</table>		</table>
Line 18:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6d28" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6d28" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Heat Shock Proteins\|Heat Shock Proteins]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Canlf]]
		+	[[Category: Large Structures]]
	[[Category: Gewirth, D T]]		[[Category: Gewirth, D T]]
	[[Category: Jivan, A]]		[[Category: Jivan, A]]

OCA at 05:14, 16 May 2018

OCA — Wed, 16 May 2018 05:14:47 GMT

←Older revision		Revision as of 05:14, 16 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6d28 is ~~ON HOLD~~	+	==Crystal Structure of the N-domain of the ER Hsp90 chaperone GRP94 in complex with the specific ligand NECA==
		+	<StructureSection load='6d28' size='340' side='right' caption='[[6d28]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6d28]] is a 1 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1qy5 1qy5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D28 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D28 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=M2M:1-METHOXY-2-(2-METHOXYETHOXY)ETHANE'>M2M</scene>, <scene name='pdbligand=NEC:N-ETHYL-5-CARBOXAMIDO+ADENOSINE'>NEC</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d28 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d28 OCA], [http://pdbe.org/6d28 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d28 RCSB], [http://www.ebi.ac.uk/pdbsum/6d28 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d28 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ENPL_CANLF ENPL_CANLF]] Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	GRP94, the endoplasmic reticulum (ER) paralog of the chaperone Hsp90, plays an essential role in the structural maturation or secretion of a subset of proteins destined for transport to the cell surface, such as the Toll-like receptors 2 and 4, and IgG, respectively. GRP94 differs from cytoplasmic Hsp90 by exhibiting very weak ATP binding and hydrolysis activity. GRP94 also binds selectively to a series of substituted adenosine analogs. The high resolution crystal structures at 1.75-2.1 A of the N-terminal and adjacent charged domains of GRP94 in complex with N-ethylcarboxamidoadenosine, radicicol, and 2-chlorodideoxyadenosine reveals a structural mechanism for ligand discrimination among hsp90 family members. The structures also identify a putative subdomain that may act as a ligand-responsive switch. The residues of the charged region fold into a disordered loop whose termini are ordered and continue the twisted beta sheet that forms the structural core of the N-domain. This continuation of the beta sheet past the charged domain suggests a structural basis for the association of the N-terminal and middle domains of the full-length chaperone.

-	~~Authors: Soldano, K~~.L., Jivan, A., Nicchitta, C.V.~~, Gewirth, D.T~~.	+	Structure of the N-terminal domain of GRP94. Basis for ligand specificity and regulation.,Soldano KL, Jivan A, Nicchitta CV, Gewirth DT J Biol Chem. 2003 Nov 28;278(48):48330-8. Epub 2003 Sep 11. PMID:12970348<ref>PMID:12970348</ref>

-	~~Description: Crystal Structure~~ of the ~~N-domain~~ of ~~the ER Hsp90 chaperone GRP94 in complex with the specific ligand NECA~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6d28" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gewirth, D T]]
	[[Category: Jivan, A]]		[[Category: Jivan, A]]
-	[[Category: Soldano, K.L]]	+	[[Category: Nicchitta, C V]]
-	[[Category: ~~Gewirth, D.T~~]]	+	[[Category: Soldano, K L]]
-	[[Category: ~~Nicchitta, C.V~~]]	+	[[Category: Chaperone]]
		+	[[Category: Grp94]]
		+	[[Category: Hsp90]]
		+	[[Category: Inhibitor]]
		+	[[Category: Neca]]

OCA: Protected "6d28" [edit=sysop:move=sysop]

OCA — Wed, 25 Apr 2018 04:05:30 GMT

Protected "6d28" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 25 April 2018

OCA: New page: '''Unreleased structure''' The entry 6d28 is ON HOLD Authors: Soldano, K.L., Jivan, A., Nicchitta, C.V., Gewirth, D.T. Description: Crystal Structure of the N-domain of the ER Hsp90 ch...

OCA — Wed, 25 Apr 2018 04:05:29 GMT

New page: '''Unreleased structure''' The entry 6d28 is ON HOLD Authors: Soldano, K.L., Jivan, A., Nicchitta, C.V., Gewirth, D.T. Description: Crystal Structure of the N-domain of the ER Hsp90 ch...

New page

'''Unreleased structure'''

The entry 6d28 is ON HOLD

Authors: Soldano, K.L., Jivan, A., Nicchitta, C.V., Gewirth, D.T.

Description: Crystal Structure of the N-domain of the ER Hsp90 chaperone GRP94 in complex with the specific ligand NECA
[[Category: Unreleased Structures]]
[[Category: Jivan, A]]
[[Category: Soldano, K.L]]
[[Category: Gewirth, D.T]]
[[Category: Nicchitta, C.V]]