6e6s - Revision history

OCA at 06:20, 11 October 2023

OCA — Wed, 11 Oct 2023 06:20:06 GMT

←Older revision		Revision as of 06:20, 11 October 2023
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	<StructureSection load='6e6s' size='340' side='right'caption='[[6e6s]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='6e6s' size='340' side='right'caption='[[6e6s]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6e6s]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E6S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6E6S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6e6s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E6S FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">bfd, AO896_12000, AO964_01120, AOY09_04877, C8257_08100, CAZ03_03910, CAZ10_19915, CGU42_05470, CSB93_2785, PAERUG_E15_London_28_01_14_09195, PAMH19_1562, RW109_RW109_02290 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~287~~ "~~Bacillus aeruginosus~~" (~~Schroeter 1872) Trevisan 1885]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6e6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e6s OCA], [~~http~~://pdbe.org/6e6s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6e6s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6e6s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6e6s ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e6s OCA], [https://pdbe.org/6e6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e6s RCSB], [https://www.ebi.ac.uk/pdbsum/6e6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e6s ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9HY80_PSEAE Q9HY80_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Battaile~~, K P~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: Lovell, S]]	+	[[Category: Battaile KP]]
-	[[Category: Rivera, M]]	+	[[Category: Lovell S]]
-	[[Category: Wang, Y]]	+	[[Category: Rivera M]]
-	[[Category: Wijerathne, H]]	+	[[Category: Wang Y]]
-	[[Category: Yao, H]]	+	[[Category: Wijerathne H]]
-	~~[[Category: Anion binding site]]~~	+	[[Category: Yao H]]
-	~~[[Category: Bfd]]~~	+
-	~~[[Category: Electron transport]]~~	+
-	~~[[Category: Iron mobilization~~]]	+

OCA at 10:26, 18 December 2019

OCA — Wed, 18 Dec 2019 10:26:55 GMT

←Older revision		Revision as of 10:26, 18 December 2019
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	==1.45 A resolution structure of the C-terminally truncated [2Fe-2S] ferredoxin (Bfd) R26E/K46Y mutant from Pseudomonas aeruginosa==		==1.45 A resolution structure of the C-terminally truncated [2Fe-2S] ferredoxin (Bfd) R26E/K46Y mutant from Pseudomonas aeruginosa==
-	<StructureSection load='6e6s' size='340' side='right' caption='[[6e6s]], [[Resolution\|resolution]] 1.45Å' scene=''>	+	<StructureSection load='6e6s' size='340' side='right'caption='[[6e6s]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6e6s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E6S FirstGlance]. <br>		<table><tr><td colspan='2'>[[6e6s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E6S FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 6e6s" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6e6s" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ferredoxin 3D structures\|Ferredoxin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Battaile, K P]]		[[Category: Battaile, K P]]
	[[Category: Lovell, S]]		[[Category: Lovell, S]]

OCA at 08:40, 26 September 2018

OCA — Wed, 26 Sep 2018 08:40:41 GMT

←Older revision		Revision as of 08:40, 26 September 2018
Line 3:		Line 3:
	<StructureSection load='6e6s' size='340' side='right' caption='[[6e6s]], [[Resolution\|resolution]] 1.45Å' scene=''>		<StructureSection load='6e6s' size='340' side='right' caption='[[6e6s]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6e6s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E6S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6e6s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E6S FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bfd, AO896_12000, AO964_01120, AOY09_04877, C8257_08100, CAZ03_03910, CAZ10_19915, CGU42_05470, CSB93_2785, PAERUG_E15_London_28_01_14_09195, PAMH19_1562, RW109_RW109_02290 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e6s OCA], [http://pdbe.org/6e6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e6s RCSB], [http://www.ebi.ac.uk/pdbsum/6e6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e6s ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e6s OCA], [http://pdbe.org/6e6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e6s RCSB], [http://www.ebi.ac.uk/pdbsum/6e6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e6s ProSAT]</span></td></tr>
	</table>		</table>

OCA at 19:52, 19 September 2018

OCA — Wed, 19 Sep 2018 19:52:45 GMT

←Older revision		Revision as of 19:52, 19 September 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6e6s is ~~ON HOLD until Paper~~ Publication	+	==1.45 A resolution structure of the C-terminally truncated [2Fe-2S] ferredoxin (Bfd) R26E/K46Y mutant from Pseudomonas aeruginosa==
		+	<StructureSection load='6e6s' size='340' side='right' caption='[[6e6s]], [[Resolution\|resolution]] 1.45Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6e6s]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E6S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E6S FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e6s OCA], [http://pdbe.org/6e6s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e6s RCSB], [http://www.ebi.ac.uk/pdbsum/6e6s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e6s ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mobilization of iron from bacterioferritin (BfrB) requires specific interactions with a [2Fe-2S] ferredoxin (Bfd). Blocking the BfrB:Bfd interaction results in irreversible iron accumulation in BfrB and iron deficiency in the cytosol [Eshelman, K., et al. (2017) Metallomics 9, 646-659]. The only known Bfd structure, which was obtained in complex with BfrB (Protein Data Bank entry 4E6K ), indicated a new fold and suggested that the stability of Bfd is aided by an anion binding site consisting of R26, R29, and K46. We investigated the Bfd fold using site-directed mutagenesis, X-ray crystallography, and biochemistry in solution. The X-ray structure, which is nearly identical to that of Bfd in the BfrB:Bfd complex, shows that the [2Fe-2S] cluster preorganizes residues at the BfrB:Bfd interface into a structure complementary to the Bfd binding site on BfrB. Studies in solution showed rapid loss of the [2Fe-2S] cluster at a low ionic strength but higher stability with an increasing ionic strength, thus supporting a structural anion binding site. Structures of the R26E and R26E/K46Y mutants are nearly identical to that of Bfd, except for a new network of hydrogen bonds stabilizing the region encompassing the former anion binding site. The stability of the R26E and R26E/K46Y mutants, which is weakly and completely independent of solution ionic strength, respectively, corroborates that Bfd requires an anion binding site. The mutations, which caused only small changes to the strength of the BfrB:Bfd interaction and mobilization of iron from BfrB, indicate that the anion binding site in Bfd serves primarily a structural role.

-	~~Authors: Lovell~~, S., Wijerathne, H~~., Battaile, K.P.~~, Yao, H., Wang, Y., Rivera, M.	+	Bfd, a New Class of [2Fe-2S] Protein That Functions in Bacterial Iron Homeostasis, Requires a Structural Anion Binding Site.,Wijerathne H, Yao H, Wang Y, Lovell S, Battaile KP, Rivera M Biochemistry. 2018 Sep 13. doi: 10.1021/acs.biochem.8b00823. PMID:30183257<ref>PMID:30183257</ref>

-	~~Description: 1.45 A resolution structure~~ of the C-~~terminally truncated [2Fe~~-~~2S] ferredoxin (Bfd) R26E~~/~~K46Y mutant from Pseudomonas aeruginosa~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	[[Category: Battaile, K.P]]	+	<div class="pdbe-citations 6e6s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Battaile, K P]]
	[[Category: Lovell, S]]		[[Category: Lovell, S]]
	[[Category: Rivera, M]]		[[Category: Rivera, M]]
	[[Category: Wang, Y]]		[[Category: Wang, Y]]
-	[[Category: Yao, H]]
	[[Category: Wijerathne, H]]		[[Category: Wijerathne, H]]
		+	[[Category: Yao, H]]
		+	[[Category: Anion binding site]]
		+	[[Category: Bfd]]
		+	[[Category: Electron transport]]
		+	[[Category: Iron mobilization]]

OCA: Protected "6e6s" [edit=sysop:move=sysop]

OCA — Wed, 01 Aug 2018 17:07:21 GMT

Protected "6e6s" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:07, 1 August 2018

OCA: New page: '''Unreleased structure''' The entry 6e6s is ON HOLD until Paper Publication Authors: Lovell, S., Wijerathne, H., Battaile, K.P., Yao, H., Wang, Y., Rivera, M. Description: 1.45 A reso...

OCA — Wed, 01 Aug 2018 17:07:20 GMT

New page: '''Unreleased structure''' The entry 6e6s is ON HOLD until Paper Publication Authors: Lovell, S., Wijerathne, H., Battaile, K.P., Yao, H., Wang, Y., Rivera, M. Description: 1.45 A reso...

New page

'''Unreleased structure'''

The entry 6e6s is ON HOLD until Paper Publication

Authors: Lovell, S., Wijerathne, H., Battaile, K.P., Yao, H., Wang, Y., Rivera, M.

Description: 1.45 A resolution structure of the C-terminally truncated [2Fe-2S] ferredoxin (Bfd) R26E/K46Y mutant from Pseudomonas aeruginosa
[[Category: Unreleased Structures]]
[[Category: Battaile, K.P]]
[[Category: Lovell, S]]
[[Category: Rivera, M]]
[[Category: Wang, Y]]
[[Category: Yao, H]]
[[Category: Wijerathne, H]]