6e1j - Revision history

OCA at 06:17, 11 October 2023

OCA — Wed, 11 Oct 2023 06:17:21 GMT

←Older revision		Revision as of 06:17, 11 October 2023
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	<StructureSection load='6e1j' size='340' side='right'caption='[[6e1j]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6e1j' size='340' side='right'caption='[[6e1j]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6e1j]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Braju Braju~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E1J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6E1J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6e1j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brassica_juncea Brassica juncea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E1J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=KMT:4-(METHYLSULFANYL)-2-OXOBUTANOIC+ACID'>KMT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.096Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gsl-elong-A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3707 BRAJU])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=KMT:4-(METHYLSULFANYL)-2-OXOBUTANOIC+ACID'>KMT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6e1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e1j OCA], [~~http~~://pdbe.org/6e1j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6e1j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6e1j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6e1j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e1j OCA], [https://pdbe.org/6e1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e1j RCSB], [https://www.ebi.ac.uk/pdbsum/6e1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e1j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C5J4P1_BRAJU C5J4P1_BRAJU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6e1j" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6e1j" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[2-isopropylmalate synthase 3D structures\|2-isopropylmalate synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Braju~~]]	+	[[Category: Brassica juncea]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Jez~~, J M~~]]	+	[[Category: Jez JM]]
-	[[Category: Lee~~, S G]]~~	+	[[Category: Lee SG]]
-	~~[[Category: Enzyme]]~~	+
-	~~[[Category: Methylthioalkylmalate synthase]]~~	+
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: X-ray crystal structure~~]]	+

OCA at 08:32, 24 July 2019

OCA — Wed, 24 Jul 2019 08:32:59 GMT

←Older revision		Revision as of 08:32, 24 July 2019
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	<StructureSection load='6e1j' size='340' side='right'caption='[[6e1j]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6e1j' size='340' side='right'caption='[[6e1j]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6e1j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E1J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6e1j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Braju Braju]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E1J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=KMT:4-(METHYLSULFANYL)-2-OXOBUTANOIC+ACID'>KMT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=KMT:4-(METHYLSULFANYL)-2-OXOBUTANOIC+ACID'>KMT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">gsl-elong-A1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3707 BRAJU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e1j OCA], [http://pdbe.org/6e1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e1j RCSB], [http://www.ebi.ac.uk/pdbsum/6e1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e1j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e1j OCA], [http://pdbe.org/6e1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e1j RCSB], [http://www.ebi.ac.uk/pdbsum/6e1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e1j ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Braju]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jez, J M]]		[[Category: Jez, J M]]

OCA at 11:16, 10 May 2019

OCA — Fri, 10 May 2019 11:16:05 GMT

←Older revision		Revision as of 11:16, 10 May 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6e1j is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of Methylthioalkylmalate Synthase (BjuMAM1.1) from Brassica juncea==
		+	<StructureSection load='6e1j' size='340' side='right'caption='[[6e1j]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6e1j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E1J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E1J FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=KMT:4-(METHYLSULFANYL)-2-OXOBUTANOIC+ACID'>KMT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e1j OCA], [http://pdbe.org/6e1j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e1j RCSB], [http://www.ebi.ac.uk/pdbsum/6e1j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e1j ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Methylthioalkylmalate synthase catalyzes the committed step in the side-chain elongation of methionine-derived aliphatic glucosinolates and likely evolved from the isopropylmalate synthases of leucine biosynthesis. The globally cultivated Brassica species possess diverse aliphatic glucosinolates important for plant defense and animal nutrition; however, the molecular basis for the evolution of methylthioalkylmalate synthase and its generation of natural product diversity in Brassica is poorly understood. Here we show that Brassica genomes encode multiple methylthioalkylmalate synthase that have differences in expression profiles and 2-oxo substrate preference that account for diversity of aliphatic glucosinolates across Brassica accessions. The 2.1 A resolution x-ray crystal structure of B. juncea methylthioalkylmalate synthase identifies key active site residues responsible for controlling specificity for different 2-oxo substrates and the determinants of side-chain length in aliphatic glucosinolates. Overall, these results provide the evolutionary and biochemical foundation for diversification of glucosinolates profiles across globally-cultivated Brassica species, which could be used with ongoing breeding strategies towards manipulation of beneficial glucosinolates compounds for animal health and plant protection.

-	~~Authors:~~ Lee, ~~S.G.~~, ~~Jez~~, J.M.	+	Molecular Basis of the Evolution of Methylthioalkylmalate Synthase and Diversity of Methionine-Derived Glucosinolates.,Kumar R, Lee SG, Augustine R, Reichelt M, Vassao DG, Palavalli MH, Allen A, Gershenzon J, Jez JM, Bisht NC Plant Cell. 2019 Apr 25. pii: tpc.19.00046. doi: 10.1105/tpc.19.00046. PMID:31023839<ref>PMID:31023839</ref>

-	~~Description: Crystal Structure~~ of ~~Methylthioalkylmalate Synthase (BjuMAM1~~.~~1) from Brassica juncea~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Lee, S.G]]	+	<div class="pdbe-citations 6e1j" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Jez, J.M~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Jez, J M]]
		+	[[Category: Lee, S G]]
		+	[[Category: Enzyme]]
		+	[[Category: Methylthioalkylmalate synthase]]
		+	[[Category: Plant protein]]
		+	[[Category: X-ray crystal structure]]

OCA at 08:40, 5 September 2018

OCA — Wed, 05 Sep 2018 08:40:49 GMT

←Older revision		Revision as of 08:40, 5 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6e1j is ON HOLD	+	The entry 6e1j is ON HOLD until Paper Publication

	Authors: Lee, S.G., Jez, J.M.		Authors: Lee, S.G., Jez, J.M.

OCA: Protected "6e1j" [edit=sysop:move=sysop]

OCA — Wed, 18 Jul 2018 05:39:42 GMT

Protected "6e1j" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:39, 18 July 2018

OCA: New page: '''Unreleased structure''' The entry 6e1j is ON HOLD Authors: Lee, S.G., Jez, J.M. Description: Crystal Structure of Methylthioalkylmalate Synthase (BjuMAM1.1) from Brassica juncea [[C...

OCA — Wed, 18 Jul 2018 05:39:40 GMT

New page: '''Unreleased structure''' The entry 6e1j is ON HOLD Authors: Lee, S.G., Jez, J.M. Description: Crystal Structure of Methylthioalkylmalate Synthase (BjuMAM1.1) from Brassica juncea [[C...

New page

'''Unreleased structure'''

The entry 6e1j is ON HOLD

Authors: Lee, S.G., Jez, J.M.

Description: Crystal Structure of Methylthioalkylmalate Synthase (BjuMAM1.1) from Brassica juncea
[[Category: Unreleased Structures]]
[[Category: Lee, S.G]]
[[Category: Jez, J.M]]