6f49 - Revision history

OCA at 22:13, 9 August 2018

OCA — Thu, 09 Aug 2018 22:13:01 GMT

←Older revision		Revision as of 22:13, 9 August 2018
Line 3:		Line 3:
	<StructureSection load='6f49' size='340' side='right' caption='[[6f49]], [[Resolution\|resolution]] 2.02Å' scene=''>		<StructureSection load='6f49' size='340' side='right' caption='[[6f49]], [[Resolution\|resolution]] 2.02Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6f49]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F49 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F49 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6f49]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F49 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F49 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lolC, ycfU, b1116, JW5161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f49 OCA], [http://pdbe.org/6f49 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f49 RCSB], [http://www.ebi.ac.uk/pdbsum/6f49 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f49 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f49 OCA], [http://pdbe.org/6f49 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f49 RCSB], [http://www.ebi.ac.uk/pdbsum/6f49 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f49 ProSAT]</span></td></tr>
	</table>		</table>
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Kaplan, E]]		[[Category: Kaplan, E]]
	[[Category: Lipoprotein trafficking]]		[[Category: Lipoprotein trafficking]]
	[[Category: Protein transport]]		[[Category: Protein transport]]

OCA at 07:21, 25 July 2018

OCA — Wed, 25 Jul 2018 07:21:44 GMT

←Older revision		Revision as of 07:21, 25 July 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6f49 is ~~ON HOLD until Paper~~ Publication	+	==Periplasmic domain of LolC lacking the Hook.==
		+	<StructureSection load='6f49' size='340' side='right' caption='[[6f49]], [[Resolution\|resolution]] 2.02Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6f49]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F49 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F49 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG5:1-METHOXY-2-[2-(2-METHOXY-ETHOXY]-ETHANE'>PG5</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f49 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f49 OCA], [http://pdbe.org/6f49 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f49 RCSB], [http://www.ebi.ac.uk/pdbsum/6f49 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f49 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LOLC_ECOLI LOLC_ECOLI]] Part of an ATP-dependent transport system LolCDE responsible for the release of lipoproteins targeted to the outer membrane from the inner membrane. Such a release is dependent of the sorting-signal (absence of an Asp at position 2 of the mature lipoprotein) and of LolA.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In Gram-negative bacteria, outer-membrane lipoproteins are essential for maintaining cellular integrity, transporting nutrients, establishing infections, and promoting the formation of biofilms. The LolCDE ABC transporter, LolA chaperone, and LolB outer-membrane receptor form an essential system for transporting newly matured lipoproteins from the outer leaflet of the cytoplasmic membrane to the innermost leaflet of the outer membrane. Here, we present a crystal structure of LolA in complex with the periplasmic domain of LolC. The structure reveals how a solvent-exposed beta-hairpin loop (termed the "Hook") and trio of surface residues (the "Pad") of LolC are essential for recruiting LolA from the periplasm and priming it to receive lipoproteins. Experiments with purified LolCDE complex demonstrate that association with LolA is independent of nucleotide binding and hydrolysis, and homology models based on the MacB ABC transporter predict that LolA recruitment takes place at a periplasmic site located at least 50 A from the inner membrane. Implications for the mechanism of lipoprotein extraction and transfer are discussed. The LolA-LolC structure provides atomic details on a key protein interaction within the Lol pathway and constitutes a vital step toward the complete molecular understanding of this important system.

-	~~Authors~~:	+	Insights into bacterial lipoprotein trafficking from a structure of LolA bound to the LolC periplasmic domain.,Kaplan E, Greene NP, Crow A, Koronakis V Proc Natl Acad Sci U S A. 2018 Jul 16. pii: 1806822115. doi:, 10.1073/pnas.1806822115. PMID:30012603<ref>PMID:30012603</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6f49" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Kaplan, E]]
		+	[[Category: Lipoprotein trafficking]]
		+	[[Category: Protein transport]]

OCA at 05:00, 9 May 2018

OCA — Wed, 09 May 2018 05:00:01 GMT

←Older revision		Revision as of 05:00, 9 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6f49 is ON HOLD	+	The entry 6f49 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6f49" [edit=sysop:move=sysop]

OCA — Wed, 06 Dec 2017 06:16:43 GMT

Protected "6f49" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:16, 6 December 2017

OCA: New page: '''Unreleased structure''' The entry 6f49 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 Dec 2017 06:16:41 GMT

New page: '''Unreleased structure''' The entry 6f49 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6f49 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]