6dgv - Revision history

OCA at 06:05, 11 October 2023

OCA — Wed, 11 Oct 2023 06:05:59 GMT

←Older revision		Revision as of 06:05, 11 October 2023
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	<StructureSection load='6dgv' size='340' side='right'caption='[[6dgv]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='6dgv' size='340' side='right'caption='[[6dgv]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dgv]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus~~ fluorescens ~~liquefaciens" flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6DGV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dgv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DGV FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non~~-~~Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6dgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgv OCA], [~~http~~://pdbe.org/6dgv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6dgv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6dgv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgv ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgv OCA], [https://pdbe.org/6dgv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dgv RCSB], [https://www.ebi.ac.uk/pdbsum/6dgv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1B3D787_PSEFL A0A1B3D787_PSEFL]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus fluorescens liquefaciens flugge 1886]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Looger, L L~~]]	+	[[Category: Pseudomonas fluorescens]]
-	[[Category: ~~Marvin, J S~~]]	+	[[Category: Looger LL]]
-	[[Category: ~~Circularly permuted gfp]]~~	+	[[Category: Marvin JS]]
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Fluorescent sensor]]~~	+
-	~~[[Category: Gaba binding protein]]~~	+
-	~~[[Category: Periplasmic binding protein]]~~	+
-	~~[[Category: Superfolder~~]]	+

OCA at 06:45, 31 July 2019

OCA — Wed, 31 Jul 2019 06:45:17 GMT

←Older revision		Revision as of 06:45, 31 July 2019
Line 3:		Line 3:
	<StructureSection load='6dgv' size='340' side='right'caption='[[6dgv]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='6dgv' size='340' side='right'caption='[[6dgv]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dgv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DGV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dgv]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_liquefaciens"_flugge_1886 "bacillus fluorescens liquefaciens" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DGV FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgv OCA], [http://pdbe.org/6dgv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dgv RCSB], [http://www.ebi.ac.uk/pdbsum/6dgv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgv OCA], [http://pdbe.org/6dgv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dgv RCSB], [http://www.ebi.ac.uk/pdbsum/6dgv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgv ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Current techniques for monitoring GABA (gamma-aminobutyric acid), the primary inhibitory neurotransmitter in vertebrates, cannot follow transients in intact neural circuits. To develop a GABA sensor, we applied the design principles used to create the fluorescent glutamate receptor iGluSnFR. We used a protein derived from a previously unsequenced Pseudomonas fluorescens strain and performed structure-guided mutagenesis and library screening to obtain intensity-based GABA sensing fluorescence reporter (iGABASnFR) variants. iGABASnFR is genetically encoded, detects GABA release evoked by electric stimulation of afferent fibers in acute brain slices and produces readily detectable fluorescence increases in vivo in mice and zebrafish. We applied iGABASnFR to track mitochondrial GABA content and its modulation by an anticonvulsant, swimming-evoked, GABA-mediated transmission in zebrafish cerebellum, GABA release events during interictal spikes and seizures in awake mice, and found that GABA-mediated tone decreases during isoflurane anesthesia.
		+
		+	A genetically encoded fluorescent sensor for in vivo imaging of GABA.,Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA, Ahrens MB, Kullmann DM, Looger LL Nat Methods. 2019 Jul 15. pii: 10.1038/s41592-019-0471-2. doi:, 10.1038/s41592-019-0471-2. PMID:31308547<ref>PMID:31308547</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6dgv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus fluorescens liquefaciens flugge 1886]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Looger, L L]]		[[Category: Looger, L L]]

OCA at 06:53, 3 April 2019

OCA — Wed, 03 Apr 2019 06:53:06 GMT

←Older revision		Revision as of 06:53, 3 April 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6dgv is ~~ON HOLD~~	+	==iGABASnFR Fluorescent GABA Sensor precursor==
-		+	<StructureSection load='6dgv' size='340' side='right'caption='[[6dgv]], [[Resolution\|resolution]] 2.80Å' scene=''>
-	~~Authors~~: ~~Marvin~~, J.S., ~~Looger~~, L.L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6dgv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DGV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DGV FirstGlance]. <br>
-	~~Description: iGABASnFR Fluorescent GABA Sensor precursor~~	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dgv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dgv OCA], [http://pdbe.org/6dgv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dgv RCSB], [http://www.ebi.ac.uk/pdbsum/6dgv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dgv ProSAT]</span></td></tr>
-	[[Category: Looger, L.L]]	+	</table>
-	[[Category: Marvin, J.S]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Looger, L L]]
		+	[[Category: Marvin, J S]]
		+	[[Category: Circularly permuted gfp]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Fluorescent sensor]]
		+	[[Category: Gaba binding protein]]
		+	[[Category: Periplasmic binding protein]]
		+	[[Category: Superfolder]]

OCA at 05:58, 30 January 2019

OCA — Wed, 30 Jan 2019 05:58:21 GMT

←Older revision		Revision as of 05:58, 30 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6dgv is ON HOLD ~~until sometime in the future~~	+	The entry 6dgv is ON HOLD

	Authors: Marvin, J.S., Looger, L.L.		Authors: Marvin, J.S., Looger, L.L.
Line 7:		Line 7:
	Description: iGABASnFR Fluorescent GABA Sensor precursor		Description: iGABASnFR Fluorescent GABA Sensor precursor
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Marvin, J.S]]
	[[Category: Looger, L.L]]		[[Category: Looger, L.L]]
		+	[[Category: Marvin, J.S]]

OCA at 10:18, 16 January 2019

OCA — Wed, 16 Jan 2019 10:18:17 GMT

←Older revision		Revision as of 10:18, 16 January 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6dgv is ON HOLD	+	The entry 6dgv is ON HOLD until sometime in the future

	Authors: Marvin, J.S., Looger, L.L.		Authors: Marvin, J.S., Looger, L.L.
Line 7:		Line 7:
	Description: iGABASnFR Fluorescent GABA Sensor precursor		Description: iGABASnFR Fluorescent GABA Sensor precursor
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Looger, L.L]]
	[[Category: Marvin, J.S]]		[[Category: Marvin, J.S]]
		+	[[Category: Looger, L.L]]

OCA at 06:22, 19 December 2018

OCA — Wed, 19 Dec 2018 06:22:08 GMT

←Older revision		Revision as of 06:22, 19 December 2018
Line 5:		Line 5:
	Authors: Marvin, J.S., Looger, L.L.		Authors: Marvin, J.S., Looger, L.L.

-	Description: iGABASnFR Fluorescent GABA Sensor	+	Description: iGABASnFR Fluorescent GABA Sensor precursor
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Looger, L.L]]		[[Category: Looger, L.L]]
	[[Category: Marvin, J.S]]		[[Category: Marvin, J.S]]

OCA: Protected "6dgv" [edit=sysop:move=sysop]

OCA — Wed, 30 May 2018 04:05:52 GMT

Protected "6dgv" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:05, 30 May 2018

OCA: New page: '''Unreleased structure''' The entry 6dgv is ON HOLD Authors: Marvin, J.S., Looger, L.L. Description: iGABASnFR Fluorescent GABA Sensor Category: Unreleased Structures [[Category: ...

OCA — Wed, 30 May 2018 04:05:50 GMT

New page: '''Unreleased structure''' The entry 6dgv is ON HOLD Authors: Marvin, J.S., Looger, L.L. Description: iGABASnFR Fluorescent GABA Sensor Category: Unreleased Structures [[Category: ...

New page

'''Unreleased structure'''

The entry 6dgv is ON HOLD

Authors: Marvin, J.S., Looger, L.L.

Description: iGABASnFR Fluorescent GABA Sensor
[[Category: Unreleased Structures]]
[[Category: Looger, L.L]]
[[Category: Marvin, J.S]]