OCA at 07:53, 12 September 2018

OCA — Wed, 12 Sep 2018 07:53:34 GMT

←Older revision		Revision as of 07:53, 12 September 2018
Line 3:		Line 3:
	<StructureSection load='6el3' size='340' side='right' caption='[[6el3]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6el3' size='340' side='right' caption='[[6el3]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6el3]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EL3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EL3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6el3]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EL3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EL3 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">VEP1, AWI31, At4g24220, T22A6.50 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Delta(4)-3-oxosteroid_5-beta-reductase Delta(4)-3-oxosteroid 5-beta-reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.3 1.3.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Delta(4)-3-oxosteroid_5-beta-reductase Delta(4)-3-oxosteroid 5-beta-reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.3 1.3.1.3] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6el3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6el3 OCA], [http://pdbe.org/6el3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6el3 RCSB], [http://www.ebi.ac.uk/pdbsum/6el3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6el3 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6el3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6el3 OCA], [http://pdbe.org/6el3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6el3 RCSB], [http://www.ebi.ac.uk/pdbsum/6el3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6el3 ProSAT]</span></td></tr>
Line 11:		Line 12:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/VEP1_ARATH VEP1_ARATH]] Involved in vascular strand development. Catalyzes the stereospecific conversion of progesterone to 5-beta-pregnane-3,20-dione. Can use progesterone, testosterone, 21-acetyl cortexone, 2-cyclohexenone, but-1-en-3-one, ethyl acrylate, ethylmethacrylate, cortisone and canarigenone as substrates, lower activity with 3-methyl-2-cyclohexenone and 3,5,5-trimethyl-2-cyclohexenone as substrate, and no activity with canarigenin, canarigenin digitoxoside and pregnenolone. May be involved in the formation of 5-beta phytoecdysteroids.<ref>PMID:11917087</ref> <ref>PMID:19166903</ref>		[[http://www.uniprot.org/uniprot/VEP1_ARATH VEP1_ARATH]] Involved in vascular strand development. Catalyzes the stereospecific conversion of progesterone to 5-beta-pregnane-3,20-dione. Can use progesterone, testosterone, 21-acetyl cortexone, 2-cyclohexenone, but-1-en-3-one, ethyl acrylate, ethylmethacrylate, cortisone and canarigenone as substrates, lower activity with 3-methyl-2-cyclohexenone and 3,5,5-trimethyl-2-cyclohexenone as substrate, and no activity with canarigenin, canarigenin digitoxoside and pregnenolone. May be involved in the formation of 5-beta phytoecdysteroids.<ref>PMID:11917087</ref> <ref>PMID:19166903</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PRISEs (progesterone 5beta-reductase and/or iridoid synthase-like 1,4-enone reductases) are involved in cardenolide and iridoid biosynthesis. We here investigated a PRISE (rAtSt5betaR) from Arabidopsis thaliana, a plant producing neither cardenolides nor iridoids. The structure of rAtSt5betaR was elucidated with X-ray crystallography and compared to the known structures of PRISEs from Catharanthus roseus (rCrISY) and Digitalis lanata (rDlP5betaR). The three enzymes show a high degree of sequence and structure conservation in the active site. Amino acids previously considered to allow discrimination between progesterone 5beta-reductase and iridoid synthase were interchanged among rAtSt5betaR, rCrISY and rDlP5betaR applying site-directed mutagenesis. Structural homologous substitutions had different effects, and changes in progesterone 5beta-reductase and iridoid synthase activity were not correlated in all cases. Our results help to explain fortuitous emergence of metabolic pathways and product accumulation. The fact that PRISEs are found ubiquitously in spermatophytes insinuates that PRISEs might have a more general function in plant metabolism such as, for example, the detoxification of reactive carbonyl species.
		+
		+	PRISEs (progesterone 5beta-reductase and/or iridoid synthase-like 1,4-enone reductases): Catalytic and substrate promiscuity allows for realization of multiple pathways in plant metabolism.,Schmidt K, Petersen J, Munkert J, Egerer-Sieber C, Hornig M, Muller YA, Kreis W Phytochemistry. 2018 Aug 29;156:9-19. doi: 10.1016/j.phytochem.2018.08.012. PMID:30172078<ref>PMID:30172078</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6el3" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arath]]
	[[Category: Egerer-Sieber, C]]		[[Category: Egerer-Sieber, C]]
	[[Category: Muller, Y A]]		[[Category: Muller, Y A]]

OCA at 10:11, 5 September 2018

OCA — Wed, 05 Sep 2018 10:11:26 GMT

←Older revision		Revision as of 10:11, 5 September 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6el3 is ~~ON HOLD~~	+	==Structure of Progesterone 5beta-Reductase from Arabidopsis thaliana in complex with NADP==
-		+	<StructureSection load='6el3' size='340' side='right' caption='[[6el3]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6el3]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EL3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EL3 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Delta(4)-3-oxosteroid_5-beta-reductase Delta(4)-3-oxosteroid 5-beta-reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.3 1.3.1.3] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6el3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6el3 OCA], [http://pdbe.org/6el3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6el3 RCSB], [http://www.ebi.ac.uk/pdbsum/6el3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6el3 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/VEP1_ARATH VEP1_ARATH]] Involved in vascular strand development. Catalyzes the stereospecific conversion of progesterone to 5-beta-pregnane-3,20-dione. Can use progesterone, testosterone, 21-acetyl cortexone, 2-cyclohexenone, but-1-en-3-one, ethyl acrylate, ethylmethacrylate, cortisone and canarigenone as substrates, lower activity with 3-methyl-2-cyclohexenone and 3,5,5-trimethyl-2-cyclohexenone as substrate, and no activity with canarigenin, canarigenin digitoxoside and pregnenolone. May be involved in the formation of 5-beta phytoecdysteroids.<ref>PMID:11917087</ref> <ref>PMID:19166903</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Egerer-Sieber, C]]
		+	[[Category: Muller, Y A]]
		+	[[Category: Schmidt, K]]
		+	[[Category: Homodimer]]
		+	[[Category: Nadp binding site]]
		+	[[Category: Oxidoreductase]]

OCA: Protected "6el3" [edit=sysop:move=sysop]

OCA — Wed, 04 Oct 2017 08:20:09 GMT

Protected "6el3" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:20, 4 October 2017

OCA: New page: '''Unreleased structure''' The entry 6el3 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 04 Oct 2017 08:20:08 GMT

New page: '''Unreleased structure''' The entry 6el3 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6el3 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6el3 - Revision history

OCA at 07:53, 12 September 2018

OCA at 10:11, 5 September 2018

OCA: Protected "6el3" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6el3 is ON HOLD Authors: Description: Category: Unreleased Structures