6d38 - Revision history

OCA at 15:17, 4 October 2023

OCA — Wed, 04 Oct 2023 15:17:03 GMT

←Older revision

Revision as of 15:17, 4 October 2023

OCA at 10:55, 1 January 2020

OCA — Wed, 01 Jan 2020 10:55:22 GMT

←Older revision		Revision as of 10:55, 1 January 2020
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	==Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)==		==Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)==
-	<StructureSection load='6d38' size='340' side='right' caption='[[6d38]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='6d38' size='340' side='right'caption='[[6d38]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6d38]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D38 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6d38]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Echinophyllia_sp._sc22 Echinophyllia sp. sc22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D38 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6d39\|6d39]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6d39\|6d39]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Dronpa ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=301887 Echinophyllia sp. SC22])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d38 OCA], [http://pdbe.org/6d38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d38 RCSB], [http://www.ebi.ac.uk/pdbsum/6d38 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d38 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d38 OCA], [http://pdbe.org/6d38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d38 RCSB], [http://www.ebi.ac.uk/pdbsum/6d38 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d38 ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 6d38" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6d38" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dronpa\|Dronpa]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Echinophyllia sp. sc22]]
		+	[[Category: Large Structures]]
	[[Category: Fan, L]]		[[Category: Fan, L]]
	[[Category: Lin, M]]		[[Category: Lin, M]]

OCA at 05:33, 16 May 2018

OCA — Wed, 16 May 2018 05:33:17 GMT

←Older revision		Revision as of 05:33, 16 May 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6d38 is ~~ON HOLD~~	+	==Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)==
		+	<StructureSection load='6d38' size='340' side='right' caption='[[6d38]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6d38]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D38 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6d39\|6d39]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d38 OCA], [http://pdbe.org/6d38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d38 RCSB], [http://www.ebi.ac.uk/pdbsum/6d38 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d38 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein kinases transduce signals to regulate a wide array of cellular functions in eukaryotes. A generalizable method for optical control of kinases would enable fine spatiotemporal interrogation or manipulation of these various functions. We report the design and application of single-chain cofactor-free kinases with photoswitchable activity. We engineered a dimeric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light. Attaching two pdDronpa domains at rationally selected locations in the kinase domain, we created the photoswitchable kinases psRaf1, psMEK1, psMEK2, and psCDK5. Using these photoswitchable kinases, we established an all-optical cell-based assay for screening inhibitors, uncovered a direct and rapid inhibitory feedback loop from ERK to MEK1, and mediated developmental changes and synaptic vesicle transport in vivo using light.

-	~~Authors: Zhou, X~~., Fan, L., ~~Lin~~, M.	+	Optical control of cell signaling by single-chain photoswitchable kinases.,Zhou XX, Fan LZ, Li P, Shen K, Lin MZ Science. 2017 Feb 24;355(6327):836-842. doi: 10.1126/science.aah3605. PMID:28232577<ref>PMID:28232577</ref>

-	~~Description: Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Zhou, X]]~~	+	<div class="pdbe-citations 6d38" style="background-color:#fffaf0;"></div>
-	~~[[Category: Lin, M]]~~	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Fan, L]]		[[Category: Fan, L]]
		+	[[Category: Lin, M]]
		+	[[Category: Zhou, X]]
		+	[[Category: Dimer]]
		+	[[Category: Dronpa]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Gfp]]
		+	[[Category: Optogenetic]]
		+	[[Category: Pddronpa]]
		+	[[Category: Photodissociable]]

OCA: Protected "6d38" [edit=sysop:move=sysop]

OCA — Wed, 25 Apr 2018 04:08:10 GMT

Protected "6d38" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:08, 25 April 2018

OCA: New page: '''Unreleased structure''' The entry 6d38 is ON HOLD Authors: Zhou, X., Fan, L., Lin, M. Description: Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM) [[...

OCA — Wed, 25 Apr 2018 04:08:09 GMT

New page: '''Unreleased structure''' The entry 6d38 is ON HOLD Authors: Zhou, X., Fan, L., Lin, M. Description: Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM) [[...

New page

'''Unreleased structure'''

The entry 6d38 is ON HOLD

Authors: Zhou, X., Fan, L., Lin, M.

Description: Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)
[[Category: Unreleased Structures]]
[[Category: Zhou, X]]
[[Category: Lin, M]]
[[Category: Fan, L]]