OCA at 06:20, 9 May 2018

OCA — Wed, 09 May 2018 06:20:38 GMT

←Older revision		Revision as of 06:20, 9 May 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6f1l is ~~ON HOLD~~	+	==Lysozyme crystallized in presence of 100 mM sodium phosphate at pH 4.5==
		+	<StructureSection load='6f1l' size='340' side='right' caption='[[6f1l]], [[Resolution\|resolution]] 1.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6f1l]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F1L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F1L FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f1l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f1l OCA], [http://pdbe.org/6f1l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f1l RCSB], [http://www.ebi.ac.uk/pdbsum/6f1l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f1l ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structure of orthorhombic lysozyme has been obtained at 298 K and pH 4.5 using sodium chloride as the precipitant and in the presence of sodium phosphate at a concentration as low as 5 mM. Crystals belonging to space group P212121 (unit-cell parameters a = 30, b = 56, c = 73 A, alpha = beta = gamma = 90.00 degrees ) diffracted to a resolution higher than 1 A, and the high quality of these crystals permitted the identification of a phosphate ion bound to Arg14 and His15. The binding of this ion produces long-range conformational changes affecting the loop containing Ser60-Asn74. The negatively charged phosphate ion shields the electrostatic repulsion of the positively charged arginine and histidine residues, resulting in higher stability of the phosphate-bound lysozyme. Additionally, a low-humidity orthorhombic variant was obtained at pH 4.5, and comparison with those previously obtained at pH 6.5 and 9.5 shows a 1.5 A displacement of the fifth alpha-helix towards the active-site cavity, which might be relevant to protein function. Since lysozyme is broadly used as a model protein in studies related to protein crystallization and amyloid formation, these results indicate that the interaction of some anions must be considered when analysing experiments performed at acidic pH values.

-	~~Authors:~~ Camara-Artigas, A.	+	Orthorhombic lysozyme crystallization at acidic pH values driven by phosphate binding.,Plaza-Garrido M, Salinas-Garcia MC, Camara-Artigas A Acta Crystallogr D Struct Biol. 2018 May 1;74(Pt 5):480-489. doi:, 10.1107/S205979831800517X. Epub 2018 Apr 27. PMID:29717719<ref>PMID:29717719</ref>

-	~~Description: Lysozyme crystallized in presence~~ of ~~100 mM sodium phosphate at pH 4~~.5	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6f1l" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Lysozyme]]
	[[Category: Camara-Artigas, A]]		[[Category: Camara-Artigas, A]]
		+	[[Category: Hydrolase]]

OCA: Protected "6f1l" [edit=sysop:move=sysop]

OCA — Wed, 29 Nov 2017 05:16:15 GMT

Protected "6f1l" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:16, 29 November 2017

OCA: New page: '''Unreleased structure''' The entry 6f1l is ON HOLD Authors: Camara-Artigas, A. Description: Lysozyme crystallized in presence of 100 mM sodium phosphate at pH 4.5 [[Category: Unrelea...

OCA — Wed, 29 Nov 2017 05:16:14 GMT

New page: '''Unreleased structure''' The entry 6f1l is ON HOLD Authors: Camara-Artigas, A. Description: Lysozyme crystallized in presence of 100 mM sodium phosphate at pH 4.5 [[Category: Unrelea...

New page

'''Unreleased structure'''

The entry 6f1l is ON HOLD

Authors: Camara-Artigas, A.

Description: Lysozyme crystallized in presence of 100 mM sodium phosphate at pH 4.5
[[Category: Unreleased Structures]]
[[Category: Camara-Artigas, A]]

6f1l - Revision history

OCA at 06:20, 9 May 2018

OCA: Protected "6f1l" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6f1l is ON HOLD Authors: Camara-Artigas, A. Description: Lysozyme crystallized in presence of 100 mM sodium phosphate at pH 4.5 [[Category: Unrelea...