6eac - Revision history

OCA at 14:40, 13 March 2024

OCA — Wed, 13 Mar 2024 14:40:28 GMT

←Older revision		Revision as of 14:40, 13 March 2024
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	<StructureSection load='6eac' size='340' side='right'caption='[[6eac]], [[Resolution\|resolution]] 2.27Å' scene=''>		<StructureSection load='6eac' size='340' side='right'caption='[[6eac]], [[Resolution\|resolution]] 2.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6eac]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_19310 Atcc 19310~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EAC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6EAC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6eac]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EAC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.269Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6eac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eac OCA], [~~http~~://pdbe.org/6eac PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6eac RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6eac PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6eac ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6eac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eac OCA], [https://pdbe.org/6eac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6eac RCSB], [https://www.ebi.ac.uk/pdbsum/6eac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6eac ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/SELO_PSESM SELO_PSESM] Catalyzes the transfer of adenosine 5'-monophosphate (AMP) to Ser, Thr or Tyr residues of target proteins (AMPylation).[HAMAP-Rule:MF_00692]<ref>PMID:30270044</ref>
-	~~Approximately 10% of human protein kinases are believed to be inactive and named pseudokinases because they lack residues required for catalysis~~. ~~Here, we show that~~ the ~~highly conserved pseudokinase selenoprotein~~-O (~~SelO) transfers~~ AMP ~~from ATP~~ to Ser, Thr~~, and~~ Tyr residues ~~on protein substrates~~ (AMPylation)~~, uncovering a previously unrecognized activity for a member of the protein kinase superfamily~~. ~~The crystal structure of a SelO homolog reveals a protein kinase~~-like fold with ATP flipped in the active site, thus providing a structural basis for catalysis. SelO pseudokinases localize to the mitochondria and AMPylate proteins involved in redox homeostasis. Consequently, SelO activity is necessary for the proper cellular response to oxidative stress. Our results suggest that AMPylation may be a more widespread post-translational modification than previously appreciated and that pseudokinases should be analyzed for alternative transferase activities.	+
-		+
-	Protein AMPylation by an Evolutionarily Conserved Pseudokinase.,Sreelatha A, Yee SS, Lopez VA, Park BC, Kinch LN, Pilch S, Servage KA, Zhang J, Jiou J, Karasiewicz-Urbanska M, Lobocka M, Grishin NV, Orth K, Kucharczyk R, Pawlowski K, Tomchick DR, Tagliabracci VS Cell. 2018 Sep 25. pii: ~~S0092-8674(18)31107-3. doi: 10.1016/j.cell.2018.08.046. PMID:30270044~~<ref>PMID:30270044</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6eac" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 19310]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Sreelatha, A~~]]	+	[[Category: Pseudomonas syringae]]
-	[[Category: ~~Tagliabracci, V S~~]]	+	[[Category: Sreelatha A]]
-	[[Category: ~~Tomchick, D R~~]]	+	[[Category: Tagliabracci VS]]
-	[[Category: ~~Adenylylation]]~~	+	[[Category: Tomchick DR]]
-	~~[[Category: Ampylation]]~~	+
-	~~[[Category: Atypical kinase fold]]~~	+
-	~~[[Category: Flipped atp]]~~	+
-	~~[[Category: Oxidative stress]]~~	+
-	~~[[Category: Pseudokinase]]~~	+
-	~~[[Category: Selenocysteine]]~~	+
-	~~[[Category: Selenoprotein]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 12:54, 25 December 2019

OCA — Wed, 25 Dec 2019 12:54:12 GMT

←Older revision		Revision as of 12:54, 25 December 2019
Line 1:		Line 1:

	==Pseudomonas syringae SelO==		==Pseudomonas syringae SelO==
-	<StructureSection load='6eac' size='340' side='right' caption='[[6eac]], [[Resolution\|resolution]] 2.27Å' scene=''>	+	<StructureSection load='6eac' size='340' side='right'caption='[[6eac]], [[Resolution\|resolution]] 2.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6eac]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19310 Atcc 19310]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EAC FirstGlance]. <br>		<table><tr><td colspan='2'>[[6eac]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19310 Atcc 19310]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EAC FirstGlance]. <br>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 19310]]		[[Category: Atcc 19310]]
		+	[[Category: Large Structures]]
	[[Category: Sreelatha, A]]		[[Category: Sreelatha, A]]
	[[Category: Tagliabracci, V S]]		[[Category: Tagliabracci, V S]]

OCA at 08:37, 17 October 2018

OCA — Wed, 17 Oct 2018 08:37:59 GMT

←Older revision		Revision as of 08:37, 17 October 2018
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eac OCA], [http://pdbe.org/6eac PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eac RCSB], [http://www.ebi.ac.uk/pdbsum/6eac PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eac ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eac OCA], [http://pdbe.org/6eac PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eac RCSB], [http://www.ebi.ac.uk/pdbsum/6eac PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eac ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Approximately 10% of human protein kinases are believed to be inactive and named pseudokinases because they lack residues required for catalysis. Here, we show that the highly conserved pseudokinase selenoprotein-O (SelO) transfers AMP from ATP to Ser, Thr, and Tyr residues on protein substrates (AMPylation), uncovering a previously unrecognized activity for a member of the protein kinase superfamily. The crystal structure of a SelO homolog reveals a protein kinase-like fold with ATP flipped in the active site, thus providing a structural basis for catalysis. SelO pseudokinases localize to the mitochondria and AMPylate proteins involved in redox homeostasis. Consequently, SelO activity is necessary for the proper cellular response to oxidative stress. Our results suggest that AMPylation may be a more widespread post-translational modification than previously appreciated and that pseudokinases should be analyzed for alternative transferase activities.
		+
		+	Protein AMPylation by an Evolutionarily Conserved Pseudokinase.,Sreelatha A, Yee SS, Lopez VA, Park BC, Kinch LN, Pilch S, Servage KA, Zhang J, Jiou J, Karasiewicz-Urbanska M, Lobocka M, Grishin NV, Orth K, Kucharczyk R, Pawlowski K, Tomchick DR, Tagliabracci VS Cell. 2018 Sep 25. pii: S0092-8674(18)31107-3. doi: 10.1016/j.cell.2018.08.046. PMID:30270044<ref>PMID:30270044</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6eac" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:35, 10 October 2018

OCA — Wed, 10 Oct 2018 08:35:20 GMT

←Older revision		Revision as of 08:35, 10 October 2018
Line 3:		Line 3:
	<StructureSection load='6eac' size='340' side='right' caption='[[6eac]], [[Resolution\|resolution]] 2.27Å' scene=''>		<StructureSection load='6eac' size='340' side='right' caption='[[6eac]], [[Resolution\|resolution]] 2.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6eac]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EAC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6eac]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_19310 Atcc 19310]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EAC FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eac OCA], [http://pdbe.org/6eac PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eac RCSB], [http://www.ebi.ac.uk/pdbsum/6eac PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eac ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eac OCA], [http://pdbe.org/6eac PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eac RCSB], [http://www.ebi.ac.uk/pdbsum/6eac PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eac ProSAT]</span></td></tr>
Line 9:		Line 9:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 19310]]
	[[Category: Sreelatha, A]]		[[Category: Sreelatha, A]]
	[[Category: Tagliabracci, V S]]		[[Category: Tagliabracci, V S]]

OCA at 07:49, 3 October 2018

OCA — Wed, 03 Oct 2018 07:49:32 GMT

←Older revision		Revision as of 07:49, 3 October 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6eac ~~is ON HOLD until Paper Publication~~	+	==Pseudomonas syringae SelO==
-		+	<StructureSection load='6eac' size='340' side='right' caption='[[6eac]], [[Resolution\|resolution]] 2.27Å' scene=''>
-	~~Authors~~: ~~Tomchick, D~~.R.~~, Tagliabracci, V~~.S.~~, Sreelatha, A~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6eac]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EAC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EAC FirstGlance]. <br>
-	~~Description: Pseudomonas syringae SelO~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eac OCA], [http://pdbe.org/6eac PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eac RCSB], [http://www.ebi.ac.uk/pdbsum/6eac PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eac ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Tagliabracci~~, V.S]]	+	</table>
-	[[~~Category~~: ~~Tomchick~~, D.R]]	+	__TOC__
		+	</StructureSection>
	[[Category: Sreelatha, A]]		[[Category: Sreelatha, A]]
		+	[[Category: Tagliabracci, V S]]
		+	[[Category: Tomchick, D R]]
		+	[[Category: Adenylylation]]
		+	[[Category: Ampylation]]
		+	[[Category: Atypical kinase fold]]
		+	[[Category: Flipped atp]]
		+	[[Category: Oxidative stress]]
		+	[[Category: Pseudokinase]]
		+	[[Category: Selenocysteine]]
		+	[[Category: Selenoprotein]]
		+	[[Category: Transferase]]

OCA at 14:47, 15 August 2018

OCA — Wed, 15 Aug 2018 14:47:24 GMT

←Older revision		Revision as of 14:47, 15 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6eac is ON HOLD	+	The entry 6eac is ON HOLD until Paper Publication

	Authors: Tomchick, D.R., Tagliabracci, V.S., Sreelatha, A.		Authors: Tomchick, D.R., Tagliabracci, V.S., Sreelatha, A.
Line 7:		Line 7:
	Description: Pseudomonas syringae SelO		Description: Pseudomonas syringae SelO
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Tomchick, D.R]]
	[[Category: Tagliabracci, V.S]]		[[Category: Tagliabracci, V.S]]
		+	[[Category: Tomchick, D.R]]
	[[Category: Sreelatha, A]]		[[Category: Sreelatha, A]]

OCA: Protected "6eac" [edit=sysop:move=sysop]

OCA — Thu, 09 Aug 2018 19:58:44 GMT

Protected "6eac" [edit=sysop:move=sysop]

←Older revision

Revision as of 19:58, 9 August 2018

OCA: New page: '''Unreleased structure''' The entry 6eac is ON HOLD Authors: Tomchick, D.R., Tagliabracci, V.S., Sreelatha, A. Description: Pseudomonas syringae SelO [[Category: Unreleased Structures...

OCA — Thu, 09 Aug 2018 19:58:43 GMT

New page: '''Unreleased structure''' The entry 6eac is ON HOLD Authors: Tomchick, D.R., Tagliabracci, V.S., Sreelatha, A. Description: Pseudomonas syringae SelO [[Category: Unreleased Structures...

New page

'''Unreleased structure'''

The entry 6eac is ON HOLD

Authors: Tomchick, D.R., Tagliabracci, V.S., Sreelatha, A.

Description: Pseudomonas syringae SelO
[[Category: Unreleased Structures]]
[[Category: Tomchick, D.R]]
[[Category: Tagliabracci, V.S]]
[[Category: Sreelatha, A]]