6en3 - Revision history

OCA at 09:12, 11 November 2020

OCA — Wed, 11 Nov 2020 09:12:51 GMT

←Older revision		Revision as of 09:12, 11 November 2020
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	==Crystal structure of full length EndoS from Streptococcus pyogenes in complex with G2 oligosaccharide.==		==Crystal structure of full length EndoS from Streptococcus pyogenes in complex with G2 oligosaccharide.==
-	<StructureSection load='6en3' size='340' side='right' caption='[[6en3]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='6en3' size='340' side='right'caption='[[6en3]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6en3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EN3 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6EN3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6en3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibch Vibch]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EN3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6EN3 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <~~scene name~~='~~pdbligand~~=NI:~~NICKEL+(II)+ION'~~>NI</~~scene~~></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6en3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6en3 OCA], [http://pdbe.org/6en3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6en3 RCSB], [http://www.ebi.ac.uk/pdbsum/6en3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6en3 ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">rtxA, rtx, VC_1451 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 VIBCH])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6en3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6en3 OCA], [http://pdbe.org/6en3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6en3 RCSB], [http://www.ebi.ac.uk/pdbsum/6en3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6en3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Vibch]]
	[[Category: Guerin, M E]]		[[Category: Guerin, M E]]
	[[Category: Klontz, E H]]		[[Category: Klontz, E H]]

OCA at 07:41, 14 June 2018

OCA — Thu, 14 Jun 2018 07:41:36 GMT

←Older revision		Revision as of 07:41, 14 June 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6en3 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of full length EndoS from Streptococcus pyogenes in complex with G2 oligosaccharide.==
		+	<StructureSection load='6en3' size='340' side='right' caption='[[6en3]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6en3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EN3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EN3 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6en3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6en3 OCA], [http://pdbe.org/6en3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6en3 RCSB], [http://www.ebi.ac.uk/pdbsum/6en3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6en3 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Endoglycosidase S (EndoS) is a bacterial endo-beta-N-acetylglucosaminidase that specifically catalyzes the hydrolysis of the beta-1,4 linkage between the first two N-acetylglucosamine residues of the biantennary complex-type N-linked glycans of IgG Fc regions. It is used for the chemoenzymatic synthesis of homogeneously glycosylated antibodies with improved therapeutic properties, but the molecular basis for its substrate specificity is unknown. Here, we report the crystal structure of the full-length EndoS in complex with its oligosaccharide G2 product. The glycoside hydrolase domain contains two well-defined asymmetric grooves that accommodate the complex-type N-linked glycan antennae near the active site. Several loops shape the glycan binding site, thereby governing the strict substrate specificity of EndoS. Comparing the arrangement of these loops within EndoS and related endoglycosidases, reveals distinct-binding site architectures that correlate with the respective glycan specificities, providing a basis for the bioengineering of endoglycosidases to tailor the chemoenzymatic synthesis of monoclonal antibodies.

-	~~Authors~~:	+	Structural basis for the recognition of complex-type N-glycans by Endoglycosidase S.,Trastoy B, Klontz E, Orwenyo J, Marina A, Wang LX, Sundberg EJ, Guerin ME Nat Commun. 2018 May 14;9(1):1874. doi: 10.1038/s41467-018-04300-x. PMID:29760474<ref>PMID:29760474</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6en3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Guerin, M E]]
		+	[[Category: Klontz, E H]]
		+	[[Category: Marina, A]]
		+	[[Category: Orwenyo, J]]
		+	[[Category: Sundberg, E J]]
		+	[[Category: Trastoy, B]]
		+	[[Category: Wang, L X]]
		+	[[Category: Endoglycosidase/immunoglobulin/carbohydrate/endo-beta-n-acetylglucos aminidase]]
		+	[[Category: Hydrolase]]

OCA at 05:54, 25 October 2017

OCA — Wed, 25 Oct 2017 05:54:40 GMT

←Older revision		Revision as of 05:54, 25 October 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6en3 is ON HOLD	+	The entry 6en3 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6en3" [edit=sysop:move=sysop]

OCA — Wed, 11 Oct 2017 05:44:56 GMT

Protected "6en3" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:44, 11 October 2017

OCA: New page: '''Unreleased structure''' The entry 6en3 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 11 Oct 2017 05:44:55 GMT

New page: '''Unreleased structure''' The entry 6en3 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6en3 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]