6ded - Revision history

OCA at 14:33, 13 March 2024

OCA — Wed, 13 Mar 2024 14:33:49 GMT

←Older revision		Revision as of 14:33, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6ded' size='340' side='right'caption='[[6ded]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='6ded' size='340' side='right'caption='[[6ded]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ded]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DED OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6DED FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ded]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DED OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DED FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~NCKIPSD~~, ~~AF3P21, SPIN90 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.204Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ded FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ded OCA], [~~http~~://pdbe.org/6ded PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ded RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ded PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ded ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ded FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ded OCA], [https://pdbe.org/6ded PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ded RCSB], [https://www.ebi.ac.uk/pdbsum/6ded PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ded ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/SPN90_HUMAN SPN90_HUMAN]] A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with KMT2A/MLL1.<ref>PMID:10648423</ref>	+	[https://www.uniprot.org/uniprot/SPN90_HUMAN SPN90_HUMAN] A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with KMT2A/MLL1.<ref>PMID:10648423</ref>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SPN90_HUMAN SPN90_HUMAN]] Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.<ref>PMID:22419821</ref>	+	[https://www.uniprot.org/uniprot/SPN90_HUMAN SPN90_HUMAN] Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.<ref>PMID:22419821</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Unlike the WASP family of Arp2/3 complex activators, WISH/DIP/SPIN90 (WDS) family proteins activate actin filament nucleation by the Arp2/3 complex without the need for a preformed actin filament. This allows WDS proteins to initiate branched actin network assembly by providing seed filaments that activate WASP-bound Arp2/3 complex. Despite their important role in actin network initiation, it is unclear how WDS proteins drive the activating steps that require both WASP and pre-existing actin filaments during WASP-mediated nucleation. Here, we show that SPIN90 folds into an armadillo repeat domain that binds a surface of Arp2/3 complex distinct from the two WASP sites, straddling a hinge point that may stimulate movement of the Arp2 subunit into the activated short-pitch conformation. SPIN90 binds a surface on Arp2/3 complex that overlaps with actin filament binding, explaining how it could stimulate the same structural rearrangements in the complex as pre-existing actin filaments. By revealing how WDS proteins activate the Arp2/3 complex, these data provide a molecular foundation to understand initiation of dendritic actin networks and regulation of Arp2/3 complex by its activators.	+
-		+
-	Structure of the nucleation-promoting factor SPIN90 bound to the actin filament nucleator Arp2/3 complex.,Luan Q, Liu SL, Helgeson LA, Nolen BJ EMBO J. 2018 Oct 15. pii: embj.2018100005. doi: 10.15252/embj.2018100005. PMID:30322896<ref>PMID:30322896</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6ded" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Luan, Q]]	+	[[Category: Luan Q]]
-	[[Category: Nolen~~, B J]]~~	+	[[Category: Nolen BJ]]
-	~~[[Category: And 2-3 armadillo repeat]]~~	+
-	~~[[Category: Endocytosis]]~~	+
-	~~[[Category: Inactive form]]~~	+
-	~~[[Category: N-terminally truncated spin90]]~~	+
-	~~[[Category: Unable to activate arp2-3 complex~~]]	+

OCA at 11:00, 1 January 2020

OCA — Wed, 01 Jan 2020 11:00:31 GMT

←Older revision		Revision as of 11:00, 1 January 2020
Line 1:		Line 1:

	==Crystal structure of the C-terminal ARM domain of Homo sapiens SPIN90 (SH3-protein interacting with Nck), residues 351-722==		==Crystal structure of the C-terminal ARM domain of Homo sapiens SPIN90 (SH3-protein interacting with Nck), residues 351-722==
-	<StructureSection load='6ded' size='340' side='right' caption='[[6ded]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='6ded' size='340' side='right'caption='[[6ded]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ded]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DED OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DED FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ded]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DED OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DED FirstGlance]. <br>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Luan, Q]]		[[Category: Luan, Q]]
	[[Category: Nolen, B J]]		[[Category: Nolen, B J]]

OCA at 07:14, 31 October 2018

OCA — Wed, 31 Oct 2018 07:14:04 GMT

←Older revision		Revision as of 07:14, 31 October 2018
Line 3:		Line 3:
	<StructureSection load='6ded' size='340' side='right' caption='[[6ded]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='6ded' size='340' side='right' caption='[[6ded]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ded]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DED OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DED FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ded]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DED OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DED FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ded FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ded OCA], [http://pdbe.org/6ded PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ded RCSB], [http://www.ebi.ac.uk/pdbsum/6ded PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ded ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCKIPSD, AF3P21, SPIN90 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ded FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ded OCA], [http://pdbe.org/6ded PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ded RCSB], [http://www.ebi.ac.uk/pdbsum/6ded PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ded ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 10:		Line 11:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SPN90_HUMAN SPN90_HUMAN]] Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.<ref>PMID:22419821</ref>		[[http://www.uniprot.org/uniprot/SPN90_HUMAN SPN90_HUMAN]] Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.<ref>PMID:22419821</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Unlike the WASP family of Arp2/3 complex activators, WISH/DIP/SPIN90 (WDS) family proteins activate actin filament nucleation by the Arp2/3 complex without the need for a preformed actin filament. This allows WDS proteins to initiate branched actin network assembly by providing seed filaments that activate WASP-bound Arp2/3 complex. Despite their important role in actin network initiation, it is unclear how WDS proteins drive the activating steps that require both WASP and pre-existing actin filaments during WASP-mediated nucleation. Here, we show that SPIN90 folds into an armadillo repeat domain that binds a surface of Arp2/3 complex distinct from the two WASP sites, straddling a hinge point that may stimulate movement of the Arp2 subunit into the activated short-pitch conformation. SPIN90 binds a surface on Arp2/3 complex that overlaps with actin filament binding, explaining how it could stimulate the same structural rearrangements in the complex as pre-existing actin filaments. By revealing how WDS proteins activate the Arp2/3 complex, these data provide a molecular foundation to understand initiation of dendritic actin networks and regulation of Arp2/3 complex by its activators.
		+
		+	Structure of the nucleation-promoting factor SPIN90 bound to the actin filament nucleator Arp2/3 complex.,Luan Q, Liu SL, Helgeson LA, Nolen BJ EMBO J. 2018 Oct 15. pii: embj.2018100005. doi: 10.15252/embj.2018100005. PMID:30322896<ref>PMID:30322896</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ded" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Luan, Q]]		[[Category: Luan, Q]]
	[[Category: Nolen, B J]]		[[Category: Nolen, B J]]

OCA at 05:53, 24 October 2018

OCA — Wed, 24 Oct 2018 05:53:26 GMT

←Older revision		Revision as of 05:53, 24 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6ded is ON HOLD until Paper Publication~~	+	==Crystal structure of the C-terminal ARM domain of Homo sapiens SPIN90 (SH3-protein interacting with Nck), residues 351-722==
-		+	<StructureSection load='6ded' size='340' side='right' caption='[[6ded]], [[Resolution\|resolution]] 2.20Å' scene=''>
-	~~Authors: Nolen, B.J., Luan, Q.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ded]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DED OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DED FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the C-terminal ARM domain of Homo sapiens SPIN90 (SH3-protein interacting with Nck), residues 351-722	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ded FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ded OCA], [http://pdbe.org/6ded PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ded RCSB], [http://www.ebi.ac.uk/pdbsum/6ded PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ded ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Nolen~~, B.J]]	+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/SPN90_HUMAN SPN90_HUMAN]] A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with KMT2A/MLL1.<ref>PMID:10648423</ref>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPN90_HUMAN SPN90_HUMAN]] Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.<ref>PMID:22419821</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Luan, Q]]		[[Category: Luan, Q]]
		+	[[Category: Nolen, B J]]
		+	[[Category: And 2-3 armadillo repeat]]
		+	[[Category: Endocytosis]]
		+	[[Category: Inactive form]]
		+	[[Category: N-terminally truncated spin90]]
		+	[[Category: Unable to activate arp2-3 complex]]

OCA at 05:35, 14 June 2018

OCA — Thu, 14 Jun 2018 05:35:06 GMT

←Older revision		Revision as of 05:35, 14 June 2018
Line 5:		Line 5:
	Authors: Nolen, B.J., Luan, Q.		Authors: Nolen, B.J., Luan, Q.

-	Description: Crystal structure of Homo sapiens SPIN90 ~~residue~~ 351-722	+	Description: Crystal structure of the C-terminal ARM domain of Homo sapiens SPIN90 (SH3-protein interacting with Nck), residues 351-722
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Nolen, B.J]]		[[Category: Nolen, B.J]]
	[[Category: Luan, Q]]		[[Category: Luan, Q]]

OCA at 04:04, 30 May 2018

OCA — Wed, 30 May 2018 04:04:09 GMT

←Older revision		Revision as of 04:04, 30 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ded is ON HOLD	+	The entry 6ded is ON HOLD until Paper Publication

	Authors: Nolen, B.J., Luan, Q.		Authors: Nolen, B.J., Luan, Q.
Line 7:		Line 7:
	Description: Crystal structure of Homo sapiens SPIN90 residue 351-722		Description: Crystal structure of Homo sapiens SPIN90 residue 351-722
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Luan, Q]]
	[[Category: Nolen, B.J]]		[[Category: Nolen, B.J]]
		+	[[Category: Luan, Q]]

OCA: Protected "6ded" [edit=sysop:move=sysop]

OCA — Wed, 23 May 2018 06:00:35 GMT

Protected "6ded" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:00, 23 May 2018

OCA: New page: '''Unreleased structure''' The entry 6ded is ON HOLD Authors: Nolen, B.J., Luan, Q. Description: Crystal structure of Homo sapiens SPIN90 residue 351-722 [[Category: Unreleased Structu...

OCA — Wed, 23 May 2018 06:00:34 GMT

New page: '''Unreleased structure''' The entry 6ded is ON HOLD Authors: Nolen, B.J., Luan, Q. Description: Crystal structure of Homo sapiens SPIN90 residue 351-722 [[Category: Unreleased Structu...

New page

'''Unreleased structure'''

The entry 6ded is ON HOLD

Authors: Nolen, B.J., Luan, Q.

Description: Crystal structure of Homo sapiens SPIN90 residue 351-722
[[Category: Unreleased Structures]]
[[Category: Luan, Q]]
[[Category: Nolen, B.J]]