6esz - Revision history

OCA at 06:55, 24 October 2018

OCA — Wed, 24 Oct 2018 06:55:00 GMT

←Older revision		Revision as of 06:55, 24 October 2018
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	<StructureSection load='6esz' size='340' side='right' caption='[[6esz]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='6esz' size='340' side='right' caption='[[6esz]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6esz]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ESZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6esz]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ESZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">pqsC, PA0998 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE]), pqsB, PA0997 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6esz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esz OCA], [http://pdbe.org/6esz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esz RCSB], [http://www.ebi.ac.uk/pdbsum/6esz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6esz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esz OCA], [http://pdbe.org/6esz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esz RCSB], [http://www.ebi.ac.uk/pdbsum/6esz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esz ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pseae]]
	[[Category: Blankenfeldt, W]]		[[Category: Blankenfeldt, W]]
	[[Category: Witzgall, F]]		[[Category: Witzgall, F]]

OCA at 07:18, 4 July 2018

OCA — Wed, 04 Jul 2018 07:18:38 GMT

←Older revision		Revision as of 07:18, 4 July 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6esz is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of PqsBC from Pseudomonas aeruginosa (crystal form 1)==
		+	<StructureSection load='6esz' size='340' side='right' caption='[[6esz]], [[Resolution\|resolution]] 1.84Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6esz]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ESZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ESZ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6esz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6esz OCA], [http://pdbe.org/6esz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6esz RCSB], [http://www.ebi.ac.uk/pdbsum/6esz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6esz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PQSC_PSEAE PQSC_PSEAE]] Required for the biosynthesis of the quorum-sensing signaling molecules 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)-quinolone (Pseudomonas quinolone signal or PQS), which are important for biofilm formation and virulence. The PqsC/PqsB complex catalyzes the condensation of 2-aminobenzoylacetate (2-ABA) and octanoyl-CoA to form HHQ. First, PqsC acquires an octanoyl group from octanoyl-CoA and forms an octanoyl-PqsC intermediate. Then, together with PqsB, it catalyzes the coupling of 2-ABA with the octanoate group, leading to decarboxylation and dehydration, and resulting in closure of the quinoline ring.<ref>PMID:24239007</ref> <ref>PMID:26811339</ref> [[http://www.uniprot.org/uniprot/PQSB_PSEAE PQSB_PSEAE]] Required for the biosynthesis of the quorum-sensing signaling molecules 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)-quinolone (Pseudomonas quinolone signal or PQS), which are important for biofilm formation and virulence. The PqsC/PqsB complex catalyzes the condensation of 2-aminobenzoylacetate (2-ABA) and octanoyl-CoA to form HHQ. PqsB, together with PqsC, catalyzes the coupling of 2-ABA with the octanoate group, leading to decarboxylation and dehydration, and resulting in closure of the quinoline ring. PqsB is probably required for the proper folding of PqsC rather than for a direct enzymatic role in the process.<ref>PMID:24239007</ref> <ref>PMID:26811339</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pseudomonas aeruginosa is a bacterial pathogen that causes life-threatening infections in immunocompromised patients. It produces a large armory of saturated and mono-unsaturated 2-alkyl-4(1H)-quinolones (AQs) and AQ N-oxides (AQNOs) that serve as signaling molecules to control the production of virulence factors and that are involved in membrane vesicle formation and iron chelation; furthermore, they also have, for example, antibiotic properties. It has been shown that the beta-ketoacyl-acyl-carrier protein synthase III (FabH)-like heterodimeric enzyme PqsBC catalyzes the last step in the biosynthesis of the most abundant AQ congener, 2-heptyl-4(1H)-quinolone (HHQ), by condensing octanoyl-coenzyme A (CoA) with 2-aminobenzoylacetate (2-ABA), but the basis for the large number of other AQs/AQNOs produced by P. aeruginosa is not known. Here, we demonstrate that PqsBC uses different medium-chain acyl-CoAs to produce various saturated AQs/AQNOs and that it also biosynthesizes mono-unsaturated congeners. Further, we determined the structures of PqsBC in four different crystal forms at 1.5 to 2.7 A resolution. Together with a previous report, the data reveal that PqsBC adopts open, intermediate, and closed conformations that alter the shape of the acyl-binding cavity and explain the promiscuity of PqsBC. The different conformations also allow us to propose a model for structural transitions that accompany the catalytic cycle of PqsBC that might have broader implications for other FabH-enzymes, for which such structural transitions have been postulated but have never been observed.

-	~~Authors~~:	+	The Alkylquinolone Repertoire of Pseudomonas aeruginosa is Linked to Structural Flexibility of the FabH-like 2-Heptyl-3-hydroxy-4(1H)-quinolone (PQS) Biosynthesis Enzyme PqsBC.,Witzgall F, Depke T, Hoffmann M, Empting M, Bronstrup M, Muller R, Blankenfeldt W Chembiochem. 2018 May 3. doi: 10.1002/cbic.201800153. PMID:29722462<ref>PMID:29722462</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6esz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Blankenfeldt, W]]
		+	[[Category: Witzgall, F]]
		+	[[Category: Fabh]]
		+	[[Category: Hhq]]
		+	[[Category: Pseudomonas quinolone signal]]
		+	[[Category: Transferase]]

OCA: Protected "6esz" [edit=sysop:move=sysop]

OCA — Wed, 01 Nov 2017 05:06:13 GMT

Protected "6esz" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:06, 1 November 2017

OCA: New page: '''Unreleased structure''' The entry 6esz is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Nov 2017 05:06:12 GMT

New page: '''Unreleased structure''' The entry 6esz is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6esz is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]