6ey6 - Revision history

OCA at 06:23, 8 March 2018

OCA — Thu, 08 Mar 2018 06:23:52 GMT

←Older revision		Revision as of 06:23, 8 March 2018
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-	==C-terminal part (residues 315-516) of PorM with the llama ~~naobody~~ nb130==	+	==C-terminal part (residues 315-516) of PorM with the llama nanobody nb130==
	<StructureSection load='6ey6' size='340' side='right' caption='[[6ey6]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6ey6' size='340' side='right' caption='[[6ey6]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==

OCA at 07:10, 15 February 2018

OCA — Thu, 15 Feb 2018 07:10:41 GMT

←Older revision		Revision as of 07:10, 15 February 2018
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	<StructureSection load='6ey6' size='340' side='right' caption='[[6ey6]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6ey6' size='340' side='right' caption='[[6ey6]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ey6]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EY6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ey6]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33277 Atcc 33277] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EY6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ey6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ey6 OCA], [http://pdbe.org/6ey6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ey6 RCSB], [http://www.ebi.ac.uk/pdbsum/6ey6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ey6 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">porM, PGIN_15-9_01458 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=837 ATCC 33277])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ey6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ey6 OCA], [http://pdbe.org/6ey6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ey6 RCSB], [http://www.ebi.ac.uk/pdbsum/6ey6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ey6 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	~~PorM is a membrane protein that is involved in the assembly of the type~~ IX secretion system (T9SS) in ~~Porphyromonas gingivalis~~, ~~a major bacterial pathogen that is responsible for periodontal disease~~ in ~~humans~~. In the ~~context of structural studies of PorM to better understand~~ T9SS ~~assembly~~, four ~~camelid nanobodies were selected~~, ~~produced and purified, and their specific interaction with the N~~-~~terminal~~ or C-~~terminal part of~~ the ~~periplasmic domain~~ of PorM ~~was investigated. Diffracting crystals were also obtained~~, and the structures of the ~~four nanobodies were solved by molecular replacement~~. ~~Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in~~ the structure-~~determination process~~ of the periplasmic ~~domain~~ of ~~PorM~~.	+	Type IX secretion system (T9SS), exclusively present in the Bacteroidetes phylum, has been studied mainly in Flavobacterium johnsoniae and Porphyromonas gingivalis. Among the 18 genes, essential for T9SS function, a group of four, porK-N (P. gingivalis) or gldK-N (F. johnsoniae) belongs to a co-transcribed operon that expresses the T9SS core membrane complex. The central component of this complex, PorM (or GldM), is anchored in the inner membrane by a trans-membrane helix and interacts through the outer membrane PorK-N complex. There is a complete lack of available atomic structures for any component of T9SS, including the PorKLMN complex. Here we report the crystal structure of the GldM and PorM periplasmic domains. Dimeric GldM and PorM, each contain four domains of ~180-A length that span most of the periplasmic space. These and previously reported results allow us to propose a model of the T9SS core membrane complex as well as its functional behavior.

-	~~Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type~~ IX secretion system ~~from Porphyromonas gingivalis~~.,~~Duhoo Y~~, Roche J, ~~Trinh TTN~~, Desmyter A, ~~Gaubert A~~, Kellenberger C, Cambillau C, Roussel A~~, Leone P Acta Crystallogr F Struct Biol~~ Commun. ~~2017 May 1~~;73(~~Pt 5~~):~~286-293~~. doi:, 10.~~1107~~/~~S2053230X17005969. Epub 2017 Apr 26~~. PMID:~~28471361~~<ref>PMID:~~28471361~~</ref>	+	Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space.,Leone P, Roche J, Vincent MS, Tran QH, Desmyter A, Cascales E, Kellenberger C, Cambillau C, Roussel A Nat Commun. 2018 Jan 30;9(1):429. doi: 10.1038/s41467-017-02784-7. PMID:29382829<ref>PMID:29382829</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 33277]]
		+	[[Category: Camelus glama]]
	[[Category: Cambillau, C]]		[[Category: Cambillau, C]]
	[[Category: Leone, P]]		[[Category: Leone, P]]

OCA at 06:24, 7 February 2018

OCA — Wed, 07 Feb 2018 06:24:20 GMT

←Older revision		Revision as of 06:24, 7 February 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6ey6 is ~~ON HOLD until Paper~~ Publication	+	==C-terminal part (residues 315-516) of PorM with the llama naobody nb130==
		+	<StructureSection load='6ey6' size='340' side='right' caption='[[6ey6]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ey6]] is a 16 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6EY6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6EY6 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ey6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ey6 OCA], [http://pdbe.org/6ey6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ey6 RCSB], [http://www.ebi.ac.uk/pdbsum/6ey6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ey6 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM.

-	~~Authors: Leone~~, P., ~~Cambillau~~, C., ~~Roussel~~, A.	+	Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis.,Duhoo Y, Roche J, Trinh TTN, Desmyter A, Gaubert A, Kellenberger C, Cambillau C, Roussel A, Leone P Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):286-293. doi:, 10.1107/S2053230X17005969. Epub 2017 Apr 26. PMID:28471361<ref>PMID:28471361</ref>

-	~~Description: C-terminal part (residues 315-516)~~ of ~~PorM with~~ the ~~llama naobody nb130~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Roussel, A]]~~	+	<div class="pdbe-citations 6ey6" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Cambillau, C]]		[[Category: Cambillau, C]]
	[[Category: Leone, P]]		[[Category: Leone, P]]
		+	[[Category: Roussel, A]]
		+	[[Category: Nanobody]]
		+	[[Category: Protein transport]]

OCA: Protected "6ey6" [edit=sysop:move=sysop]

OCA — Wed, 22 Nov 2017 06:41:42 GMT

Protected "6ey6" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:41, 22 November 2017

OCA: New page: '''Unreleased structure''' The entry 6ey6 is ON HOLD until Paper Publication Authors: Leone, P., Cambillau, C., Roussel, A. Description: C-terminal part (residues 315-516) of PorM with...

OCA — Wed, 22 Nov 2017 06:41:41 GMT

New page: '''Unreleased structure''' The entry 6ey6 is ON HOLD until Paper Publication Authors: Leone, P., Cambillau, C., Roussel, A. Description: C-terminal part (residues 315-516) of PorM with...

New page

'''Unreleased structure'''

The entry 6ey6 is ON HOLD until Paper Publication

Authors: Leone, P., Cambillau, C., Roussel, A.

Description: C-terminal part (residues 315-516) of PorM with the llama naobody nb130
[[Category: Unreleased Structures]]
[[Category: Roussel, A]]
[[Category: Cambillau, C]]
[[Category: Leone, P]]