6ewn - Revision history

OCA at 07:51, 24 April 2019

OCA — Wed, 24 Apr 2019 07:51:37 GMT

←Older revision		Revision as of 07:51, 24 April 2019
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	==HspA from Thermosynechococcus vulcanus in the presence of 2M urea with initial stages of denaturation==		==HspA from Thermosynechococcus vulcanus in the presence of 2M urea with initial stages of denaturation==
-	<StructureSection load='6ewn' size='340' side='right' caption='[[6ewn]], [[Resolution\|resolution]] 2.29Å' scene=''>	+	<StructureSection load='6ewn' size='340' side='right'caption='[[6ewn]], [[Resolution\|resolution]] 2.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ewn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EWN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ewn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechococcus_vulcanus Synechococcus vulcanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EWN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=URE:UREA'>URE</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=URE:UREA'>URE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hspA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32053 Synechococcus vulcanus])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ewn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ewn OCA], [http://pdbe.org/6ewn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ewn RCSB], [http://www.ebi.ac.uk/pdbsum/6ewn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ewn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ewn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ewn OCA], [http://pdbe.org/6ewn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ewn RCSB], [http://www.ebi.ac.uk/pdbsum/6ewn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ewn ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Small heat shock proteins (sHSPs) belong to the superfamily of molecular chaperones. They prevent aggregation of partially unfolded or misfolded client proteins, providing protection to organisms under stress conditions. Here, we report the biophysical and structural characterization of a small heat shock protein (HspA) from a thermophilic cyanobacterium Thermosynechococcus vulcanus in the presence of 2M urea. HspA has been shown to be important for the protection of Photosystem II and the Phycobilisome antenna complex at elevated temperatures. Heterologously expressed HspA requires the presence of 1-2M urea to maintain its solubility at concentrations required for most characterization methods. Spectroscopic studies reveal the presence of the beta-sheet structure and intactness of the tertiary fold in HspA. In vitro assays show that the HspA maintains chaperone-like activity in protecting soluble proteins from thermal aggregation. Chromatography and electron microscopy show that the HspA exists as a mixture of oligomeric forms in the presence of 2M urea. HspA was successfully crystallized only in the presence of 2M urea. The crystal structure of HspA shows urea-induced loss of about 30% of the secondary structure without major alteration in the tertiary structure of the protein. The electron density maps reveal changes in the hydrogen bonding network which we attribute to the presence of urea. The crystal structure of HspA demonstrates a mixture of both direct interactions between urea and protein functionalities and interactions between urea and the surrounding solvent that indirectly affect the protein, which are in accordance with previously published studies.
		+
		+	Biophysical and structural characterization of the small heat shock protein HspA from Thermosynechococcus vulcanus in 2 M urea.,Ghosh S, Salama F, Dines M, Lahav A, Adir N Biochim Biophys Acta Proteins Proteom. 2019 Apr;1867(4):442-452. doi:, 10.1016/j.bbapap.2018.12.011. Epub 2019 Jan 31. PMID:30711645<ref>PMID:30711645</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6ewn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Synechococcus vulcanus]]
	[[Category: Adir, N]]		[[Category: Adir, N]]
	[[Category: Dines, M]]		[[Category: Dines, M]]

OCA at 08:04, 17 October 2018

OCA — Wed, 17 Oct 2018 08:04:25 GMT

←Older revision		Revision as of 08:04, 17 October 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6ewn is ~~ON HOLD until Paper Publication~~	+	==HspA from Thermosynechococcus vulcanus in the presence of 2M urea with initial stages of denaturation==
-		+	<StructureSection load='6ewn' size='340' side='right' caption='[[6ewn]], [[Resolution\|resolution]] 2.29Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6ewn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EWN FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=URE:UREA'>URE</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ewn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ewn OCA], [http://pdbe.org/6ewn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ewn RCSB], [http://www.ebi.ac.uk/pdbsum/6ewn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ewn ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Adir, N]]
		+	[[Category: Dines, M]]
		+	[[Category: Ghosh, S]]
		+	[[Category: Salama, F]]
		+	[[Category: Chaperone]]
		+	[[Category: Cyanobacteria]]
		+	[[Category: Denaturation]]
		+	[[Category: Small heat shock protein]]
		+	[[Category: Urea]]

OCA at 06:09, 6 December 2017

OCA — Wed, 06 Dec 2017 06:09:48 GMT

←Older revision		Revision as of 06:09, 6 December 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ewn is ON HOLD	+	The entry 6ewn is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6ewn" [edit=sysop:move=sysop]

OCA — Wed, 15 Nov 2017 06:29:57 GMT

Protected "6ewn" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:29, 15 November 2017

OCA: New page: '''Unreleased structure''' The entry 6ewn is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Nov 2017 06:29:56 GMT

New page: '''Unreleased structure''' The entry 6ewn is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ewn is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]