OCA at 07:43, 21 March 2018

OCA — Wed, 21 Mar 2018 07:43:24 GMT

←Older revision		Revision as of 07:43, 21 March 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6f0q is ~~ON HOLD~~	+	==Crystal structure of Pizza6-AYW==
		+	<StructureSection load='6f0q' size='340' side='right' caption='[[6f0q]], [[Resolution\|resolution]] 1.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6f0q]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F0Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F0Q FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f0q OCA], [http://pdbe.org/6f0q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f0q RCSB], [http://www.ebi.ac.uk/pdbsum/6f0q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f0q ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	beta-propeller proteins are highly symmetrical, being composed of a repeated motif with four anti-parallel beta-sheets arranged around a central axis. Recently we designed the first completely symmetrical beta-propeller protein, Pizza6, consisting of six identical tandem repeats. Pizza6 is expected to prove a useful building block for bionanotechnology, and also a tool to investigate the folding and evolution of beta-propeller proteins. Folding studies are made difficult by the high stability and the lack of buried Trp residues to act as monitor fluorophores, so we have designed and characterized several Trp-containing Pizza6 derivatives. In total four proteins were designed, of which three could be purified and characterized. Crystal structures confirm these mutant proteins maintain the expected structure, and a clear redshift of Trp fluorescence emission could be observed upon denaturation. Among the derivative proteins, Pizza6-AYW appears to be the most suitable model protein for future folding/unfolding kinetics studies as it has a comparable stability as natural beta-propeller proteins.

-	~~Authors: Noguchi~~, H., De Zitter, E., Van Meervelt, L., ~~Voet~~, A.R.D.	+	Design of tryptophan-containing mutants of the symmetrical Pizza protein for biophysical studies.,Noguchi H, Mylemans B, De Zitter E, Van Meervelt L, Tame JRH, Voet A Biochem Biophys Res Commun. 2018 Mar 18;497(4):1038-1042. doi:, 10.1016/j.bbrc.2018.02.168. Epub 2018 Feb 23. PMID:29481797<ref>PMID:29481797</ref>

-	~~Description: Crystal structure~~ of ~~Pizza6~~-~~AYW~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6f0q" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Meervelt, L Van]]
	[[Category: Noguchi, H]]		[[Category: Noguchi, H]]
-	[[Category: Voet, A.R.D]]	+	[[Category: Voet, A R.D]]
-	[[Category: ~~Van Meervelt~~, L]]	+	[[Category: Zitter, E De]]
-	[[Category: De ~~Zitter, E~~]]	+	[[Category: Artificial protein]]
		+	[[Category: Beta propeller]]
		+	[[Category: De novo protein]]

OCA: Protected "6f0q" [edit=sysop:move=sysop]

OCA — Wed, 29 Nov 2017 05:14:15 GMT

Protected "6f0q" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:14, 29 November 2017

OCA: New page: '''Unreleased structure''' The entry 6f0q is ON HOLD Authors: Noguchi, H., De Zitter, E., Van Meervelt, L., Voet, A.R.D. Description: Crystal structure of Pizza6-AYW [[Category: Unrele...

OCA — Wed, 29 Nov 2017 05:14:13 GMT

New page: '''Unreleased structure''' The entry 6f0q is ON HOLD Authors: Noguchi, H., De Zitter, E., Van Meervelt, L., Voet, A.R.D. Description: Crystal structure of Pizza6-AYW [[Category: Unrele...

New page

'''Unreleased structure'''

The entry 6f0q is ON HOLD

Authors: Noguchi, H., De Zitter, E., Van Meervelt, L., Voet, A.R.D.

Description: Crystal structure of Pizza6-AYW
[[Category: Unreleased Structures]]
[[Category: Noguchi, H]]
[[Category: Voet, A.R.D]]
[[Category: Van Meervelt, L]]
[[Category: De Zitter, E]]

6f0q - Revision history

OCA at 07:43, 21 March 2018

OCA: Protected "6f0q" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6f0q is ON HOLD Authors: Noguchi, H., De Zitter, E., Van Meervelt, L., Voet, A.R.D. Description: Crystal structure of Pizza6-AYW [[Category: Unrele...