6dhx - Revision history

OCA at 10:09, 18 December 2019

OCA — Wed, 18 Dec 2019 10:09:28 GMT

←Older revision		Revision as of 10:09, 18 December 2019
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	==Structure of TipC2 from Streptococcus intermedius B196==		==Structure of TipC2 from Streptococcus intermedius B196==
-	<StructureSection load='6dhx' size='340' side='right' caption='[[6dhx]], [[Resolution\|resolution]] 1.75Å' scene=''>	+	<StructureSection load='6dhx' size='340' side='right'caption='[[6dhx]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6dhx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_intermedius_b196 Streptococcus intermedius b196]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DHX FirstGlance]. <br>		<table><tr><td colspan='2'>[[6dhx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_intermedius_b196 Streptococcus intermedius b196]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DHX FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Streptococcus intermedius b196]]		[[Category: Streptococcus intermedius b196]]
	[[Category: Bryant, D]]		[[Category: Bryant, D]]

OCA at 06:48, 24 October 2018

OCA — Wed, 24 Oct 2018 06:48:27 GMT

←Older revision		Revision as of 06:48, 24 October 2018
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	<StructureSection load='6dhx' size='340' side='right' caption='[[6dhx]], [[Resolution\|resolution]] 1.75Å' scene=''>		<StructureSection load='6dhx' size='340' side='right' caption='[[6dhx]], [[Resolution\|resolution]] 1.75Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6dhx]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DHX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6dhx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_intermedius_b196 Streptococcus intermedius b196]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DHX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SIR_1486 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=862967 Streptococcus intermedius B196])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dhx OCA], [http://pdbe.org/6dhx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dhx RCSB], [http://www.ebi.ac.uk/pdbsum/6dhx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dhx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dhx OCA], [http://pdbe.org/6dhx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dhx RCSB], [http://www.ebi.ac.uk/pdbsum/6dhx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dhx ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Streptococcus intermedius b196]]
	[[Category: Bryant, D]]		[[Category: Bryant, D]]
	[[Category: Klein, T A]]		[[Category: Klein, T A]]

OCA at 19:49, 19 September 2018

OCA — Wed, 19 Sep 2018 19:49:48 GMT

←Older revision		Revision as of 19:49, 19 September 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6dhx is ~~ON HOLD until Paper~~ Publication	+	==Structure of TipC2 from Streptococcus intermedius B196==
		+	<StructureSection load='6dhx' size='340' side='right' caption='[[6dhx]], [[Resolution\|resolution]] 1.75Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6dhx]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DHX FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dhx OCA], [http://pdbe.org/6dhx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dhx RCSB], [http://www.ebi.ac.uk/pdbsum/6dhx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dhx ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gram-positive bacteria deploy the type VII secretion system (T7SS) to facilitate interactions between eukaryotic and prokaryotic cells. In recent work, we identified the TelC protein from Streptococcus intermedius as a T7SS-exported lipid II phosphatase that mediates interbacterial competition. TelC exerts toxicity in the inner wall zone of Gram-positive bacteria; however, intercellular intoxication of sister cells does not occur because they express the TipC immunity protein. In the present study, we sought to characterize the molecular basis of self-protection by TipC. Using sub-cellular localization and protease protection assays, we show that TipC is a membrane protein with an N-terminal transmembrane segment and a C-terminal TelC-inhibitory domain that protrudes into the inner wall zone. The 1.9A X-ray crystal structure of a non-protective TipC paralogue reveals that the soluble domain of TipC proteins adopts a crescent-shaped fold comprised of three alpha-helices and a seven-stranded beta-sheet. Subsequent homology-guided mutagenesis demonstrates that a concave surface formed by the predicted beta-sheet of TipC is required for both its interaction with TelC and its TelC-inhibitory activity. S. intermedius cells lacking the tipC gene are susceptible to growth inhibition by TelC delivered between cells; however, we find that the growth of this strain is unaffected by endogenous or overexpressed TelC even though the toxin accumulates in culture supernatants. Together, these data indicate that the TelC-inhibitory activity of TipC is only required for intercellularly-transferred TelC and that the T7SS apparatus transports TelC across the cell envelope in a single step, bypassing the cellular compartment in which it exerts toxicity en route.

-	~~Authors~~:	+	Molecular Basis for Immunity Protein Recognition of a Type VII Secretion System Exported Antibacterial Toxin.,Klein TA, Pazos M, Surette MG, Vollmer W, Whitney JC J Mol Biol. 2018 Sep 5. pii: S0022-2836(18)30594-1. doi:, 10.1016/j.jmb.2018.08.027. PMID:30194969<ref>PMID:30194969</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6dhx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bryant, D]]
		+	[[Category: Klein, T A]]
		+	[[Category: Whitney, J C]]
		+	[[Category: Antitoxin]]
		+	[[Category: Type vii secretion immunity protein]]

OCA at 05:20, 18 July 2018

OCA — Wed, 18 Jul 2018 05:20:01 GMT

←Older revision		Revision as of 05:20, 18 July 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6dhx is ON HOLD	+	The entry 6dhx is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6dhx" [edit=sysop:move=sysop]

OCA — Wed, 30 May 2018 04:08:59 GMT

Protected "6dhx" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:08, 30 May 2018

OCA: New page: '''Unreleased structure''' The entry 6dhx is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 30 May 2018 04:08:58 GMT

New page: '''Unreleased structure''' The entry 6dhx is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6dhx is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]