6e0g - Revision history

OCA at 14:38, 13 March 2024

OCA — Wed, 13 Mar 2024 14:38:08 GMT

←Older revision		Revision as of 14:38, 13 March 2024
Line 3:		Line 3:
	<SX load='6e0g' size='340' side='right' viewer='molstar' caption='[[6e0g]], [[Resolution\|resolution]] 2.90Å' scene=''>		<SX load='6e0g' size='340' side='right' viewer='molstar' caption='[[6e0g]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6e0g]] is a 10 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Leiin Leiin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6E0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6e0g]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_infantum Leishmania infantum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E0G FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6e0f\|6e0f]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">mTXNPx, LINJ_23_0050 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=5671 LEIIN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0g OCA], [https://pdbe.org/6e0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e0g RCSB], [https://www.ebi.ac.uk/pdbsum/6e0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0g ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6e0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0g OCA], [~~http~~://pdbe.org/6e0g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6e0g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6e0g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0g ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A6L0XFC6_LEIIN A0A6L0XFC6_LEIIN] Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively.[PIRNR:PIRNR000239]
-	~~Many 2-Cys-peroxiredoxins (2-Cys-Prxs) are dual-function proteins, either acting as peroxidases under non-stress conditions or as chaperones during stress~~. ~~The mechanism by which 2-Cys-Prxs switch functions remains to be defined~~. ~~Our work focuses on Leishmania infantum mitochondrial 2~~-~~Cys-Prx, whose reduced, decameric subpopulation adopts chaperone function during heat shock, an activity~~ that ~~facilitates~~ the ~~transition from insects to warm-blooded host environments. Here, we have solved the cryo-EM structure~~ of ~~mTXNPx in complex with a thermally unfolded client protein,~~ and ~~revealed that the flexible N-termini of mTXNPx form a well-resolved central belt that contacts~~ and ~~encapsulates the unstructured client protein in the center of the decamer ring. In vivo and in vitro cross-linking studies provide further support for these interactions~~, ~~and demonstrate that mTXNPx decamers undergo temperature-dependent structural rearrangements specifically at the dimer-dimer interfaces~~. ~~These structural changes appear crucial for exposing chaperone-client binding sites that are buried in the peroxidase-active protein.~~	+
-		+
-	Chaperone activation and client binding of a 2-cysteine peroxiredoxin.,Teixeira F, Tse E, Castro H, Makepeace KAT, Meinen BA, Borchers CH, Poole LB, Bardwell JC, Tomas AM, Southworth DR, Jakob U Nat Commun. 2019 Feb 8;10(1):~~659. doi: 10.1038/s41467-019-08565-8. PMID:30737390<ref>PMID:30737390</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6e0g" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Peroxiredoxin 3D structures\|Peroxiredoxin 3D structures]]		*[[Peroxiredoxin 3D structures\|Peroxiredoxin 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Leiin~~]]	+	[[Category: Leishmania infantum]]
-	[[Category: Borchers~~, C H~~]]	+	[[Category: Borchers CH]]
-	[[Category: Castro, H]]	+	[[Category: Castro H]]
-	[[Category: Jakob, U]]	+	[[Category: Jakob U]]
-	[[Category: Makepeace~~, K A.T~~]]	+	[[Category: Makepeace KAT]]
-	[[Category: Poole~~, L B~~]]	+	[[Category: Poole LB]]
-	[[Category: Southworth~~, D R~~]]	+	[[Category: Southworth DR]]
-	[[Category: Teixeira, F]]	+	[[Category: Teixeira F]]
-	[[Category: Tomas~~, A M~~]]	+	[[Category: Tomas AM]]
-	[[Category: Tse, E]]	+	[[Category: Tse E]]
-	~~[[Category: Chaperone]]~~	+
-	~~[[Category: Client-binding]]~~	+
-	~~[[Category: Heat-shock]]~~	+
-	~~[[Category: Holdase]]~~	+
-	~~[[Category: Unfolding~~]]	+

OCA at 00:16, 11 April 2020

OCA — Sat, 11 Apr 2020 00:16:12 GMT

←Older revision		Revision as of 00:16, 11 April 2020
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	<SX load='6e0g' size='340' side='right' viewer='molstar' caption='[[6e0g]], [[Resolution\|resolution]] 2.90Å' scene=''>		<SX load='6e0g' size='340' side='right' viewer='molstar' caption='[[6e0g]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6e0g]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiin Leiin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0G OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6E0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6e0g]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiin Leiin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6E0G FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6e0f\|6e0f]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6e0f\|6e0f]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mTXNPx, LINJ_23_0050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5671 LEIIN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mTXNPx, LINJ_23_0050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5671 LEIIN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6e0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0g OCA], [http://pdbe.org/6e0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e0g RCSB], [http://www.ebi.ac.uk/pdbsum/6e0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0g ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6e0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0g OCA], [http://pdbe.org/6e0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e0g RCSB], [http://www.ebi.ac.uk/pdbsum/6e0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0g ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 20:59, 6 March 2020

OCA — Fri, 06 Mar 2020 20:59:30 GMT

←Older revision		Revision as of 20:59, 6 March 2020
Line 1:		Line 1:

	==Mitochondrial peroxiredoxin from Leishmania infantum after heat stress without unfolding client protein==		==Mitochondrial peroxiredoxin from Leishmania infantum after heat stress without unfolding client protein==
-	<~~StructureSection~~ load='6e0g' size='340' side='right'caption='[[6e0g]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<SX load='6e0g' size='340' side='right' viewer='molstar' caption='[[6e0g]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6e0g]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiin Leiin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E0G FirstGlance]. <br>		<table><tr><td colspan='2'>[[6e0g]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiin Leiin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E0G FirstGlance]. <br>
Line 23:		Line 23:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Leiin]]		[[Category: Leiin]]

OCA at 10:19, 18 December 2019

OCA — Wed, 18 Dec 2019 10:19:45 GMT

←Older revision		Revision as of 10:19, 18 December 2019
Line 1:		Line 1:

	==Mitochondrial peroxiredoxin from Leishmania infantum after heat stress without unfolding client protein==		==Mitochondrial peroxiredoxin from Leishmania infantum after heat stress without unfolding client protein==
-	<StructureSection load='6e0g' size='340' side='right' caption='[[6e0g]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='6e0g' size='340' side='right'caption='[[6e0g]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6e0g]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6e0g]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Leiin Leiin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E0G FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6e0f\|6e0f]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6e0f\|6e0f]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mTXNPx, LINJ_23_0050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5671 LEIIN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0g OCA], [http://pdbe.org/6e0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e0g RCSB], [http://www.ebi.ac.uk/pdbsum/6e0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0g ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0g OCA], [http://pdbe.org/6e0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e0g RCSB], [http://www.ebi.ac.uk/pdbsum/6e0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0g ProSAT]</span></td></tr>
	</table>		</table>
Line 16:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 6e0g" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6e0g" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Peroxiredoxin 3D structures\|Peroxiredoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Leiin]]
	[[Category: Borchers, C H]]		[[Category: Borchers, C H]]
	[[Category: Castro, H]]		[[Category: Castro, H]]

OCA at 06:39, 21 February 2019

OCA — Thu, 21 Feb 2019 06:39:19 GMT

←Older revision		Revision as of 06:39, 21 February 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6e0g is ~~ON HOLD until Paper~~ Publication	+	==Mitochondrial peroxiredoxin from Leishmania infantum after heat stress without unfolding client protein==
		+	<StructureSection load='6e0g' size='340' side='right' caption='[[6e0g]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6e0g]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E0G FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6e0f\|6e0f]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e0g OCA], [http://pdbe.org/6e0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e0g RCSB], [http://www.ebi.ac.uk/pdbsum/6e0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e0g ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many 2-Cys-peroxiredoxins (2-Cys-Prxs) are dual-function proteins, either acting as peroxidases under non-stress conditions or as chaperones during stress. The mechanism by which 2-Cys-Prxs switch functions remains to be defined. Our work focuses on Leishmania infantum mitochondrial 2-Cys-Prx, whose reduced, decameric subpopulation adopts chaperone function during heat shock, an activity that facilitates the transition from insects to warm-blooded host environments. Here, we have solved the cryo-EM structure of mTXNPx in complex with a thermally unfolded client protein, and revealed that the flexible N-termini of mTXNPx form a well-resolved central belt that contacts and encapsulates the unstructured client protein in the center of the decamer ring. In vivo and in vitro cross-linking studies provide further support for these interactions, and demonstrate that mTXNPx decamers undergo temperature-dependent structural rearrangements specifically at the dimer-dimer interfaces. These structural changes appear crucial for exposing chaperone-client binding sites that are buried in the peroxidase-active protein.

-	~~Authors~~:	+	Chaperone activation and client binding of a 2-cysteine peroxiredoxin.,Teixeira F, Tse E, Castro H, Makepeace KAT, Meinen BA, Borchers CH, Poole LB, Bardwell JC, Tomas AM, Southworth DR, Jakob U Nat Commun. 2019 Feb 8;10(1):659. doi: 10.1038/s41467-019-08565-8. PMID:30737390<ref>PMID:30737390</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6e0g" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Borchers, C H]]
		+	[[Category: Castro, H]]
		+	[[Category: Jakob, U]]
		+	[[Category: Makepeace, K A.T]]
		+	[[Category: Poole, L B]]
		+	[[Category: Southworth, D R]]
		+	[[Category: Teixeira, F]]
		+	[[Category: Tomas, A M]]
		+	[[Category: Tse, E]]
		+	[[Category: Chaperone]]
		+	[[Category: Client-binding]]
		+	[[Category: Heat-shock]]
		+	[[Category: Holdase]]
		+	[[Category: Unfolding]]

OCA at 17:01, 1 August 2018

OCA — Wed, 01 Aug 2018 17:01:05 GMT

←Older revision		Revision as of 17:01, 1 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6e0g is ON HOLD	+	The entry 6e0g is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6e0g" [edit=sysop:move=sysop]

OCA — Wed, 18 Jul 2018 05:32:17 GMT

Protected "6e0g" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 18 July 2018

OCA: New page: '''Unreleased structure''' The entry 6e0g is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 18 Jul 2018 05:32:15 GMT

New page: '''Unreleased structure''' The entry 6e0g is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6e0g is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]