6en1 - Revision history

OCA at 09:12, 11 November 2020

OCA — Wed, 11 Nov 2020 09:12:42 GMT

←Older revision		Revision as of 09:12, 11 November 2020
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	==Structure of the Tn1549 transposon Integrase (aa 82-397, R225K) in complex with a circular intermediate DNA (CI6a-DNA)==		==Structure of the Tn1549 transposon Integrase (aa 82-397, R225K) in complex with a circular intermediate DNA (CI6a-DNA)==
-	<StructureSection load='6en1' size='340' side='right' caption='[[6en1]], [[Resolution\|resolution]] 2.67Å' scene=''>	+	<StructureSection load='6en1' size='340' side='right'caption='[[6en1]], [[Resolution\|resolution]] 2.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6en1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EN1 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6EN1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6en1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EN1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EN1 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6emy\|6emy]], [[6emz\|6emz]], [[6en0\|6en0]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6emy\|6emy]], [[6emz\|6emz]], [[6en0\|6en0]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6en1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6en1 OCA], [http://pdbe.org/6en1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6en1 RCSB], [http://www.ebi.ac.uk/pdbsum/6en1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6en1 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6en1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6en1 OCA], [http://pdbe.org/6en1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6en1 RCSB], [http://www.ebi.ac.uk/pdbsum/6en1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6en1 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 6en1" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6en1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Retroviral integrase 3D structures\|Retroviral integrase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Barabas, O]]		[[Category: Barabas, O]]
	[[Category: Rubio-Cosials, A]]		[[Category: Rubio-Cosials, A]]

OCA at 06:01, 4 April 2018

OCA — Wed, 04 Apr 2018 06:01:17 GMT

←Older revision		Revision as of 06:01, 4 April 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6en1 is ~~ON HOLD until Paper~~ Publication	+	==Structure of the Tn1549 transposon Integrase (aa 82-397, R225K) in complex with a circular intermediate DNA (CI6a-DNA)==
		+	<StructureSection load='6en1' size='340' side='right' caption='[[6en1]], [[Resolution\|resolution]] 2.67Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6en1]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EN1 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6emy\|6emy]], [[6emz\|6emz]], [[6en0\|6en0]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6en1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6en1 OCA], [http://pdbe.org/6en1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6en1 RCSB], [http://www.ebi.ac.uk/pdbsum/6en1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6en1 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Conjugative transposition drives the emergence of multidrug resistance in diverse bacterial pathogens, yet the mechanisms are poorly characterized. The Tn1549 conjugative transposon propagates resistance to the antibiotic vancomycin used for severe drug-resistant infections. Here, we present four high-resolution structures of the conserved Y-transposase of Tn1549 complexed with circular transposon DNA intermediates. The structures reveal individual transposition steps and explain how specific DNA distortion and cleavage mechanisms enable DNA strand exchange with an absolute minimum homology requirement. This appears to uniquely allow Tn916-like conjugative transposons to bypass DNA homology and insert into diverse genomic sites, expanding gene transfer. We further uncover a structural regulatory mechanism that prevents premature cleavage of the transposon DNA before a suitable target DNA is found and generate a peptide antagonist that interferes with the transposase-DNA structure to block transposition. Our results reveal mechanistic principles of conjugative transposition that could help control the spread of antibiotic resistance genes.

-	~~Authors:~~ Rubio-Cosials, A., ~~Barabas~~, O.	+	Transposase-DNA Complex Structures Reveal Mechanisms for Conjugative Transposition of Antibiotic Resistance.,Rubio-Cosials A, Schulz EC, Lambertsen L, Smyshlyaev G, Rojas-Cordova C, Forslund K, Karaca E, Bebel A, Bork P, Barabas O Cell. 2018 Mar 22;173(1):208-220.e20. doi: 10.1016/j.cell.2018.02.032. Epub 2018 , Mar 15. PMID:29551265<ref>PMID:29551265</ref>

-	~~Description: Structure~~ of the ~~Tn1549 transposon Integrase (aa 82~~-~~397, R225K) in complex with a circular intermediate DNA (CI6a~~-~~DNA)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Rubio-Cosials, A]]~~	+	<div class="pdbe-citations 6en1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Barabas, O]]		[[Category: Barabas, O]]
		+	[[Category: Rubio-Cosials, A]]
		+	[[Category: Antibiotic resistance transfer]]
		+	[[Category: Recombination]]
		+	[[Category: Tn916-like conjugative transposon]]
		+	[[Category: Transposase protein - dna complex]]
		+	[[Category: Tyrosine recombinase]]
		+	[[Category: Y-transposase]]

OCA at 05:03, 1 November 2017

OCA — Wed, 01 Nov 2017 05:03:22 GMT

←Older revision		Revision as of 05:03, 1 November 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6en1 is ON HOLD	+	The entry 6en1 is ON HOLD until Paper Publication

	Authors: Rubio-Cosials, A., Barabas, O.		Authors: Rubio-Cosials, A., Barabas, O.

OCA: Protected "6en1" [edit=sysop:move=sysop]

OCA — Wed, 11 Oct 2017 05:44:49 GMT

Protected "6en1" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:44, 11 October 2017

OCA: New page: '''Unreleased structure''' The entry 6en1 is ON HOLD Authors: Rubio-Cosials, A., Barabas, O. Description: Structure of the Tn1549 transposon Integrase (aa 82-397, R225K) in complex wit...

OCA — Wed, 11 Oct 2017 05:44:48 GMT

New page: '''Unreleased structure''' The entry 6en1 is ON HOLD Authors: Rubio-Cosials, A., Barabas, O. Description: Structure of the Tn1549 transposon Integrase (aa 82-397, R225K) in complex wit...

New page

'''Unreleased structure'''

The entry 6en1 is ON HOLD

Authors: Rubio-Cosials, A., Barabas, O.

Description: Structure of the Tn1549 transposon Integrase (aa 82-397, R225K) in complex with a circular intermediate DNA (CI6a-DNA)
[[Category: Unreleased Structures]]
[[Category: Rubio-Cosials, A]]
[[Category: Barabas, O]]