6ejf - Revision history

OCA at 00:27, 11 April 2020

OCA — Sat, 11 Apr 2020 00:27:23 GMT

←Older revision		Revision as of 00:27, 11 April 2020
Line 3:		Line 3:
	<SX load='6ejf' size='340' side='right' viewer='molstar' caption='[[6ejf]], [[Resolution\|resolution]] 8.00Å' scene=''>		<SX load='6ejf' size='340' side='right' viewer='molstar' caption='[[6ejf]], [[Resolution\|resolution]] 8.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ejf]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EJF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6EJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ejf]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EJF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EJF FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA0364 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA0364 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ejf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ejf OCA], [http://pdbe.org/6ejf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ejf RCSB], [http://www.ebi.ac.uk/pdbsum/6ejf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ejf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6ejf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ejf OCA], [http://pdbe.org/6ejf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ejf RCSB], [http://www.ebi.ac.uk/pdbsum/6ejf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ejf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:05, 6 March 2020

OCA — Fri, 06 Mar 2020 21:05:56 GMT

←Older revision		Revision as of 21:05, 6 March 2020
Line 1:		Line 1:

	==Thermus thermophilus PilF ATPase (apoprotein form)==		==Thermus thermophilus PilF ATPase (apoprotein form)==
-	<~~StructureSection~~ load='6ejf' size='340' side='right' caption='[[6ejf]], [[Resolution\|resolution]] 8.00Å' scene=''>	+	<SX load='6ejf' size='340' side='right' viewer='molstar' caption='[[6ejf]], [[Resolution\|resolution]] 8.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ejf]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EJF FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ejf]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EJF FirstGlance]. <br>
Line 19:		Line 19:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
		+	[[Category: Large Structures]]
	[[Category: Thet8]]		[[Category: Thet8]]
	[[Category: Collins, R F]]		[[Category: Collins, R F]]

OCA at 06:53, 24 October 2018

OCA — Wed, 24 Oct 2018 06:53:43 GMT

←Older revision		Revision as of 06:53, 24 October 2018
Line 3:		Line 3:
	<StructureSection load='6ejf' size='340' side='right' caption='[[6ejf]], [[Resolution\|resolution]] 8.00Å' scene=''>		<StructureSection load='6ejf' size='340' side='right' caption='[[6ejf]], [[Resolution\|resolution]] 8.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ejf]] is a 18 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ejf]] is a 18 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EJF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ejf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ejf OCA], [http://pdbe.org/6ejf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ejf RCSB], [http://www.ebi.ac.uk/pdbsum/6ejf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ejf ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TTHA0364 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ejf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ejf OCA], [http://pdbe.org/6ejf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ejf RCSB], [http://www.ebi.ac.uk/pdbsum/6ejf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ejf ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Type IV pili are responsible for a diverse range of functions, including twitching motility and cell adhesion. Assembly of the pilus fiber is driven by a cytoplasmic ATPase: it interacts with an inner membrane complex of biogenesis proteins which, in turn, bind to nascent pilin subunits and mediate fiber assembly. Here we report the structural characterization of the PilF TFP assembly ATPase from Thermus thermophilus. The crystal structure of a recombinant C-terminal fragment of PilF revealed bound, unhydrolysed ATP, although the full length complex was enzymatically active. 3D reconstructions were carried out by single particle cryoelectron microscopy for full length apoprotein PilF and in complex with AMPPNP. The structure forms an hourglass-like shape, with the ATPase domains in one half and the N1 domains in the second half which, we propose, interact with the other pilus biogenesis components. Molecular models for both forms were generated: binding of AMPPNP causes an upward shift of the N1 domains towards the ATPase domains of ~8 A. We advocate a model in which ATP hydrolysis is linked to displacement of the N1 domains which is associated with lifting pilin subunits out of the inner membrane, and provide the activation energy needed to form the pilus fiber.
		+
		+	Structural cycle of the Thermus thermophilus PilF ATPase: the powering of type IVa pilus assembly.,Collins R, Karuppiah V, Siebert CA, Dajani R, Thistlethwaite A, Derrick JP Sci Rep. 2018 Sep 19;8(1):14022. doi: 10.1038/s41598-018-32218-3. PMID:30232337<ref>PMID:30232337</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ejf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Thet8]]
	[[Category: Collins, R F]]		[[Category: Collins, R F]]
	[[Category: Derrick, J P]]		[[Category: Derrick, J P]]

OCA at 18:52, 1 August 2018

OCA — Wed, 01 Aug 2018 18:52:58 GMT

←Older revision		Revision as of 18:52, 1 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6ejf ~~is ON HOLD~~	+	==Thermus thermophilus PilF ATPase (apoprotein form)==
-		+	<StructureSection load='6ejf' size='340' side='right' caption='[[6ejf]], [[Resolution\|resolution]] 8.00Å' scene=''>
-	~~Authors~~: ~~Derrick, J~~.P.~~, Collins, R~~.F.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ejf]] is a 18 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EJF FirstGlance]. <br>
-	~~Description~~: ~~Thermus thermophilus PilF ATPase (apoprotein form)~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ejf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ejf OCA], [http://pdbe.org/6ejf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ejf RCSB], [http://www.ebi.ac.uk/pdbsum/6ejf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ejf ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Collins, R.F]]	+	__TOC__
-	[[Category: Derrick, J.P]]	+	</StructureSection>
		+	[[Category: Collins, R F]]
		+	[[Category: Derrick, J P]]
		+	[[Category: Macromolecular machine]]
		+	[[Category: Motor protein]]
		+	[[Category: Type ii secretion]]
		+	[[Category: Type iv pilus]]

OCA at 05:07, 28 February 2018

OCA — Wed, 28 Feb 2018 05:07:16 GMT

←Older revision		Revision as of 05:07, 28 February 2018
Line 5:		Line 5:
	Authors: Derrick, J.P., Collins, R.F.		Authors: Derrick, J.P., Collins, R.F.

-	Description: Thermus thermophilus PilF ATPase (~~AMPPNP-bound~~ form)	+	Description: Thermus thermophilus PilF ATPase (apoprotein form)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Collins, R.F]]		[[Category: Collins, R.F]]
	[[Category: Derrick, J.P]]		[[Category: Derrick, J.P]]

OCA at 17:23, 24 January 2018

OCA — Wed, 24 Jan 2018 17:23:51 GMT

←Older revision		Revision as of 17:23, 24 January 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ejf is ON HOLD ~~until sometime in the future~~	+	The entry 6ejf is ON HOLD

	Authors: Derrick, J.P., Collins, R.F.		Authors: Derrick, J.P., Collins, R.F.

OCA at 05:40, 11 October 2017

OCA — Wed, 11 Oct 2017 05:40:25 GMT

←Older revision		Revision as of 05:40, 11 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ejf is ON HOLD	+	The entry 6ejf is ON HOLD until sometime in the future

	Authors: Derrick, J.P., Collins, R.F.		Authors: Derrick, J.P., Collins, R.F.

OCA: Protected "6ejf" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 17:14:47 GMT

Protected "6ejf" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:14, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6ejf is ON HOLD Authors: Derrick, J.P., Collins, R.F. Description: Thermus thermophilus PilF ATPase (AMPPNP-bound form) [[Category: Unreleased Stru...

OCA — Wed, 27 Sep 2017 17:14:46 GMT

New page: '''Unreleased structure''' The entry 6ejf is ON HOLD Authors: Derrick, J.P., Collins, R.F. Description: Thermus thermophilus PilF ATPase (AMPPNP-bound form) [[Category: Unreleased Stru...

New page

'''Unreleased structure'''

The entry 6ejf is ON HOLD

Authors: Derrick, J.P., Collins, R.F.

Description: Thermus thermophilus PilF ATPase (AMPPNP-bound form)
[[Category: Unreleased Structures]]
[[Category: Collins, R.F]]
[[Category: Derrick, J.P]]