6d8o - Revision history

OCA at 15:19, 4 October 2023

OCA — Wed, 04 Oct 2023 15:19:58 GMT

←Older revision		Revision as of 15:19, 4 October 2023
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	<StructureSection load='6d8o' size='340' side='right'caption='[[6d8o]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='6d8o' size='340' side='right'caption='[[6d8o]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6d8o]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D8O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6D8O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6d8o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D8O FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8011248Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6d8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d8o OCA], [~~http~~://pdbe.org/6d8o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6d8o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6d8o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6d8o ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d8o OCA], [https://pdbe.org/6d8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d8o RCSB], [https://www.ebi.ac.uk/pdbsum/6d8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d8o ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	==See Also==		==See Also==
-	*[[Ribozyme\|Ribozyme]]	+	*[[Ribozyme 3D structures\|Ribozyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Shoffner, G M~~]]	+	[[Category: Tetrahymena thermophila]]
-	[[Category: ~~Ribozyme]]~~	+	[[Category: Shoffner GM]]
-	~~[[Category: Rna~~]]	+

OCA at 09:04, 11 November 2020

OCA — Wed, 11 Nov 2020 09:04:25 GMT

←Older revision		Revision as of 09:04, 11 November 2020
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	==Group I self-splicing intron P4-P6 domain mutant A230U==		==Group I self-splicing intron P4-P6 domain mutant A230U==
-	<StructureSection load='6d8o' size='340' side='right' caption='[[6d8o]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='6d8o' size='340' side='right'caption='[[6d8o]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6d8o]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D8O OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6D8O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6d8o]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D8O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6D8O FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6d8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d8o OCA], [http://pdbe.org/6d8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d8o RCSB], [http://www.ebi.ac.uk/pdbsum/6d8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d8o ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6d8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d8o OCA], [http://pdbe.org/6d8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d8o RCSB], [http://www.ebi.ac.uk/pdbsum/6d8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d8o ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Shoffner, G M]]		[[Category: Shoffner, G M]]
	[[Category: Ribozyme]]		[[Category: Ribozyme]]
	[[Category: Rna]]		[[Category: Rna]]

OCA at 20:18, 19 September 2018

OCA — Wed, 19 Sep 2018 20:18:47 GMT

←Older revision		Revision as of 20:18, 19 September 2018
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	</div>		</div>
	<div class="pdbe-citations 6d8o" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6d8o" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ribozyme\|Ribozyme]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:14, 4 July 2018

OCA — Wed, 04 Jul 2018 07:14:57 GMT

←Older revision		Revision as of 07:14, 4 July 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6d8o is ~~ON HOLD until Paper~~ Publication	+	==Group I self-splicing intron P4-P6 domain mutant A230U==
		+	<StructureSection load='6d8o' size='340' side='right' caption='[[6d8o]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6d8o]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D8O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D8O FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d8o OCA], [http://pdbe.org/6d8o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d8o RCSB], [http://www.ebi.ac.uk/pdbsum/6d8o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d8o ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Crystallography is a major technique for determining large RNA structures. Obtaining diffraction-quality crystals has been the bottleneck. Although several RNA crystallization methods have been developed, the field strongly needs additional approaches. Here we invented an in crystallo selection strategy for identifying mutations that enhance a target RNA's crystallizability. The strategy includes constructing an RNA pool containing random mutations, obtaining crystals, and amplifying the sequences enriched by crystallization. We demonstrated a proof-of-principle application to the P4-P6 domain from the Tetrahymena ribozyme. We further determined the structures of four selected mutants. All four establish new crystal lattice contacts while maintaining the native structure. Three mutants achieve this by relocating bulges and one by making a helix more flexible. In crystallo selection provides opportunities to improve crystals of RNAs or RNA-ligand complexes. Our results also suggest that mutants may be rationally designed for crystallization by "walking" a bulge along the RNA chain.

-	~~Authors:~~ Shoffner, G.M.	+	In Crystallo Selection to Establish New RNA Crystal Contacts.,Shoffner GM, Wang R, Podell E, Cech TR, Guo F Structure. 2018 May 28. pii: S0969-2126(18)30169-2. doi:, 10.1016/j.str.2018.05.005. PMID:29910185<ref>PMID:29910185</ref>

-	~~Description: Group I self~~-~~splicing intron P4~~-~~P6 domain mutant A230U~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Shoffner, G.M~~]]	+	<div class="pdbe-citations 6d8o" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Shoffner, G M]]
		+	[[Category: Ribozyme]]
		+	[[Category: Rna]]

OCA at 04:02, 30 May 2018

OCA — Wed, 30 May 2018 04:02:05 GMT

←Older revision		Revision as of 04:02, 30 May 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6d8o is ON HOLD	+	The entry 6d8o is ON HOLD until Paper Publication

	Authors: Shoffner, G.M.		Authors: Shoffner, G.M.

OCA: Protected "6d8o" [edit=sysop:move=sysop]

OCA — Wed, 02 May 2018 06:50:16 GMT

Protected "6d8o" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:50, 2 May 2018

OCA: New page: '''Unreleased structure''' The entry 6d8o is ON HOLD Authors: Shoffner, G.M. Description: Group I self-splicing intron P4-P6 domain mutant A230U Category: Unreleased Structures [[C...

OCA — Wed, 02 May 2018 06:50:14 GMT

New page: '''Unreleased structure''' The entry 6d8o is ON HOLD Authors: Shoffner, G.M. Description: Group I self-splicing intron P4-P6 domain mutant A230U Category: Unreleased Structures [[C...

New page

'''Unreleased structure'''

The entry 6d8o is ON HOLD

Authors: Shoffner, G.M.

Description: Group I self-splicing intron P4-P6 domain mutant A230U
[[Category: Unreleased Structures]]
[[Category: Shoffner, G.M]]