6dcs - Revision history

OCA at 14:33, 13 March 2024

OCA — Wed, 13 Mar 2024 14:33:46 GMT

←Older revision		Revision as of 14:33, 13 March 2024
Line 1:		Line 1:

	==Stage III sporulation protein AF (SpoIIIAF)==		==Stage III sporulation protein AF (SpoIIIAF)==
-	<StructureSection load='6dcs' size='340' side='right' caption='[[6dcs]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='6dcs' size='340' side='right'caption='[[6dcs]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dcs]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DCS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6DCS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dcs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DCS FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~spoIIIAF, BSU24380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=224308 BACSU])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6dcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dcs OCA], [~~http~~://pdbe.org/6dcs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6dcs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6dcs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6dcs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dcs OCA], [https://pdbe.org/6dcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dcs RCSB], [https://www.ebi.ac.uk/pdbsum/6dcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dcs ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/SP3AF_BACSU SP3AF_BACSU]
-	Environmental stress factors initiate the developmental process of sporulation in some Gram-positive bacteria including Bacillus subtilis. Upon sporulation initiation the bacterial cell undergoes a series of morphological transformations that result in the creation of a single dormant spore. Early in sporulation, an asymmetric cell division produces a larger mother cell and smaller forespore. Next, the mother cell septal membrane engulfs the forespore, and an essential channel, the so-called feeding-tube apparatus, is formed. This assembled channel is thought to form a transenvelope secretion complex that crosses both mother cell and forespore membranes. At least nine proteins are essential for channel formation including SpoIIQ under forespore control and the eight SpoIIIA proteins (SpoIIIAA-AH) under mother cell control. Several of these proteins share similarity with components of Gram-negative bacterial secretion systems and the flagellum. Here we report the X-ray crystallographic structure of the soluble domain of SpoIIIAF to 2.7A resolution. Like the channel components SpoIIIAG and SpoIIIAH, SpoIIIAF adopts a conserved ring-building motif (RBM) fold found in proteins from numerous dual membrane secretion systems of distinct function. The SpoIIIAF RBM fold contains two unique features: an extended N-terminal helix, associated with multimerization, and an insertion at a loop region that can adopt two distinct conformations. The ability of the same primary sequence to adopt different secondary structure conformations is associated with protein regulation, suggesting a dual structural and regulatory role for the SpoIIIAF RBM. We further analyzed potential interaction interfaces by structure-guided mutagenesis in vivo. Collectively, our data provide new insight into the possible roles of SpoIIIAF within the secretion-like apparatus during sporulation.	+
-		+
-	Structural and biochemical characterization of SpoIIIAF, a component of a sporulation-essential channel in Bacillus subtilis.,Zeytuni N, Flanagan KA, Worrall LJ, Massoni SC, Camp AH, Strynadka NCJ J Struct Biol. 2018 Jun 7. pii: S1047-8477(18)30140-0. doi:, 10.1016/~~j.jsb.2018.06.002. PMID:29886194<ref>PMID:29886194<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6dcs" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacsu~~]]	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
-	[[Category: ~~Camp, A H~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Flanagan, K A~~]]	+	[[Category: Camp AH]]
-	[[Category: ~~Strynadka, N C.J~~]]	+	[[Category: Flanagan KA]]
-	[[Category: ~~Zeytuni, N~~]]	+	[[Category: Strynadka NCJ]]
-	[[Category: ~~Protein transport]]~~	+	[[Category: Zeytuni N]]
-	~~[[Category: Secretion system]]~~	+
-	~~[[Category: Sporulation~~]]	+

OCA at 07:52, 12 September 2018

OCA — Wed, 12 Sep 2018 07:52:16 GMT

←Older revision		Revision as of 07:52, 12 September 2018
Line 3:		Line 3:
	<StructureSection load='6dcs' size='340' side='right' caption='[[6dcs]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='6dcs' size='340' side='right' caption='[[6dcs]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dcs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DCS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dcs]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DCS FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">spoIIIAF, BSU24380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dcs OCA], [http://pdbe.org/6dcs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dcs RCSB], [http://www.ebi.ac.uk/pdbsum/6dcs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dcs ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dcs OCA], [http://pdbe.org/6dcs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dcs RCSB], [http://www.ebi.ac.uk/pdbsum/6dcs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dcs ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacsu]]
	[[Category: Camp, A H]]		[[Category: Camp, A H]]
	[[Category: Flanagan, K A]]		[[Category: Flanagan, K A]]

OCA at 07:38, 18 July 2018

OCA — Wed, 18 Jul 2018 07:38:26 GMT

←Older revision		Revision as of 07:38, 18 July 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6dcs is ~~ON HOLD until Paper~~ Publication	+	==Stage III sporulation protein AF (SpoIIIAF)==
		+	<StructureSection load='6dcs' size='340' side='right' caption='[[6dcs]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6dcs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DCS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DCS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dcs OCA], [http://pdbe.org/6dcs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dcs RCSB], [http://www.ebi.ac.uk/pdbsum/6dcs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dcs ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Environmental stress factors initiate the developmental process of sporulation in some Gram-positive bacteria including Bacillus subtilis. Upon sporulation initiation the bacterial cell undergoes a series of morphological transformations that result in the creation of a single dormant spore. Early in sporulation, an asymmetric cell division produces a larger mother cell and smaller forespore. Next, the mother cell septal membrane engulfs the forespore, and an essential channel, the so-called feeding-tube apparatus, is formed. This assembled channel is thought to form a transenvelope secretion complex that crosses both mother cell and forespore membranes. At least nine proteins are essential for channel formation including SpoIIQ under forespore control and the eight SpoIIIA proteins (SpoIIIAA-AH) under mother cell control. Several of these proteins share similarity with components of Gram-negative bacterial secretion systems and the flagellum. Here we report the X-ray crystallographic structure of the soluble domain of SpoIIIAF to 2.7A resolution. Like the channel components SpoIIIAG and SpoIIIAH, SpoIIIAF adopts a conserved ring-building motif (RBM) fold found in proteins from numerous dual membrane secretion systems of distinct function. The SpoIIIAF RBM fold contains two unique features: an extended N-terminal helix, associated with multimerization, and an insertion at a loop region that can adopt two distinct conformations. The ability of the same primary sequence to adopt different secondary structure conformations is associated with protein regulation, suggesting a dual structural and regulatory role for the SpoIIIAF RBM. We further analyzed potential interaction interfaces by structure-guided mutagenesis in vivo. Collectively, our data provide new insight into the possible roles of SpoIIIAF within the secretion-like apparatus during sporulation.

-	~~Authors: Strynadka~~, ~~N.C.J~~., Zeytuni, N., Camp, A.H., ~~Flanagan, K~~.A.	+	Structural and biochemical characterization of SpoIIIAF, a component of a sporulation-essential channel in Bacillus subtilis.,Zeytuni N, Flanagan KA, Worrall LJ, Massoni SC, Camp AH, Strynadka NCJ J Struct Biol. 2018 Jun 7. pii: S1047-8477(18)30140-0. doi:, 10.1016/j.jsb.2018.06.002. PMID:29886194<ref>PMID:29886194</ref>

-	~~Description~~: ~~Stage III sporulation protein AF (SpoIIIAF)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Flanagan, K.A]]	+	<div class="pdbe-citations 6dcs" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Camp~~, A.H]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Camp, A H]]
		+	[[Category: Flanagan, K A]]
		+	[[Category: Strynadka, N C.J]]
	[[Category: Zeytuni, N]]		[[Category: Zeytuni, N]]
-	[[Category: ~~Strynadka, N.C.J~~]]	+	[[Category: Protein transport]]
		+	[[Category: Secretion system]]
		+	[[Category: Sporulation]]

OCA: Protected "6dcs" [edit=sysop:move=sysop]

OCA — Wed, 16 May 2018 04:07:29 GMT

Protected "6dcs" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:07, 16 May 2018

OCA: New page: '''Unreleased structure''' The entry 6dcs is ON HOLD until Paper Publication Authors: Strynadka, N.C.J., Zeytuni, N., Camp, A.H., Flanagan, K.A. Description: Stage III sporulation prot...

OCA — Wed, 16 May 2018 04:07:28 GMT

New page: '''Unreleased structure''' The entry 6dcs is ON HOLD until Paper Publication Authors: Strynadka, N.C.J., Zeytuni, N., Camp, A.H., Flanagan, K.A. Description: Stage III sporulation prot...

New page

'''Unreleased structure'''

The entry 6dcs is ON HOLD until Paper Publication

Authors: Strynadka, N.C.J., Zeytuni, N., Camp, A.H., Flanagan, K.A.

Description: Stage III sporulation protein AF (SpoIIIAF)
[[Category: Unreleased Structures]]
[[Category: Flanagan, K.A]]
[[Category: Camp, A.H]]
[[Category: Zeytuni, N]]
[[Category: Strynadka, N.C.J]]