6eny - Revision history

OCA at 00:30, 11 April 2020

OCA — Sat, 11 Apr 2020 00:30:26 GMT

←Older revision		Revision as of 00:30, 11 April 2020
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	<SX load='6eny' size='340' side='right' viewer='molstar' caption='[[6eny]], [[Resolution\|resolution]] 5.80Å' scene=''>		<SX load='6eny' size='340' side='right' viewer='molstar' caption='[[6eny]], [[Resolution\|resolution]] 5.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6eny]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ENY OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ENY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6eny]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ENY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ENY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_disulfide-isomerase Protein disulfide-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.4.1 5.3.4.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_disulfide-isomerase Protein disulfide-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.4.1 5.3.4.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6eny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eny OCA], [http://pdbe.org/6eny PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eny RCSB], [http://www.ebi.ac.uk/pdbsum/6eny PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eny ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6eny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eny OCA], [http://pdbe.org/6eny PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eny RCSB], [http://www.ebi.ac.uk/pdbsum/6eny PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eny ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==

OCA at 21:08, 6 March 2020

OCA — Fri, 06 Mar 2020 21:08:47 GMT

←Older revision		Revision as of 21:08, 6 March 2020
Line 1:		Line 1:

	==Structure of the human PLC editing module==		==Structure of the human PLC editing module==
-	<~~StructureSection~~ load='6eny' size='340' side='right' caption='[[6eny]], [[Resolution\|resolution]] 5.80Å' scene=''>	+	<SX load='6eny' size='340' side='right' viewer='molstar' caption='[[6eny]], [[Resolution\|resolution]] 5.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6eny]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ENY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ENY FirstGlance]. <br>		<table><tr><td colspan='2'>[[6eny]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ENY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ENY FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 6eny" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6eny" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Beta-2 microglobulin 3D structures\|Beta-2 microglobulin 3D structures]]
		+	*[[Calreticulin\|Calreticulin]]
		+	*[[Calreticulin 3D structures\|Calreticulin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
	[[Category: Protein disulfide-isomerase]]		[[Category: Protein disulfide-isomerase]]
	[[Category: Blees, A]]		[[Category: Blees, A]]

OCA: Protected "6eny" [edit=sysop:move=sysop]

OCA — Wed, 29 Nov 2017 06:16:53 GMT

Protected "6eny" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:16, 29 November 2017

OCA: New page: ==Structure of the human PLC editing module== == Structural highl...

OCA — Wed, 29 Nov 2017 06:16:50 GMT

New page: ==Structure of the human PLC editing module== <StructureSection load='6eny' size='340' side='right' caption='6eny, resolution 5.80Å' scene=''> == Structural highl...

New page

==Structure of the human PLC editing module==
<StructureSection load='6eny' size='340' side='right' caption='[[6eny]], [[Resolution|resolution]] 5.80Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6eny]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ENY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ENY FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein_disulfide-isomerase Protein disulfide-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.4.1 5.3.4.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6eny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6eny OCA], [http://pdbe.org/6eny PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6eny RCSB], [http://www.ebi.ac.uk/pdbsum/6eny PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6eny ProSAT]</span></td></tr>
</table>
== Disease ==
[[http://www.uniprot.org/uniprot/B2MG_HUMAN B2MG_HUMAN]] Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:[http://omim.org/entry/241600 241600]]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.<ref>PMID:16549777</ref> Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.<ref>PMID:3532124</ref> <ref>PMID:1336137</ref> <ref>PMID:7554280</ref> <ref>PMID:4586824</ref> <ref>PMID:8084451</ref> <ref>PMID:12119416</ref> <ref>PMID:12796775</ref> <ref>PMID:16901902</ref> <ref>PMID:16491088</ref> <ref>PMID:17646174</ref> <ref>PMID:18835253</ref> <ref>PMID:18395224</ref> <ref>PMID:19284997</ref>
== Function ==
[[http://www.uniprot.org/uniprot/1A03_HUMAN 1A03_HUMAN]] Involved in the presentation of foreign antigens to the immune system. [[http://www.uniprot.org/uniprot/B2MG_HUMAN B2MG_HUMAN]] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. [[http://www.uniprot.org/uniprot/TPSN_HUMAN TPSN_HUMAN]] Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).<ref>PMID:10636848</ref> [[http://www.uniprot.org/uniprot/CALR_HUMAN CALR_HUMAN]] Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity).<ref>PMID:7876246</ref> <ref>PMID:11149926</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The peptide-loading complex (PLC) is a transient, multisubunit membrane complex in the endoplasmic reticulum that is essential for establishing a hierarchical immune response. The PLC coordinates peptide translocation into the endoplasmic reticulum with loading and editing of major histocompatibility complex class I (MHC-I) molecules. After final proofreading in the PLC, stable peptide-MHC-I complexes are released to the cell surface to evoke a T-cell response against infected or malignant cells. Sampling of different MHC-I allomorphs requires the precise coordination of seven different subunits in a single macromolecular assembly, including the transporter associated with antigen processing (TAP1 and TAP2, jointly referred to as TAP), the oxidoreductase ERp57, the MHC-I heterodimer, and the chaperones tapasin and calreticulin. The molecular organization of and mechanistic events that take place in the PLC are unknown owing to the heterogeneous composition and intrinsically dynamic nature of the complex. Here, we isolate human PLC from Burkitt's lymphoma cells using an engineered viral inhibitor as bait and determine the structure of native PLC by electron cryo-microscopy. Two endoplasmic reticulum-resident editing modules composed of tapasin, calreticulin, ERp57, and MHC-I are centred around TAP in a pseudo-symmetric orientation. A multivalent chaperone network within and across the editing modules establishes the proofreading function at two lateral binding platforms for MHC-I molecules. The lectin-like domain of calreticulin senses the MHC-I glycan, whereas the P domain reaches over the MHC-I peptide-binding pocket towards ERp57. This arrangement allows tapasin to facilitate peptide editing by clamping MHC-I. The translocation pathway of TAP opens out into a large endoplasmic reticulum lumenal cavity, confined by the membrane entry points of tapasin and MHC-I. Two lateral windows channel the antigenic peptides to MHC-I. Structures of PLC captured at distinct assembly states provide mechanistic insight into the recruitment and release of MHC-I. Our work defines the molecular symbiosis of an ABC transporter and an endoplasmic reticulum chaperone network in MHC-I assembly and provides insight into the onset of the adaptive immune response.

Structure of the human MHC-I peptide-loading complex.,Blees A, Januliene D, Hofmann T, Koller N, Schmidt C, Trowitzsch S, Moeller A, Tampe R Nature. 2017 Nov 23;551(7681):525-528. doi: 10.1038/nature24627. Epub 2017 Nov 6. PMID:29107940<ref>PMID:29107940</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6eny" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Protein disulfide-isomerase]]
[[Category: Blees, A]]
[[Category: Januliene, D]]
[[Category: Moeller, A]]
[[Category: Tampe, R]]
[[Category: Trowitzsch, S]]
[[Category: Adaptive immunity]]
[[Category: Antigen processing]]
[[Category: Chaperone]]
[[Category: Immune system]]
[[Category: Mhc class i]]