6f0f - Revision history

OCA at 11:16, 1 January 2020

OCA — Wed, 01 Jan 2020 11:16:24 GMT

←Older revision		Revision as of 11:16, 1 January 2020
Line 3:		Line 3:
	<StructureSection load='6f0f' size='340' side='right'caption='[[6f0f]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6f0f' size='340' side='right'caption='[[6f0f]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6f0f]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F0F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F0F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6f0f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F0F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F0F FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f0e\|6f0e]], [[6f0h\|6f0h]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f0e\|6f0e]], [[6f0h\|6f0h]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ASF1A, CGI-98, HSPC146 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f0f OCA], [http://pdbe.org/6f0f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f0f RCSB], [http://www.ebi.ac.uk/pdbsum/6f0f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f0f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f0f OCA], [http://pdbe.org/6f0f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f0f RCSB], [http://www.ebi.ac.uk/pdbsum/6f0f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f0f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ASF1A_HUMAN ASF1A_HUMAN]] Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.<ref>PMID:10759893</ref> <ref>PMID:11897662</ref> <ref>PMID:12842904</ref> <ref>PMID:14718166</ref> <ref>PMID:15621527</ref> <ref>PMID:16151251</ref> <ref>PMID:15664198</ref>		[[http://www.uniprot.org/uniprot/ASF1A_HUMAN ASF1A_HUMAN]] Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.<ref>PMID:10759893</ref> <ref>PMID:11897662</ref> <ref>PMID:12842904</ref> <ref>PMID:14718166</ref> <ref>PMID:15621527</ref> <ref>PMID:16151251</ref> <ref>PMID:15664198</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Anti-silencing function 1 (ASF1) is a conserved H3-H4 histone chaperone involved in histone dynamics during replication, transcription, and DNA repair. Overexpressed in proliferating tissues including many tumors, ASF1 has emerged as a promising therapeutic target. Here, we combine structural, computational, and biochemical approaches to design peptides that inhibit the ASF1-histone interaction. Starting from the structure of the human ASF1-histone complex, we developed a rational design strategy combining epitope tethering and optimization of interface contacts to identify a potent peptide inhibitor with a dissociation constant of 3 nM. When introduced into cultured cells, the inhibitors impair cell proliferation, perturb cell-cycle progression, and reduce cell migration and invasion in a manner commensurate with their affinity for ASF1. Finally, we find that direct injection of the most potent ASF1 peptide inhibitor in mouse allografts reduces tumor growth. Our results open new avenues to use ASF1 inhibitors as promising leads for cancer therapy.
		+
		+	Design on a Rational Basis of High-Affinity Peptides Inhibiting the Histone Chaperone ASF1.,Bakail M, Gaubert A, Andreani J, Moal G, Pinna G, Boyarchuk E, Gaillard MC, Courbeyrette R, Mann C, Thuret JY, Guichard B, Murciano B, Richet N, Poitou A, Frederic C, Le Du MH, Agez M, Roelants C, Gurard-Levin ZA, Almouzni G, Cherradi N, Guerois R, Ochsenbein F Cell Chem Biol. 2019 Nov 21;26(11):1573-1585.e10. doi:, 10.1016/j.chembiol.2019.09.002. Epub 2019 Sep 19. PMID:31543461<ref>PMID:31543461</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6f0f" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Andreani, J]]		[[Category: Andreani, J]]

OCA at 05:30, 12 June 2019

OCA — Wed, 12 Jun 2019 05:30:47 GMT

←Older revision		Revision as of 05:30, 12 June 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6f0f is ~~ON HOLD~~	+	==Crystal structure ASF1-ip2_s==
-		+	<StructureSection load='6f0f' size='340' side='right'caption='[[6f0f]], [[Resolution\|resolution]] 2.00Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6f0f]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F0F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F0F FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6f0e\|6f0e]], [[6f0h\|6f0h]]</td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f0f OCA], [http://pdbe.org/6f0f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f0f RCSB], [http://www.ebi.ac.uk/pdbsum/6f0f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f0f ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ASF1A_HUMAN ASF1A_HUMAN]] Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.<ref>PMID:10759893</ref> <ref>PMID:11897662</ref> <ref>PMID:12842904</ref> <ref>PMID:14718166</ref> <ref>PMID:15621527</ref> <ref>PMID:16151251</ref> <ref>PMID:15664198</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Andreani, J]]
		+	[[Category: Du, M H.Le]]
		+	[[Category: Gaubert, A]]
		+	[[Category: Guerois, R]]
		+	[[Category: Guichard, B]]
		+	[[Category: Murciano, B]]
		+	[[Category: Ochsenbein, F]]
		+	[[Category: Chaperone]]
		+	[[Category: Protein-peptide complexe]]

OCA: Protected "6f0f" [edit=sysop:move=sysop]

OCA — Wed, 29 Nov 2017 05:13:17 GMT

Protected "6f0f" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:13, 29 November 2017

OCA: New page: '''Unreleased structure''' The entry 6f0f is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Nov 2017 05:13:16 GMT

New page: '''Unreleased structure''' The entry 6f0f is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6f0f is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]