6ebk - Revision history

OCA at 14:40, 13 March 2024

OCA — Wed, 13 Mar 2024 14:40:31 GMT

←Older revision		Revision as of 14:40, 13 March 2024
Line 3:		Line 3:
	<SX load='6ebk' size='340' side='right' viewer='molstar' caption='[[6ebk]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='6ebk' size='340' side='right' viewer='molstar' caption='[[6ebk]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ebk]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6EBK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ebk]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6EBK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6ebk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ebk OCA], [~~http~~://pdbe.org/6ebk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ebk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ebk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ebk ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ebk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ebk OCA], [https://pdbe.org/6ebk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ebk RCSB], [https://www.ebi.ac.uk/pdbsum/6ebk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ebk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KCAB2_RAT KCAB2_RAT]] Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit. [[http://www.uniprot.org/uniprot/KCNA2_RAT KCNA2_RAT]] Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.<ref>PMID:7544443</ref>	+	[https://www.uniprot.org/uniprot/KCAB2_RAT KCAB2_RAT] Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Voltage-activated potassium (Kv) channels open to conduct K(+) ions in response to membrane depolarization, and subsequently enter non-conducting states through distinct mechanisms of inactivation. X-ray structures of detergent-solubilized Kv channels appear to have captured an open state even though a non-conducting C-type inactivated state would predominate in membranes in the absence of a transmembrane voltage. However, structures for a voltage-activated ion channel in a lipid bilayer environment have not yet been reported. Here we report the structure of the Kv1.2-2.1 paddle chimera channel reconstituted into lipid nanodiscs using single-particle cryo-electron microscopy. At a resolution of ~3 A for the cytosolic domain and ~4 A for the transmembrane domain, the structure determined in nanodiscs is similar to the previously determined X-ray structure. Our findings show that large differences in structure between detergent and lipid bilayer environments are unlikely, and enable us to propose possible structural mechanisms for C-type inactivation.	+
-		+
-	Single-particle cryo-EM structure of a voltage-activated potassium channel in lipid nanodiscs.,Matthies D, Bae C, Toombes GE, Fox T, Bartesaghi A, Subramaniam S, Swartz KJ Elife. 2018 Aug 15;7. pii: 37558. doi: 10.7554/eLife.37558. PMID:30109985<ref>PMID:30109985</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6ebk" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]		*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bae, C]]	+	[[Category: Rattus norvegicus]]
-	[[Category: Bartesaghi, A]]	+	[[Category: Bae C]]
-	[[Category: Fox, T]]	+	[[Category: Bartesaghi A]]
-	[[Category: Matthies, D]]	+	[[Category: Fox T]]
-	[[Category: Subramaniam, S]]	+	[[Category: Matthies D]]
-	[[Category: Swartz~~, K J]]~~	+	[[Category: Subramaniam S]]
-	~~[[Category: Lipid nanodisc]]~~	+	[[Category: Swartz KJ]]
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Potassium channel]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 00:23, 11 April 2020

OCA — Sat, 11 Apr 2020 00:23:12 GMT

←Older revision		Revision as of 00:23, 11 April 2020
Line 3:		Line 3:
	<SX load='6ebk' size='340' side='right' viewer='molstar' caption='[[6ebk]], [[Resolution\|resolution]] 3.30Å' scene=''>		<SX load='6ebk' size='340' side='right' viewer='molstar' caption='[[6ebk]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ebk]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6EBK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ebk]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EBK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ebk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ebk OCA], [http://pdbe.org/6ebk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ebk RCSB], [http://www.ebi.ac.uk/pdbsum/6ebk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ebk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ebk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ebk OCA], [http://pdbe.org/6ebk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ebk RCSB], [http://www.ebi.ac.uk/pdbsum/6ebk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ebk ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 21:04, 6 March 2020

OCA — Fri, 06 Mar 2020 21:04:21 GMT

←Older revision		Revision as of 21:04, 6 March 2020
Line 1:		Line 1:

	==The voltage-activated Kv1.2-2.1 paddle chimera channel in lipid nanodiscs==		==The voltage-activated Kv1.2-2.1 paddle chimera channel in lipid nanodiscs==
-	<~~StructureSection~~ load='6ebk' size='340' side='right' caption='[[6ebk]], [[Resolution\|resolution]] 3.30Å' scene=''>	+	<SX load='6ebk' size='340' side='right' viewer='molstar' caption='[[6ebk]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ebk]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EBK FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ebk]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EBK FirstGlance]. <br>
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 6ebk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6ebk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
		+	[[Category: Large Structures]]
	[[Category: Bae, C]]		[[Category: Bae, C]]
	[[Category: Bartesaghi, A]]		[[Category: Bartesaghi, A]]

OCA at 06:09, 22 August 2018

OCA — Wed, 22 Aug 2018 06:09:33 GMT

←Older revision		Revision as of 06:09, 22 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6ebk is ~~ON HOLD~~	+	==The voltage-activated Kv1.2-2.1 paddle chimera channel in lipid nanodiscs==
		+	<StructureSection load='6ebk' size='340' side='right' caption='[[6ebk]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ebk]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EBK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EBK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ebk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ebk OCA], [http://pdbe.org/6ebk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ebk RCSB], [http://www.ebi.ac.uk/pdbsum/6ebk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ebk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KCAB2_RAT KCAB2_RAT]] Accessory potassium channel protein which modulates the activity of the pore-forming alpha subunit. [[http://www.uniprot.org/uniprot/KCNA2_RAT KCNA2_RAT]] Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.<ref>PMID:7544443</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Voltage-activated potassium (Kv) channels open to conduct K(+) ions in response to membrane depolarization, and subsequently enter non-conducting states through distinct mechanisms of inactivation. X-ray structures of detergent-solubilized Kv channels appear to have captured an open state even though a non-conducting C-type inactivated state would predominate in membranes in the absence of a transmembrane voltage. However, structures for a voltage-activated ion channel in a lipid bilayer environment have not yet been reported. Here we report the structure of the Kv1.2-2.1 paddle chimera channel reconstituted into lipid nanodiscs using single-particle cryo-electron microscopy. At a resolution of ~3 A for the cytosolic domain and ~4 A for the transmembrane domain, the structure determined in nanodiscs is similar to the previously determined X-ray structure. Our findings show that large differences in structure between detergent and lipid bilayer environments are unlikely, and enable us to propose possible structural mechanisms for C-type inactivation.

-	~~Authors: Matthies~~, D., Bae, C., ~~Fox~~, T., Bartesaghi, A., Subramaniam, S., Swartz~~, K~~.J.	+	Single-particle cryo-EM structure of a voltage-activated potassium channel in lipid nanodiscs.,Matthies D, Bae C, Toombes GE, Fox T, Bartesaghi A, Subramaniam S, Swartz KJ Elife. 2018 Aug 15;7. pii: 37558. doi: 10.7554/eLife.37558. PMID:30109985<ref>PMID:30109985</ref>

-	~~Description: KV1~~.~~2-KV2~~.~~1 paddle chimera channel in lipid nanodiscs~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Swartz, K.J]]~~	+	<div class="pdbe-citations 6ebk" style="background-color:#fffaf0;"></div>
-	~~[[Category: Subramaniam, S]]~~	+	== References ==
-	~~[[Category: Matthies, D]]~~	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Bae, C]]		[[Category: Bae, C]]
-	[[Category: Fox, T]]
	[[Category: Bartesaghi, A]]		[[Category: Bartesaghi, A]]
		+	[[Category: Fox, T]]
		+	[[Category: Matthies, D]]
		+	[[Category: Subramaniam, S]]
		+	[[Category: Swartz, K J]]
		+	[[Category: Lipid nanodisc]]
		+	[[Category: Membrane protein]]
		+	[[Category: Potassium channel]]
		+	[[Category: Transport protein]]

OCA: Protected "6ebk" [edit=sysop:move=sysop]

OCA — Wed, 15 Aug 2018 14:50:31 GMT

Protected "6ebk" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:50, 15 August 2018

OCA: New page: '''Unreleased structure''' The entry 6ebk is ON HOLD Authors: Matthies, D., Bae, C., Fox, T., Bartesaghi, A., Subramaniam, S., Swartz, K.J. Description: KV1.2-KV2.1 paddle chimera chan...

OCA — Wed, 15 Aug 2018 14:50:30 GMT

New page: '''Unreleased structure''' The entry 6ebk is ON HOLD Authors: Matthies, D., Bae, C., Fox, T., Bartesaghi, A., Subramaniam, S., Swartz, K.J. Description: KV1.2-KV2.1 paddle chimera chan...

New page

'''Unreleased structure'''

The entry 6ebk is ON HOLD

Authors: Matthies, D., Bae, C., Fox, T., Bartesaghi, A., Subramaniam, S., Swartz, K.J.

Description: KV1.2-KV2.1 paddle chimera channel in lipid nanodiscs
[[Category: Unreleased Structures]]
[[Category: Swartz, K.J]]
[[Category: Subramaniam, S]]
[[Category: Matthies, D]]
[[Category: Bae, C]]
[[Category: Fox, T]]
[[Category: Bartesaghi, A]]